##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088559_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4295577
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82620751531168	31.0	31.0	34.0	30.0	34.0
2	31.980736231709965	31.0	31.0	34.0	30.0	34.0
3	32.10409614354486	33.0	31.0	34.0	30.0	34.0
4	35.75387543978376	37.0	35.0	37.0	35.0	37.0
5	35.67509859560194	37.0	35.0	37.0	33.0	37.0
6	35.72759934230023	37.0	35.0	37.0	33.0	37.0
7	35.59648284735671	37.0	35.0	37.0	33.0	37.0
8	35.63530394170562	37.0	35.0	37.0	33.0	37.0
9	37.26157021513059	39.0	37.0	39.0	34.0	39.0
10	37.12712448176345	39.0	37.0	39.0	33.0	39.0
11	37.18364541015095	39.0	37.0	39.0	33.0	39.0
12	37.044176137454876	39.0	37.0	39.0	33.0	39.0
13	37.101864312989846	39.0	37.0	39.0	33.0	39.0
14	38.15004014594547	40.0	37.0	41.0	33.0	41.0
15	38.156000928396814	40.0	37.0	41.0	33.0	41.0
16	38.052812229882036	40.0	37.0	41.0	33.0	41.0
17	38.08751257398016	40.0	37.0	41.0	33.0	41.0
18	38.05966555831731	40.0	37.0	41.0	33.0	41.0
19	38.119839779382374	40.0	37.0	41.0	33.0	41.0
20	38.03209789976992	40.0	37.0	41.0	33.0	41.0
21	37.903338713285784	40.0	37.0	41.0	32.0	41.0
22	37.78631322404417	40.0	37.0	41.0	32.0	41.0
23	37.624163878333455	40.0	37.0	41.0	32.0	41.0
24	37.78096283689013	40.0	37.0	41.0	32.0	41.0
25	37.72727272727273	40.0	37.0	41.0	32.0	41.0
26	37.545383309390104	40.0	37.0	41.0	32.0	41.0
27	37.49946258674911	40.0	36.0	41.0	32.0	41.0
28	37.362201166455634	39.0	36.0	41.0	32.0	41.0
29	37.152433305234666	39.0	36.0	41.0	31.0	41.0
30	37.013560925575305	39.0	36.0	41.0	31.0	41.0
31	36.76024082445734	39.0	35.0	41.0	30.0	41.0
32	36.607186880831144	39.0	35.0	40.0	30.0	41.0
33	36.43983031848806	39.0	35.0	40.0	30.0	41.0
34	36.25819534837811	38.0	35.0	40.0	30.0	41.0
35	36.04856390654852	38.0	35.0	40.0	30.0	41.0
36	35.96356764178596	38.0	35.0	40.0	29.0	41.0
37	35.82377152126478	38.0	35.0	40.0	28.0	41.0
38	35.67587637237093	38.0	35.0	40.0	27.0	41.0
39	35.536432707410434	38.0	35.0	40.0	26.0	41.0
40	35.3404397127557	38.0	35.0	40.0	25.0	41.0
41	35.183412612554726	38.0	34.0	40.0	24.0	41.0
42	34.89364874614051	38.0	34.0	40.0	23.0	41.0
43	34.49931708825147	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	7.0
12	11.0
13	11.0
14	10.0
15	35.0
16	54.0
17	127.0
18	244.0
19	591.0
20	1224.0
21	2438.0
22	4705.0
23	8233.0
24	13465.0
25	20656.0
26	30369.0
27	43074.0
28	59371.0
29	79314.0
30	100486.0
31	124918.0
32	154628.0
33	193973.0
34	244007.0
35	307740.0
36	412764.0
37	615878.0
38	824771.0
39	1052469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.96167360054307	20.857477354031833	14.35376434877084	22.827084696654257
2	18.2207186601474	23.996077826098798	35.62806579884379	22.155137714910012
3	19.213809925884227	24.369578289482412	33.643186933909	22.77342485072436
4	13.163353840473585	16.251786430554034	36.58814636543589	33.99671336353649
5	12.43011125164326	38.90934791763714	34.78517554219142	13.875365288528178
6	31.836677587201905	38.12558825042596	13.7538915028179	16.283842659554235
7	25.4402842738007	34.103264823328736	21.308266619362197	19.148184283508364
8	27.536207592134886	34.31764812969247	18.95510195719923	19.191042320973413
9	24.801464389999296	14.503569601941718	21.824309050914465	38.870656957144526
10	16.28016445753388	28.40612564970899	32.90142395305683	22.4122859397003
11	33.22282431440526	23.05252588883868	23.28045801530272	20.44419178145334
12	21.443196106134284	27.915411596626015	30.75477403850519	19.886618258734508
13	31.99106895301842	21.099679973144468	25.932558070778384	20.976693003058728
14	20.4773421591558	23.208546837828774	28.673982563925637	27.64012843908979
15	22.60301701028756	30.346842810639874	25.291899085966797	21.758241093105767
16	21.36120479274379	28.56964268129753	26.69683257918552	23.372319946773157
17	21.4449653678656	27.3953883261783	27.46708998581564	23.69255632014046
18	22.62916018034364	25.76212695058196	29.996575547359527	21.612137321714872
19	22.438149752640914	25.22671575902376	31.520678130085898	20.814456358249426
20	23.223026848313975	25.214074849548734	30.62387195014779	20.9390263519895
21	22.11197704056987	25.849146692050915	31.339491760943872	20.69938450643534
22	21.068997249961996	26.35967182057265	30.901622762204006	21.669708167261348
23	21.95344187754055	26.272954715978784	31.31111373396403	20.462489672516636
24	21.482189703501998	26.467433827865268	29.846374538275068	22.204001930357666
25	21.721505632421444	26.440033550789565	30.805267837126422	21.03319297966257
26	20.94216911953854	27.558137125699293	30.69950788916134	20.800185865600827
27	20.217959077441748	27.39068581473455	30.659489982370236	21.73186512545346
28	20.852006610520544	27.236550526273884	30.364558707712607	21.546884155492965
29	20.72606311096274	27.323779785579443	29.833221474088344	22.116935629369465
30	20.83873714753571	27.65672690770064	31.31197508507006	20.192560859693586
31	21.693546641114803	27.35911846068642	30.84388895834017	20.103445939858602
32	21.05004752562927	27.304224787496533	30.160697852698252	21.485029834175943
33	20.727157259665	27.11731159748737	30.801333557750215	21.354197585097413
34	20.091154226777917	27.49625952462265	31.28317802241701	21.12940822618242
35	19.61755079701749	29.364855990242987	30.458399418750965	20.559193793988563
36	21.579010223772034	27.494560102170208	30.125103100235428	20.80132657382233
37	20.764660952416868	28.032206150652172	30.198946497758044	21.004186399172916
38	20.41215883221276	26.84328554697076	31.370244323405217	21.374311297411268
39	20.07897891249534	26.627435615750805	32.059627845106725	21.23395762664713
40	18.982828150909643	26.84668439187564	32.098505043676326	22.07198241353839
41	19.97051851241405	25.987032708295065	32.288444602436414	21.754004176854473
42	19.098295758637317	27.56188516699852	31.599014521215658	21.740804553148507
43	19.01844618313209	27.417760175175538	31.167896652766323	22.39589698892605
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1228.0
1	1190.0
2	1152.0
3	2788.5
4	4425.0
5	4425.0
6	6712.5
7	9000.0
8	9635.0
9	10270.0
10	15629.5
11	20989.0
12	20989.0
13	38375.5
14	55762.0
15	95532.0
16	135302.0
17	123219.5
18	111137.0
19	111137.0
20	112673.0
21	114209.0
22	92158.0
23	70107.0
24	78393.5
25	86680.0
26	86680.0
27	95245.5
28	103811.0
29	111117.5
30	118424.0
31	128006.5
32	137589.0
33	137589.0
34	150447.0
35	163305.0
36	177434.5
37	191564.0
38	201218.5
39	210873.0
40	210873.0
41	218087.5
42	225302.0
43	235255.5
44	245209.0
45	260233.0
46	275257.0
47	275257.0
48	346040.0
49	416823.0
50	390438.5
51	364054.0
52	331038.0
53	298022.0
54	298022.0
55	267776.0
56	237530.0
57	209351.0
58	181172.0
59	162715.0
60	144258.0
61	144258.0
62	127261.0
63	110264.0
64	96103.5
65	81943.0
66	70255.5
67	58568.0
68	58568.0
69	49949.0
70	41330.0
71	34791.0
72	28252.0
73	23215.5
74	18179.0
75	18179.0
76	14391.0
77	10603.0
78	8295.5
79	5988.0
80	4739.5
81	3491.0
82	3491.0
83	2615.5
84	1740.0
85	1297.5
86	855.0
87	668.0
88	481.0
89	481.0
90	361.0
91	241.0
92	176.5
93	112.0
94	79.5
95	47.0
96	47.0
97	32.5
98	18.0
99	14.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4295577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.03196842305416
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.7814498421493	32.927744380797265
2	13.189746961275898	11.615410434139498
3	4.917646037809133	6.496009051577076
4	2.277900240654428	4.012017258694532
5	1.2641380717208928	2.783124382819747
6	0.7845424924296132	2.072697015192296
7	0.5032151454585363	1.5510287376383016
8	0.3400446752145149	1.1978269121178584
9	0.26028486140666485	1.0314769318611565
>10	1.4392245452723815	11.849193635466678
>50	0.1373374244008312	4.200660886452836
>100	0.09253306616364798	7.700002605046033
>500	0.006578412804054871	1.9493682016071257
>1k	0.004615499226692738	4.169709039406463
>5k	4.2441372199473424E-4	1.3846704370860363
>10k+	3.1831029149605064E-4	5.059060090097123
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	72231	1.6815203172938116	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	50774	1.182006515073528	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	50387	1.1729972480996151	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21271	0.4951837669304962	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	11043	0.25707838551142254	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10866	0.25295786805823756	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9067	0.2110775804973348	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	8887	0.2068872237652823	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8330	0.19392039765554198	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7899	0.1838868212582384	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7282	0.16952320957114725	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6353	0.14789631288183172	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6131	0.1427282062456336	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5327	0.12401127950913231	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4741	0.11036934037033908	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4570	0.10638850147488917	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4549	0.10589962652281638	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.6559519245028084E-5	0.0
5	0.0	0.0	0.0	4.6559519245028084E-5	0.0
6	0.0	0.0	0.0	4.6559519245028084E-5	0.0
7	0.0	0.0	0.0	4.6559519245028084E-5	0.0
8	0.0	0.0	0.0	4.6559519245028084E-5	0.0
9	0.0	0.0	0.0	1.3967855773508426E-4	0.0
10	0.0	0.0	0.0	1.8623807698011234E-4	0.0
11	2.3279759622514042E-5	0.0	0.0	2.793571154701685E-4	0.0
12	2.3279759622514042E-5	0.0	0.0	3.491963943377106E-4	0.0
13	2.3279759622514042E-5	0.0	0.0	3.957559135827387E-4	0.0
14	2.3279759622514042E-5	0.0	0.0	6.052737501853651E-4	0.0
15	2.3279759622514042E-5	0.0	0.0	7.216725482979353E-4	0.0
16	2.3279759622514042E-5	0.0	0.0	9.079106252780476E-4	0.0
17	2.3279759622514042E-5	0.0	0.0	0.0011872677407482161	0.0
18	2.3279759622514042E-5	0.0	0.0	0.0014200653369733566	0.0
19	2.3279759622514042E-5	0.0	0.0	0.0017925414909335813	0.0
20	2.3279759622514042E-5	0.0	0.0	0.0032358865875294516	0.0
21	4.6559519245028084E-5	0.0	0.0	0.005866499424873538	0.0
22	6.983927886754213E-5	0.0	0.0	0.009242064570138075	0.0
23	6.983927886754213E-5	0.0	0.0	0.011220844138051769	0.0
24	6.983927886754213E-5	0.0	0.0	0.01485248663916396	0.0
25	6.983927886754213E-5	0.0	0.0	0.017529658995753074	0.0
26	6.983927886754213E-5	0.0	0.0	0.022907283468553818	0.0
27	6.983927886754213E-5	0.0	0.0	0.061970720115132376	0.0
28	6.983927886754213E-5	0.0	0.0	0.16435510293494912	0.0
29	6.983927886754213E-5	0.0	0.0	0.3388834608249369	0.0
30	9.311903849005617E-5	0.0	0.0	0.5632072245474822	0.0
31	9.311903849005617E-5	0.0	0.0	0.876901985460859	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	18750	0.0	19.723467	1
GTATTGG	1060	0.0	19.372643	1
TATACCG	335	0.0	18.77612	5
ATTGGAC	1075	0.0	18.586048	3
TTGGACC	1580	0.0	16.275316	4
TATTGGA	1420	0.0	15.37324	2
TATACAC	2025	0.0	15.074075	37
ACCGTTA	370	0.0	14.5	8
TGGACCC	1885	0.0	14.427056	5
GGACCCT	1745	0.0	14.418337	6
CGTTTAC	355	0.0	14.070423	28
CGACGAG	245	7.2759576E-12	13.591836	24
GACCCTC	1760	0.0	13.454545	7
GTCTTAC	350	0.0	13.214285	1
CGCGATA	155	1.8907504E-6	13.129032	14
TACCGAC	895	0.0	13.0223465	7
CCGTTTA	385	0.0	12.974026	27
ACGTTTA	200	1.4657417E-8	12.949999	26
GACCGTT	400	0.0	12.949999	7
TACGTTA	230	5.7661964E-10	12.869565	19
>>END_MODULE