##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088557_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4575011
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79924288706628	31.0	31.0	34.0	30.0	34.0
2	31.941043857599468	31.0	31.0	34.0	30.0	34.0
3	32.06344050320316	33.0	31.0	34.0	30.0	34.0
4	35.70733250696009	37.0	35.0	37.0	35.0	37.0
5	35.64552850255442	37.0	35.0	37.0	33.0	37.0
6	35.71071566822462	37.0	35.0	37.0	33.0	37.0
7	35.56302247142138	37.0	35.0	37.0	33.0	37.0
8	35.611997872792	37.0	35.0	37.0	33.0	37.0
9	37.222807333140835	39.0	37.0	39.0	34.0	39.0
10	37.09046950925364	39.0	37.0	39.0	33.0	39.0
11	37.15730344692067	39.0	37.0	39.0	33.0	39.0
12	37.01299384853938	39.0	37.0	39.0	33.0	39.0
13	37.06911546223605	39.0	37.0	39.0	33.0	39.0
14	38.08532831942918	40.0	37.0	41.0	33.0	41.0
15	38.09453704045739	40.0	37.0	41.0	33.0	41.0
16	37.99012832974609	40.0	37.0	41.0	33.0	41.0
17	38.04770524049013	40.0	37.0	41.0	33.0	41.0
18	38.0289931543334	40.0	37.0	41.0	33.0	41.0
19	38.0984391075781	40.0	37.0	41.0	33.0	41.0
20	38.01180565467493	40.0	37.0	41.0	33.0	41.0
21	37.87887329669808	40.0	37.0	41.0	32.0	41.0
22	37.76125631173346	40.0	37.0	41.0	32.0	41.0
23	37.585685804908444	40.0	36.0	41.0	32.0	41.0
24	37.73113856994005	40.0	37.0	41.0	32.0	41.0
25	37.67183029723863	40.0	37.0	41.0	32.0	41.0
26	37.47116979609448	39.0	36.0	41.0	32.0	41.0
27	37.431343225185685	39.0	36.0	41.0	32.0	41.0
28	37.28173003299883	39.0	36.0	41.0	31.0	41.0
29	37.06536115432291	39.0	36.0	41.0	31.0	41.0
30	36.89675871817576	39.0	35.0	41.0	31.0	41.0
31	36.613254044635084	39.0	35.0	40.0	30.0	41.0
32	36.445304940250416	38.0	35.0	40.0	30.0	41.0
33	36.26034910079998	38.0	35.0	40.0	30.0	41.0
34	36.05581254340154	38.0	35.0	40.0	30.0	41.0
35	35.83531689869161	38.0	35.0	40.0	29.0	41.0
36	35.738878223462194	38.0	35.0	40.0	27.0	41.0
37	35.5726069292511	38.0	35.0	40.0	27.0	41.0
38	35.419096260096424	38.0	35.0	40.0	26.0	41.0
39	35.26431433716771	38.0	34.0	40.0	25.0	41.0
40	35.05629647666421	38.0	34.0	40.0	24.0	41.0
41	34.87763089531369	38.0	34.0	40.0	23.0	41.0
42	34.56121438833699	38.0	33.0	40.0	22.0	41.0
43	34.15883546509506	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	3.0
12	7.0
13	13.0
14	17.0
15	28.0
16	52.0
17	122.0
18	279.0
19	664.0
20	1446.0
21	2796.0
22	5259.0
23	9122.0
24	15126.0
25	23260.0
26	34705.0
27	49442.0
28	67672.0
29	88886.0
30	112542.0
31	138824.0
32	171072.0
33	212083.0
34	267509.0
35	333241.0
36	445587.0
37	672714.0
38	864291.0
39	1058245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.428663013050674	20.69706499066341	14.504030700691212	22.3702412955947
2	18.343300158185414	24.084860123833582	35.5444391281245	22.027400589856505
3	19.49175641326327	24.55183167865607	33.58149302810419	22.374918879976462
4	12.927772195520404	16.03185216385272	36.65752934801687	34.38284629261001
5	12.253456876934285	39.19535931170439	34.65703142571679	13.894152385644537
6	31.661606933841252	38.00137311145263	13.399552481950316	16.937467472755806
7	25.478146391342012	34.03937170861447	21.216473577877736	19.266008322165785
8	27.938796212730416	33.925557774615186	18.73425003786876	19.401395974785636
9	24.64262927455256	14.459615506935394	22.01745525857752	38.880299959934526
10	16.63766491490403	28.098205665516435	32.56223864816937	22.701890771410167
11	32.852336311322524	22.937627909528523	23.543724812902088	20.666310966246858
12	21.63409006011133	27.954971037228106	30.704647486093478	19.706291416567087
13	32.161299721465156	20.875578222653456	26.30596953755958	20.657152518321816
14	20.37693461283481	23.05657844319937	29.022465738333743	27.544021205632074
15	22.554437574029876	30.009720195208274	25.961183481307476	21.474658749454374
16	21.170156749349893	28.31105761275765	27.02609895364186	23.492686684250597
17	21.296211091077158	26.979913272339672	27.641310589198582	24.082565047384584
18	22.612645084350618	25.341141256272387	30.53972984982987	21.506483809547124
19	22.381891540807224	24.639853324942827	32.21218484502004	20.76607028922991
20	23.3105231878131	24.408028745723236	31.494372363257707	20.78707570320596
21	21.943597512661718	25.352572922775487	32.28689504790262	20.416934516660177
22	20.639032343310213	25.97475721916297	31.70827348830418	21.677936949222637
23	21.734832987286808	25.976768143289707	32.332075267141434	19.95632360228205
24	21.310134554867737	26.203456122837736	30.348823205015247	22.13758611727928
25	21.42539110834925	26.12614483331297	31.543115415460203	20.90534864287758
26	20.87997165471296	27.28019670335219	31.460623810521987	20.379207831412867
27	19.895711726157597	27.202710550859877	31.175793894266047	21.725783828716477
28	20.4779398344616	26.86944359259464	31.15483219603188	21.49778437691188
29	20.727731583596192	26.941552708835015	30.402855861985906	21.927859845582883
30	20.5904860119462	27.2541202633174	32.11806485274025	20.037328871996156
31	21.48276801957416	26.9705362457052	31.746502904583178	19.80019283013746
32	20.852015437777087	26.855716849642548	30.76047248848145	21.531795224098914
33	20.435251412510265	26.83005570915567	31.519443341229124	21.21524953710494
34	19.81315454760655	27.330098222714653	32.00744216789862	20.84930506178018
35	19.366882396566915	29.49147007515392	30.791904106897228	20.349743421381937
36	21.615226717487673	27.24824049603378	30.57395927572633	20.562573510752213
37	20.56060630236736	27.887670652595155	30.588844485838397	20.96287855919909
38	20.27437748237108	26.468395376535707	31.85813105148818	21.39909608960503
39	19.778007091130494	26.30559795375355	32.58916754517093	21.327227409945028
40	18.695955047976934	26.713902982965504	32.41970347175121	22.170438497306343
41	19.988979261470625	25.681883606400074	32.60171396309211	21.727423169037188
42	18.916850691725113	27.54942009975495	31.72847016105535	21.805259047464585
43	18.94646810685264	27.283191231671356	31.311028541789298	22.459312119686707
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1130.0
1	1171.0
2	1212.0
3	3400.0
4	5588.0
5	5588.0
6	8519.5
7	11451.0
8	12175.0
9	12899.0
10	19241.5
11	25584.0
12	25584.0
13	46366.0
14	67148.0
15	113964.0
16	160780.0
17	144350.5
18	127921.0
19	127921.0
20	127908.5
21	127896.0
22	97796.0
23	67696.0
24	74754.5
25	81813.0
26	81813.0
27	89694.0
28	97575.0
29	103882.0
30	110189.0
31	119178.0
32	128167.0
33	128167.0
34	142535.0
35	156903.0
36	174386.0
37	191869.0
38	202855.5
39	213842.0
40	213842.0
41	223124.0
42	232406.0
43	250891.5
44	269377.0
45	287658.0
46	305939.0
47	305939.0
48	395772.0
49	485605.0
50	454826.5
51	424048.0
52	382264.0
53	340480.0
54	340480.0
55	301349.5
56	262219.0
57	223176.0
58	184133.0
59	163434.5
60	142736.0
61	142736.0
62	125165.5
63	107595.0
64	92495.0
65	77395.0
66	65360.5
67	53326.0
68	53326.0
69	45622.5
70	37919.0
71	31757.0
72	25595.0
73	20715.5
74	15836.0
75	15836.0
76	12579.5
77	9323.0
78	7310.0
79	5297.0
80	4101.0
81	2905.0
82	2905.0
83	2197.0
84	1489.0
85	1142.5
86	796.0
87	624.0
88	452.0
89	452.0
90	376.5
91	301.0
92	202.0
93	103.0
94	76.5
95	50.0
96	50.0
97	31.0
98	12.0
99	11.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4575011.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.65097492753248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63831845719959	28.848437753224054
2	12.865364207860452	9.94517738863176
3	4.71628600974973	5.46867156941727
4	2.2991763579897246	3.554616310665451
5	1.264992529477209	2.4446597270169743
6	0.7985924936150536	1.8519827068818644
7	0.5391033563468185	1.4585809216656642
8	0.39216634965527897	1.2126089398358497
9	0.28846437528526325	1.0034486402973326
>10	1.8573273423298045	13.891974457400465
>50	0.19063029165506665	5.1221508511179366
>100	0.13119743916663212	9.46707140007475
>500	0.011175974006499988	2.8952656228768463
>1k	0.006240391519012356	5.034553346515592
>5k	3.9711582393714987E-4	1.046554720828565
>10k+	5.673083199102141E-4	6.754245643549736
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	87252	1.9071429555032764	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	61733	1.3493519469133517	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	61106	1.335647061832201	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26511	0.5794740165652061	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	13559	0.29637087211375013	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	13433	0.2936167803749543	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12016	0.2626441772489727	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	11228	0.24542017494602747	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	11019	0.24085187991897727	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10176	0.22242569471417664	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	8710	0.19038205591199672	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7776	0.16996680445139914	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	7455	0.16295042787875264	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6937	0.15162805073036983	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5906	0.1290925857883183	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5886	0.12865542836946184	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	5059	0.11057896909974643	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4776	0.10439319162292725	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4610	0.10076478504641848	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.1857870942823962E-5
2	2.1857870942823962E-5	0.0	0.0	0.0	2.1857870942823962E-5
3	2.1857870942823962E-5	0.0	0.0	0.0	2.1857870942823962E-5
4	2.1857870942823962E-5	0.0	0.0	0.0	2.1857870942823962E-5
5	2.1857870942823962E-5	0.0	0.0	0.0	2.1857870942823962E-5
6	4.3715741885647923E-5	0.0	0.0	0.0	2.1857870942823962E-5
7	4.3715741885647923E-5	0.0	0.0	2.1857870942823962E-5	2.1857870942823962E-5
8	4.3715741885647923E-5	0.0	0.0	4.3715741885647923E-5	2.1857870942823962E-5
9	6.557361282847189E-5	0.0	0.0	1.0928935471411982E-4	2.1857870942823962E-5
10	8.743148377129585E-5	0.0	0.0	1.748629675425917E-4	2.1857870942823962E-5
11	8.743148377129585E-5	0.0	0.0	2.1857870942823963E-4	2.1857870942823962E-5
12	8.743148377129585E-5	0.0	0.0	5.464467735705991E-4	2.1857870942823962E-5
13	8.743148377129585E-5	0.0	0.0	5.68304644513423E-4	2.1857870942823962E-5
14	8.743148377129585E-5	0.0	0.0	7.213097411131907E-4	2.1857870942823962E-5
15	8.743148377129585E-5	0.0	0.0	9.836041924270783E-4	2.1857870942823962E-5
16	8.743148377129585E-5	0.0	0.0	0.0014207616112835576	2.1857870942823962E-5
17	1.0928935471411982E-4	0.0	0.0	0.0020546398686254524	2.1857870942823962E-5
18	1.3114722565694378E-4	0.0	0.0	0.0025355130293675796	2.1857870942823962E-5
19	1.3114722565694378E-4	0.0	0.0	0.0032786806414235943	2.1857870942823962E-5
20	1.3114722565694378E-4	0.0	0.0	0.00590162515456247	2.1857870942823962E-5
21	1.5300509659976774E-4	0.0	0.0	0.010775930374812214	2.1857870942823962E-5
22	1.748629675425917E-4	0.0	0.0	0.018775911139885783	4.3715741885647923E-5
23	1.748629675425917E-4	0.0	0.0	0.022907048748079514	4.3715741885647923E-5
24	1.748629675425917E-4	0.0	0.0	0.029639272998469295	1.3114722565694378E-4
25	1.748629675425917E-4	0.0	0.0	0.03468844118626163	1.3114722565694378E-4
26	1.748629675425917E-4	0.0	0.0	0.042732137693220845	1.3114722565694378E-4
27	1.748629675425917E-4	0.0	0.0	0.08789049906109515	1.3114722565694378E-4
28	1.9672083848541568E-4	0.0	0.0	0.2033219155101485	1.3114722565694378E-4
29	1.9672083848541568E-4	0.0	0.0	0.3835400614337321	1.3114722565694378E-4
30	1.9672083848541568E-4	0.0	0.0	0.614009452654868	1.3114722565694378E-4
31	1.9672083848541568E-4	0.0	0.0	0.9340086832578107	1.3114722565694378E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23170	0.0	19.60984	1
GTATTGG	1865	0.0	18.74799	1
ATTGGAC	2015	0.0	17.260546	3
TTGGACC	2700	0.0	16.855556	4
GGACCCT	2540	0.0	16.824802	6
TGGACCC	2630	0.0	16.811787	5
ACGGACC	135	2.2240783E-8	16.444445	27
TATACCG	270	0.0	15.759259	5
TAATACT	985	0.0	15.588833	4
CTCTAAT	800	0.0	15.031251	1
GACCCTC	2850	0.0	14.799999	7
TCGGGTA	290	0.0	14.672414	25
TAGTACT	735	0.0	14.346938	4
TACTGGT	920	0.0	14.277174	7
TATACAC	2385	0.0	14.272537	37
AACGGAC	130	4.450647E-6	14.23077	26
TATTGGA	2460	0.0	14.213414	2
CTAATAC	1035	0.0	14.120773	3
TTATGCG	370	0.0	14.0	4
CGACGAA	555	0.0	13.666668	10
>>END_MODULE