FastQCFastQC Report
Wed 25 May 2016
SRR2088557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4575011
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT872521.9071429555032764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT617331.3493519469133517No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT611061.335647061832201No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265110.5794740165652061No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG135590.29637087211375013No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA134330.2936167803749543No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG120160.2626441772489727No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA112280.24542017494602747No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG110190.24085187991897727No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA101760.22242569471417664No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA87100.19038205591199672No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT77760.16996680445139914No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC74550.16295042787875264No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA69370.15162805073036983No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA59060.1290925857883183No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58860.12865542836946184No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT50590.11057896909974643No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA47760.10439319162292725No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46100.10076478504641848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA231700.019.609841
GTATTGG18650.018.747991
ATTGGAC20150.017.2605463
TTGGACC27000.016.8555564
GGACCCT25400.016.8248026
TGGACCC26300.016.8117875
ACGGACC1352.2240783E-816.44444527
TATACCG2700.015.7592595
TAATACT9850.015.5888334
CTCTAAT8000.015.0312511
GACCCTC28500.014.7999997
TCGGGTA2900.014.67241425
TAGTACT7350.014.3469384
TACTGGT9200.014.2771747
TATACAC23850.014.27253737
AACGGAC1304.450647E-614.2307726
TATTGGA24600.014.2134142
CTAATAC10350.014.1207733
TTATGCG3700.014.04
CGACGAA5550.013.66666810
Produced by FastQC (version 0.11.5)