FastQCFastQC Report
Wed 25 May 2016
SRR2088558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4808429
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT877141.824171678525356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT641661.334448319815058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT641511.3341363676161173No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274260.570373400543088No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA181810.37810686192933285No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG178310.3708279772873843No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG167590.34853379346975905No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA139780.29069785578616214No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG137500.2859561823622643No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA129180.26865323372768946No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA98630.20511896921011No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA91010.18927179750392487No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC84790.17633617965451917No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT79280.16487713554676592No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA79260.16483554192024047No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG63050.13112390762138737No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62210.1293769753073197No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC60630.1260910788118115No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA60200.12519681584151496No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT54130.11257315019104994No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA53880.11205322985948217No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG52070.10828900665893162No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC51800.10772749270083845No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA50510.10504470378994886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG20250.021.5604921
GGTATCA228050.021.2703361
TATAGCG500.0070380618.55
ATTGGAC23800.018.1890753
TTGGACC30950.017.5735074
TGGACCC32200.016.489135
GGACCCT31550.016.477026
TATTGGA25850.016.2456472
ACCGTTA2850.015.5789488
CGGTATA2055.456968E-1215.34146328
CGTTTAT3000.014.81
GACCCTC35900.014.1713097
TTTTTAC34350.014.0567681
GACGTAT2650.013.96226428
ATCGTAC1203.304657E-513.87499925
TCTATAC3750.013.8133343
TCTAACG2551.8189894E-1213.7843132
GTATCAA351600.013.7750292
CTCTAAT7300.013.6849321
GATACTG19850.013.51385436
Produced by FastQC (version 0.11.5)