FastQCFastQC Report
Wed 25 May 2016
SRR2088555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836966
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT85961.027042914526994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61600.7359916651333507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT61350.7330046859729069No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26180.3127964576816741No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG14220.16989937464604296No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA12750.15233593718263347No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG12220.1460035413624926No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA12110.14468927053189737No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA11200.13381666638788195No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG11100.13262187472370443No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA8810.10526114561403928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19150.022.7989541
CGGGATA450.003824952520.55555524
TACCCCG851.2444798E-619.5882365
CTATTAT1103.8478902E-818.51
TAGGACC1002.8726026E-718.54
ACGTTCG752.0664203E-417.26666622
GTGTAGA1107.8013545E-716.8181821
CGTGCAA904.443634E-516.44444511
TAACCGT904.443634E-516.4444457
ATCGGCT700.002591704715.8571423
AACCGTG957.056013E-515.5789488
GCTTCGT957.056013E-515.57894828
ATTATAC1557.197741E-915.51612953
CGTTCGC1201.9338713E-615.41666723
TTACCCC1201.9338713E-615.4166674
CAGTTCG855.3619867E-415.23529413
TGATCGC750.00410316614.89
TTAGGAC1508.090683E-814.83
CGAACTA750.00410316614.829
CTACTCG750.00410316614.814
Produced by FastQC (version 0.11.5)