FastQCFastQC Report
Wed 25 May 2016
SRR2088559_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088559_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295577
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT722311.6815203172938116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT507741.182006515073528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT503871.1729972480996151No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212710.4951837669304962No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG110430.25707838551142254No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA108660.25295786805823756No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA90670.2110775804973348No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG88870.2068872237652823No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG83300.19392039765554198No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA78990.1838868212582384No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA72820.16952320957114725No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT63530.14789631288183172No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC61310.1427282062456336No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA53270.12401127950913231No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT47410.11036934037033908No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45700.10638850147488917No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA45490.10589962652281638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA187500.019.7234671
GTATTGG10600.019.3726431
TATACCG3350.018.776125
ATTGGAC10750.018.5860483
TTGGACC15800.016.2753164
TATTGGA14200.015.373242
TATACAC20250.015.07407537
ACCGTTA3700.014.58
TGGACCC18850.014.4270565
GGACCCT17450.014.4183376
CGTTTAC3550.014.07042328
CGACGAG2457.2759576E-1213.59183624
GACCCTC17600.013.4545457
GTCTTAC3500.013.2142851
CGCGATA1551.8907504E-613.12903214
TACCGAC8950.013.02234657
CCGTTTA3850.012.97402627
ACGTTTA2001.4657417E-812.94999926
GACCGTT4000.012.9499997
TACGTTA2305.7661964E-1012.86956519
Produced by FastQC (version 0.11.5)