##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088556_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1195322
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.78035123590129	31.0	31.0	34.0	30.0	34.0
2	31.94189515461106	31.0	31.0	34.0	30.0	34.0
3	32.0797324904921	33.0	31.0	34.0	30.0	34.0
4	35.737402975934515	37.0	35.0	37.0	35.0	37.0
5	35.6400944682688	37.0	35.0	37.0	33.0	37.0
6	35.689792373937735	37.0	35.0	37.0	33.0	37.0
7	35.55727243370406	37.0	35.0	37.0	33.0	37.0
8	35.584399852090066	37.0	35.0	37.0	33.0	37.0
9	37.214384910509466	39.0	37.0	39.0	33.0	39.0
10	37.06008757472882	39.0	37.0	39.0	33.0	39.0
11	37.12728787724144	39.0	37.0	39.0	33.0	39.0
12	36.989220477829406	39.0	37.0	39.0	33.0	39.0
13	37.039870428219345	39.0	37.0	39.0	33.0	39.0
14	38.125889090972976	40.0	37.0	41.0	33.0	41.0
15	38.126211179916375	40.0	37.0	41.0	33.0	41.0
16	38.027020334269764	40.0	37.0	41.0	33.0	41.0
17	38.01576478973866	40.0	37.0	41.0	33.0	41.0
18	37.97629258057661	40.0	37.0	41.0	33.0	41.0
19	38.02475232615145	40.0	37.0	41.0	33.0	41.0
20	37.92884846091681	40.0	37.0	41.0	33.0	41.0
21	37.78716111641884	40.0	37.0	41.0	32.0	41.0
22	37.66535879035105	40.0	37.0	41.0	32.0	41.0
23	37.50240186326362	39.0	36.0	41.0	32.0	41.0
24	37.67542135089959	40.0	37.0	41.0	32.0	41.0
25	37.62710298982199	40.0	37.0	41.0	32.0	41.0
26	37.45165319470402	39.0	36.0	41.0	32.0	41.0
27	37.406376691803544	39.0	36.0	41.0	32.0	41.0
28	37.28160027172594	39.0	36.0	41.0	31.0	41.0
29	37.08621275271433	39.0	36.0	41.0	31.0	41.0
30	36.981026033152574	39.0	36.0	41.0	31.0	41.0
31	36.76960601411168	39.0	35.0	41.0	30.0	41.0
32	36.61673674541253	39.0	35.0	40.0	30.0	41.0
33	36.47165533638635	39.0	35.0	40.0	30.0	41.0
34	36.2954249984523	39.0	35.0	40.0	30.0	41.0
35	36.11854128009022	38.0	35.0	40.0	29.0	41.0
36	36.080048723272895	38.0	35.0	40.0	29.0	41.0
37	35.96510312702351	38.0	35.0	40.0	28.0	41.0
38	35.8522624029341	38.0	35.0	40.0	27.0	41.0
39	35.7449222887222	38.0	35.0	40.0	27.0	41.0
40	35.59897835060344	38.0	35.0	40.0	26.0	41.0
41	35.48930915686317	38.0	35.0	40.0	26.0	41.0
42	35.259472343017194	38.0	34.0	40.0	24.0	41.0
43	34.90057323465978	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	2.0
14	1.0
15	4.0
16	17.0
17	36.0
18	70.0
19	160.0
20	337.0
21	685.0
22	1327.0
23	2334.0
24	3671.0
25	5647.0
26	8336.0
27	12086.0
28	16467.0
29	21813.0
30	28056.0
31	34967.0
32	43500.0
33	53914.0
34	68091.0
35	85786.0
36	114006.0
37	165167.0
38	236163.0
39	292676.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.431513851497755	20.958034738756588	13.933985988712665	23.676465421032994
2	18.138543421772543	23.214163212925055	35.70167703765178	22.945616327650626
3	19.064402729975686	24.68238683802356	32.46087665081041	23.79233378119034
4	13.648121594013999	16.45037906103962	36.60871296604597	33.292786378900416
5	12.838465283831468	38.47933862172703	34.978273636727174	13.703922457714324
6	32.118291138287425	37.98156480011244	14.33847950594066	15.56166455565948
7	26.20164273727079	33.02925906157504	21.969561339956932	18.799536861197232
8	26.90287637975374	34.9917428107238	19.433759271560298	18.671621537962153
9	25.07667390042181	14.612882553822319	21.356588433911533	38.95385511184434
10	16.162004882366425	28.009105496259583	33.5344785756474	22.29441104572659
11	33.66624223431008	22.830584562151454	23.259422983932364	20.243750219606095
12	20.933020558477132	27.209070024646074	31.25358689959693	20.604322517279865
13	31.013986189495384	21.13689867667457	25.92590113793605	21.923213995893995
14	20.573786812256447	22.29859401901747	28.193992915716436	28.93362625300965
15	22.94854440895424	29.6720046983156	24.969506124709493	22.40994476802067
16	22.01791651119949	28.224695939671484	26.201224439941704	23.556163109187313
17	21.677589804253582	27.218523544283464	27.414119375364965	23.68976727609799
18	22.73671864150413	26.14299745173267	29.136667776548915	21.983616130214283
19	22.98518725498234	25.450297074763117	30.16308576266479	21.40142990758975
20	23.43845424078198	25.40863466078596	29.64699051803614	21.50592058039591
21	22.48247752488451	26.108948049144914	29.94875021124015	21.459824214730425
22	21.752883323489403	26.318598670483766	29.75608246146227	22.17243554456456
23	22.22873836505979	26.3582532572813	30.148110718283444	21.264897659375464
24	22.224471732303094	26.34177234251524	28.971774969422466	22.461980955759202
25	22.20297124958798	26.310316383367827	29.728893135071555	21.75781923197264
26	21.915182687175506	27.04392623912218	29.533548282387507	21.50734279131481
27	21.21947056943652	27.03965960636548	29.44152286998817	22.299346954209827
28	21.57502329916123	26.77328786720231	29.488288511380194	22.163400322256262
29	21.69415437848546	26.732545707349146	29.172390368453023	22.40090954571237
30	21.69256484863493	27.030875362454637	29.87111422696144	21.405445561948998
31	22.160304922021012	26.710961565168212	29.840494862472205	21.28823865033857
32	21.66177816521406	26.747269773333045	29.305241600171332	22.285710461281564
33	21.65040047786287	26.484913688529115	29.82878253725774	22.035903296350273
34	21.19981059496939	26.641858846402894	30.288323982993703	21.87000657563401
35	20.903572426509342	27.86721904223297	29.521083021980687	21.708125509277
36	22.240199711876798	26.53176298938696	29.398689223489573	21.82934807524667
37	21.407788026991888	26.801313788251203	29.524011103284302	22.266887081472607
38	21.160908943364216	26.077241111600053	30.485007387130832	22.2768425579049
39	20.896294052983215	25.761510287604512	31.009719556738684	22.33247610267359
40	20.34949578439952	26.039678011447958	30.89117409367518	22.719652110477345
41	20.916288665313616	25.42678876486838	31.0050346266529	22.651887943165107
42	20.11407804758885	26.66026392888276	30.477561694673067	22.748096328855322
43	20.213800130843403	26.295424998452297	30.328396867120322	23.16237800358397
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	356.0
1	336.5
2	317.0
3	726.0
4	1135.0
5	1135.0
6	1712.5
7	2290.0
8	2365.0
9	2440.0
10	3541.5
11	4643.0
12	4643.0
13	8379.5
14	12116.0
15	19816.5
16	27517.0
17	25369.0
18	23221.0
19	23221.0
20	24197.0
21	25173.0
22	20959.0
23	16745.0
24	18901.0
25	21057.0
26	21057.0
27	24068.5
28	27080.0
29	29446.0
30	31812.0
31	34764.5
32	37717.0
33	37717.0
34	42091.0
35	46465.0
36	50781.5
37	55098.0
38	58978.5
39	62859.0
40	62859.0
41	65297.0
42	67735.0
43	71516.0
44	75297.0
45	79090.0
46	82883.0
47	82883.0
48	96736.0
49	110589.0
50	104307.0
51	98025.0
52	91254.5
53	84484.0
54	84484.0
55	77144.0
56	69804.0
57	62377.5
58	54951.0
59	49870.5
60	44790.0
61	44790.0
62	39909.0
63	35028.0
64	29939.0
65	24850.0
66	20982.0
67	17114.0
68	17114.0
69	14560.5
70	12007.0
71	10063.5
72	8120.0
73	6614.0
74	5108.0
75	5108.0
76	4060.0
77	3012.0
78	2314.5
79	1617.0
80	1256.5
81	896.0
82	896.0
83	686.0
84	476.0
85	353.0
86	230.0
87	182.5
88	135.0
89	135.0
90	110.0
91	85.0
92	54.0
93	23.0
94	19.5
95	16.0
96	16.0
97	9.5
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1195322.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.531260790736226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.53227410401753	48.30718059234776
2	9.910296388769497	11.60124284889118
3	2.9759541629336357	5.225590476358373
4	1.3335184217058915	3.12210058040474
5	0.7475911212355213	2.1878725440937603
6	0.5062301171027379	1.7778172202559177
7	0.3480825990560649	1.4261599367447468
8	0.2382376775932576	1.1155481309912239
9	0.19449276891609454	1.0245516281406295
>10	1.0973710663612246	11.727820247841157
>50	0.07679907682601013	3.061097478777328
>100	0.03370270335634034	3.4783465903326274
>500	0.0034419739743984813	1.3025427314247942
>1k	0.0015775714049326374	1.7203267859650533
>5k	1.4341558226660338E-4	0.826250441800235
>10k+	2.8683116453320675E-4	2.095551765630506
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14433	1.2074570701451157	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10531	0.881017834524923	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9843	0.8234601220424288	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4558	0.38131984519652445	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2247	0.18798281969209968	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1909	0.15970592024575805	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1848	0.15460269283088574	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1841	0.1540170765701627	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1787	0.14949946541601342	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1642	0.13736884287246448	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1369	0.11452980870426546	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1212	0.10139527257090558	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	8.365946581757887E-5	0.0	0.0	0.0	0.0
9	8.365946581757887E-5	0.0	0.0	1.6731893163515773E-4	0.0
10	8.365946581757887E-5	0.0	0.0	2.509783974527366E-4	0.0
11	8.365946581757887E-5	0.0	0.0	4.182973290878943E-4	0.0
12	8.365946581757887E-5	0.0	0.0	4.182973290878943E-4	0.0
13	1.6731893163515773E-4	0.0	0.0	4.182973290878943E-4	0.0
14	1.6731893163515773E-4	0.0	0.0	4.182973290878943E-4	0.0
15	1.6731893163515773E-4	0.0	0.0	0.001171232521446104	0.0
16	1.6731893163515773E-4	0.0	0.0	0.001840508247986735	0.0
17	1.6731893163515773E-4	0.0	0.0	0.0025097839745273657	0.0
18	1.6731893163515773E-4	0.0	0.0	0.0028444218377976812	0.0
19	1.6731893163515773E-4	0.0	0.0	0.0034300380985207335	0.0
20	1.6731893163515773E-4	0.0	0.0	0.006860076197041467	0.0
21	1.6731893163515773E-4	0.0	0.0	0.012883557735907145	0.0
22	1.6731893163515773E-4	0.0	0.0	0.019492655535495874	0.0
23	1.6731893163515773E-4	0.0	0.0	0.024010266689645135	0.0
24	1.6731893163515773E-4	0.0	0.0	0.03220889433976786	0.0
25	1.6731893163515773E-4	0.0	0.0	0.036559186562281965	0.0
26	1.6731893163515773E-4	0.0	0.0	0.04684930085784416	0.0
27	2.509783974527366E-4	0.0	0.0	0.08800975804009296	0.0
28	2.509783974527366E-4	0.0	0.0	0.20655522110360222	0.0
29	2.509783974527366E-4	0.0	0.0	0.3628311032508395	0.0
30	2.509783974527366E-4	0.0	0.0	0.5655379889268332	0.0
31	2.509783974527366E-4	0.0	0.0	0.8990046196757024	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	400	0.0	21.275002	1
GGTATCA	3810	0.0	21.170603	1
GTCTAGA	75	9.266283E-6	19.733332	1
ATTGGAC	380	0.0	19.473684	3
CTAGACT	125	8.578354E-9	17.76	4
GGACCCT	530	0.0	17.103773	6
TTGGACC	555	0.0	17.0	4
TATTGGA	475	0.0	16.747368	2
CTAATAC	200	0.0	16.650002	3
TGGACCC	550	0.0	16.481817	5
TCTACAC	195	1.8189894E-12	16.128206	3
TTGCGAT	195	1.8189894E-12	16.128206	11
TGCGATA	185	1.8189894E-11	16.0	12
AGTGCGC	70	0.002592575	15.857142	8
GACCCTC	555	0.0	15.666667	7
CCTATAA	130	2.589004E-7	15.653846	1
GGACCGT	95	7.06005E-5	15.578948	6
GCGTGCG	135	3.9722363E-7	15.074073	9
TAGAACT	160	1.0963049E-8	15.031251	4
CCCTCGC	560	0.0	14.866071	9
>>END_MODULE