FastQCFastQC Report
Wed 25 May 2016
SRR2088556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195322
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT144331.2074570701451157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105310.881017834524923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98430.8234601220424288No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45580.38131984519652445No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG22470.18798281969209968No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA19090.15970592024575805No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG18480.15460269283088574No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG18410.1540170765701627No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA17870.14949946541601342No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16420.13736884287246448No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13690.11452980870426546No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA12120.10139527257090558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG4000.021.2750021
GGTATCA38100.021.1706031
GTCTAGA759.266283E-619.7333321
ATTGGAC3800.019.4736843
CTAGACT1258.578354E-917.764
GGACCCT5300.017.1037736
TTGGACC5550.017.04
TATTGGA4750.016.7473682
CTAATAC2000.016.6500023
TGGACCC5500.016.4818175
TCTACAC1951.8189894E-1216.1282063
TTGCGAT1951.8189894E-1216.12820611
TGCGATA1851.8189894E-1116.012
AGTGCGC700.00259257515.8571428
GACCCTC5550.015.6666677
CCTATAA1302.589004E-715.6538461
GGACCGT957.06005E-515.5789486
GCGTGCG1353.9722363E-715.0740739
TAGAACT1601.0963049E-815.0312514
CCCTCGC5600.014.8660719
Produced by FastQC (version 0.11.5)