##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088558_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4808429
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.788324419472556	31.0	31.0	34.0	30.0	34.0
2	31.932280792749566	31.0	31.0	34.0	30.0	34.0
3	32.05549546431901	33.0	31.0	34.0	30.0	34.0
4	35.70052339339938	37.0	35.0	37.0	35.0	37.0
5	35.639140767181964	37.0	35.0	37.0	33.0	37.0
6	35.702719536879925	37.0	35.0	37.0	33.0	37.0
7	35.55410072603755	37.0	35.0	37.0	33.0	37.0
8	35.605063940842214	37.0	35.0	37.0	33.0	37.0
9	37.21464723717455	39.0	37.0	39.0	33.0	39.0
10	37.08820261253727	39.0	37.0	39.0	33.0	39.0
11	37.14648817732361	39.0	37.0	39.0	33.0	39.0
12	37.00343708932793	39.0	37.0	39.0	33.0	39.0
13	37.0598667464987	39.0	37.0	39.0	33.0	39.0
14	38.083264201259915	40.0	37.0	41.0	33.0	41.0
15	38.08387250804785	40.0	37.0	41.0	33.0	41.0
16	37.983681988441546	40.0	37.0	41.0	33.0	41.0
17	38.03319296177608	40.0	37.0	41.0	33.0	41.0
18	38.00828753008519	40.0	37.0	41.0	33.0	41.0
19	38.07264451653544	40.0	37.0	41.0	33.0	41.0
20	37.9828894218881	40.0	37.0	41.0	33.0	41.0
21	37.846085280660276	40.0	37.0	41.0	32.0	41.0
22	37.73142038699126	40.0	37.0	41.0	32.0	41.0
23	37.54915649165247	39.0	36.0	41.0	32.0	41.0
24	37.6977310052826	40.0	37.0	41.0	32.0	41.0
25	37.640321818207156	40.0	37.0	41.0	32.0	41.0
26	37.44364011613772	39.0	36.0	41.0	32.0	41.0
27	37.408919212491234	39.0	36.0	41.0	32.0	41.0
28	37.2618884463096	39.0	36.0	41.0	31.0	41.0
29	37.04852645219468	39.0	36.0	41.0	31.0	41.0
30	36.88217253493813	39.0	35.0	41.0	31.0	41.0
31	36.596042283248856	39.0	35.0	40.0	30.0	41.0
32	36.43613724981694	38.0	35.0	40.0	30.0	41.0
33	36.254382876403085	38.0	35.0	40.0	30.0	41.0
34	36.047906291223185	38.0	35.0	40.0	30.0	41.0
35	35.8184951883453	38.0	35.0	40.0	29.0	41.0
36	35.734693181494414	38.0	35.0	40.0	27.0	41.0
37	35.564945640249654	38.0	35.0	40.0	27.0	41.0
38	35.41731031070647	38.0	35.0	40.0	26.0	41.0
39	35.27673133990332	38.0	34.0	40.0	25.0	41.0
40	35.069813654314125	38.0	34.0	40.0	24.0	41.0
41	34.90359512431191	38.0	34.0	40.0	23.0	41.0
42	34.57968309400014	38.0	33.0	40.0	22.0	41.0
43	34.19115952424378	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	16.0
14	18.0
15	36.0
16	38.0
17	112.0
18	276.0
19	691.0
20	1435.0
21	3030.0
22	5651.0
23	9717.0
24	16027.0
25	25187.0
26	37398.0
27	52900.0
28	72546.0
29	95446.0
30	119755.0
31	146636.0
32	178960.0
33	222387.0
34	277886.0
35	348818.0
36	464850.0
37	701543.0
38	915186.0
39	1111876.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.470877702467895	20.577427679601797	14.397924977159901	22.553769640770405
2	18.206528577213057	23.876488557905297	35.85118965050748	22.065793214374175
3	19.411745499413634	24.46994642116999	33.63649541253494	22.481812666881428
4	12.889698485721635	15.808531227143002	36.54807006612762	34.75370022100773
5	12.178634643456315	39.01319952940971	34.898695603075346	13.909470224058627
6	31.85608022911433	37.59379622741648	13.281323276271731	17.268800267197456
7	25.741546771305142	33.771154778410995	21.120474067517687	19.36682438276618
8	27.877837023277248	33.82574641322561	18.67842906695721	19.61798749653993
9	24.48244530594088	14.382452148092442	21.988741021235832	39.14636152473084
10	16.359917137177234	27.874197580956274	32.92994447874763	22.835940803118856
11	32.83332248432908	22.48129274654986	23.912862184301776	20.772522584819285
12	21.753944999499836	27.577364665257615	30.908182277413264	19.76050805782928
13	32.314837132876455	20.639318995871626	26.538335077839353	20.50750879341257
14	20.4961121397446	22.62678725213578	28.936561192855297	27.940539415264322
15	22.28330708428886	29.731581770262178	26.108839290337865	21.8762718551111
16	21.365647699071776	28.23772587678845	26.847770862375214	23.548855561764558
17	21.48107001267982	26.541059460376772	27.379774142448603	24.59809638449481
18	23.14545977490777	24.93375695055495	30.447200114632032	21.47358315990524
19	22.66998223328243	23.89377070972661	32.14758916061774	21.288657896373223
20	23.59866392952875	23.7755408263281	31.638379187880282	20.987416056262866
21	22.283639833301063	24.768193520170517	32.47378717664335	20.474379469885072
22	20.663651267388996	25.32425871318886	31.900065489164962	22.11202453025718
23	22.021350424431763	25.37947425240136	32.71661076829876	19.88256455486813
24	21.588007226476673	25.506813140008934	30.275813576534038	22.629366056980356
25	21.707713683616834	25.65636302418108	31.556959663956775	21.07896362824532
26	21.30304929115102	26.69595412555743	31.440871852324324	20.560124730967225
27	19.950778102369817	26.79367835107891	31.21978093052845	22.035762616022822
28	20.75133063210458	26.123792199073755	31.202977105412184	21.92190006340948
29	21.075220201858027	26.171208933312727	30.320173179223403	22.43339768560584
30	20.91799629359194	26.503770774196727	32.25186438231697	20.326368549894365
31	22.06853839372485	26.225551006368192	31.841148117191707	19.86476248271525
32	21.447129613435074	26.061859289177402	30.668706972693162	21.822304124694362
33	20.945718445671133	26.08278088331969	31.46570324736	21.505797423649177
34	20.16887428305586	26.858002894500473	31.949312342971066	21.02381047947261
35	19.57319116077205	29.359256422419875	30.54284465882724	20.524707757980828
36	22.170006045633617	26.62501619551833	30.42590417785102	20.779073580997036
37	21.09524753303002	27.556630242434693	30.384830471657164	20.963291752878124
38	20.792196370165808	25.928967652428682	31.725185086438835	21.553650890966676
39	20.396037874324442	25.677409399202944	32.47609562291551	21.450457103557106
40	19.049693777323114	26.347815471539665	32.11974222765897	22.48274852347825
41	20.681598917234716	25.278776082583317	32.19502669166998	21.84459830851199
42	19.27781402200178	27.531986018718378	31.351487148921198	21.838712810358643
43	19.596545982066075	27.349327607831995	30.599827927167066	22.454298482934863
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	820.0
1	997.0
2	1174.0
3	3235.5
4	5297.0
5	5297.0
6	8133.0
7	10969.0
8	11678.5
9	12388.0
10	18544.0
11	24700.0
12	24700.0
13	45661.0
14	66622.0
15	113223.0
16	159824.0
17	144822.5
18	129821.0
19	129821.0
20	129358.5
21	128896.0
22	97537.5
23	66179.0
24	73192.5
25	80206.0
26	80206.0
27	89129.5
28	98053.0
29	105572.0
30	113091.0
31	120875.5
32	128660.0
33	128660.0
34	144060.0
35	159460.0
36	176054.5
37	192649.0
38	205301.0
39	217953.0
40	217953.0
41	230895.5
42	243838.0
43	261232.5
44	278627.0
45	300454.0
46	322281.0
47	322281.0
48	431580.0
49	540879.0
50	502532.5
51	464186.0
52	416974.0
53	369762.0
54	369762.0
55	324845.5
56	279929.0
57	235684.5
58	191440.0
59	170793.0
60	150146.0
61	150146.0
62	132659.5
63	115173.0
64	100148.0
65	85123.0
66	72075.0
67	59027.0
68	59027.0
69	50535.5
70	42044.0
71	35120.5
72	28197.0
73	22929.0
74	17661.0
75	17661.0
76	14120.5
77	10580.0
78	8263.5
79	5947.0
80	4657.5
81	3368.0
82	3368.0
83	2532.0
84	1696.0
85	1279.5
86	863.0
87	674.0
88	485.0
89	485.0
90	373.5
91	262.0
92	184.5
93	107.0
94	69.5
95	32.0
96	32.0
97	20.5
98	9.0
99	7.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4808429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.768234692781355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.28559026721703	28.056356075040473
2	12.965608402961712	9.79376282195512
3	4.852225535704878	5.497799784444258
4	2.3816651546109866	3.5980515407586857
5	1.3197778805373075	2.492284036723729
6	0.778461564011783	1.7640671429343995
7	0.5460686999423846	1.4436835572464188
8	0.4048675152728185	1.2232905069045639
9	0.29316827699479586	0.9965203461016003
>10	1.8119229130315895	13.299711400222597
>50	0.20217803949822197	5.320792721291383
>100	0.14025940014725338	9.886402207925485
>500	0.01084633627580772	2.7915978165327444
>1k	0.00603189100358393	4.827229710131025
>5k	7.747382940383025E-4	1.967663969537613
>10k+	5.533844957416445E-4	7.040786362249868
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	87714	1.824171678525356	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	64166	1.334448319815058	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	64151	1.3341363676161173	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27426	0.570373400543088	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	18181	0.37810686192933285	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	17831	0.3708279772873843	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	16759	0.34853379346975905	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	13978	0.29069785578616214	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	13750	0.2859561823622643	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	12918	0.26865323372768946	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	9863	0.20511896921011	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	9101	0.18927179750392487	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	8479	0.17633617965451917	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7928	0.16487713554676592	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7926	0.16483554192024047	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	6305	0.13112390762138737	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6221	0.1293769753073197	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	6063	0.1260910788118115	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6020	0.12519681584151496	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	5413	0.11257315019104994	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5388	0.11205322985948217	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	5207	0.10828900665893162	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	5180	0.10772749270083845	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5051	0.10504470378994886	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.0796813262710128E-5	0.0	0.0	0.0	0.0
9	4.1593626525420255E-5	0.0	0.0	2.0796813262710128E-5	0.0
10	8.318725305084051E-5	0.0	0.0	8.318725305084051E-5	0.0
11	8.318725305084051E-5	0.0	0.0	2.4956175915252153E-4	0.0
12	8.318725305084051E-5	0.0	0.0	4.1593626525420255E-4	0.0
13	8.318725305084051E-5	0.0	0.0	4.367330785169127E-4	0.0
14	1.0398406631355064E-4	0.0	0.0	4.7832670504233295E-4	0.0
15	1.0398406631355064E-4	0.0	0.0	6.44701211144014E-4	0.0
16	1.2478087957626077E-4	0.0	0.0	0.0010814342896609266	0.0
17	1.2478087957626077E-4	0.0	0.0	0.0017053386875422304	0.0
18	1.2478087957626077E-4	0.0	0.0	0.0020796813262710127	0.0
19	1.6637450610168102E-4	0.0	0.0	0.0032858964955082004	0.0
20	1.6637450610168102E-4	0.0	0.0	0.0055735459544063145	0.0
21	1.8717131936439116E-4	0.0	0.0	0.011001514215973658	8.318725305084051E-5
22	1.8717131936439116E-4	0.0	0.0	0.018384382924235755	8.318725305084051E-5
23	2.0796813262710128E-4	0.0	0.0	0.022502151950252357	8.318725305084051E-5
24	2.0796813262710128E-4	0.0	0.0	0.03028016011050595	8.318725305084051E-5
25	2.2876494588981142E-4	0.0	0.0	0.03527139529355638	8.318725305084051E-5
26	2.2876494588981142E-4	0.0	0.0	0.04423482180978444	8.318725305084051E-5
27	2.2876494588981142E-4	0.0	0.0	0.08308326898452696	8.318725305084051E-5
28	2.2876494588981142E-4	0.0	0.0	0.18814876958773852	8.318725305084051E-5
29	2.4956175915252153E-4	0.0	0.0	0.3468492515954795	8.318725305084051E-5
30	3.119521989406519E-4	0.0	0.0	0.5604117269902499	8.318725305084051E-5
31	3.119521989406519E-4	0.0	0.0	0.8428948415376415	8.318725305084051E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	2025	0.0	21.560492	1
GGTATCA	22805	0.0	21.270336	1
TATAGCG	50	0.00703806	18.5	5
ATTGGAC	2380	0.0	18.189075	3
TTGGACC	3095	0.0	17.573507	4
TGGACCC	3220	0.0	16.48913	5
GGACCCT	3155	0.0	16.47702	6
TATTGGA	2585	0.0	16.245647	2
ACCGTTA	285	0.0	15.578948	8
CGGTATA	205	5.456968E-12	15.341463	28
CGTTTAT	300	0.0	14.8	1
GACCCTC	3590	0.0	14.171309	7
TTTTTAC	3435	0.0	14.056768	1
GACGTAT	265	0.0	13.962264	28
ATCGTAC	120	3.304657E-5	13.874999	25
TCTATAC	375	0.0	13.813334	3
TCTAACG	255	1.8189894E-12	13.784313	2
GTATCAA	35160	0.0	13.775029	2
CTCTAAT	730	0.0	13.684932	1
GATACTG	1985	0.0	13.513854	36
>>END_MODULE