##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088555_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	836966
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.786753583777596	31.0	31.0	34.0	30.0	34.0
2	31.957580116755043	31.0	31.0	34.0	30.0	34.0
3	32.098240549795335	33.0	31.0	34.0	30.0	34.0
4	35.757162178630914	37.0	35.0	37.0	35.0	37.0
5	35.65305520176447	37.0	35.0	37.0	33.0	37.0
6	35.683968046491735	37.0	35.0	37.0	33.0	37.0
7	35.5589785009188	37.0	35.0	37.0	33.0	37.0
8	35.57563748109242	37.0	35.0	37.0	33.0	37.0
9	37.213500906846875	39.0	37.0	39.0	33.0	39.0
10	37.05713135300598	39.0	37.0	39.0	33.0	39.0
11	37.11431408205351	39.0	37.0	39.0	33.0	39.0
12	36.977038493797835	39.0	37.0	39.0	33.0	39.0
13	37.03350793222186	39.0	37.0	39.0	33.0	39.0
14	38.12060585495707	40.0	37.0	41.0	33.0	41.0
15	38.12165966120487	40.0	37.0	41.0	33.0	41.0
16	38.03056038118633	40.0	37.0	41.0	33.0	41.0
17	38.01175555518384	40.0	37.0	41.0	33.0	41.0
18	37.96453499903222	40.0	37.0	41.0	33.0	41.0
19	38.01554782392594	40.0	37.0	41.0	33.0	41.0
20	37.90470580644853	40.0	37.0	41.0	32.0	41.0
21	37.7720480879749	40.0	37.0	41.0	32.0	41.0
22	37.636650712215314	40.0	37.0	41.0	32.0	41.0
23	37.4829933354521	39.0	36.0	41.0	32.0	41.0
24	37.65014827364553	40.0	37.0	41.0	32.0	41.0
25	37.60724927894323	40.0	37.0	41.0	32.0	41.0
26	37.416805461631654	39.0	36.0	41.0	32.0	41.0
27	37.39392878563765	39.0	36.0	41.0	32.0	41.0
28	37.25679776717334	39.0	36.0	41.0	31.0	41.0
29	37.06065120924865	39.0	36.0	41.0	31.0	41.0
30	36.97080048651917	39.0	36.0	41.0	31.0	41.0
31	36.776350532757604	39.0	35.0	41.0	30.0	41.0
32	36.64042864345744	39.0	35.0	41.0	30.0	41.0
33	36.495422753134534	39.0	35.0	40.0	30.0	41.0
34	36.32696668681882	39.0	35.0	40.0	30.0	41.0
35	36.16263265174452	38.0	35.0	40.0	29.0	41.0
36	36.13645118200739	39.0	35.0	40.0	29.0	41.0
37	36.035353885343014	39.0	35.0	40.0	28.0	41.0
38	35.91880434808582	38.0	35.0	40.0	27.0	41.0
39	35.83546643471778	39.0	35.0	40.0	27.0	41.0
40	35.69673200584014	38.0	35.0	40.0	27.0	41.0
41	35.62243388620326	38.0	35.0	40.0	26.0	41.0
42	35.386909384610604	38.0	34.0	40.0	25.0	41.0
43	35.04192643428765	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	8.0
16	4.0
17	24.0
18	41.0
19	100.0
20	273.0
21	493.0
22	889.0
23	1523.0
24	2604.0
25	4100.0
26	5992.0
27	8560.0
28	11719.0
29	15236.0
30	19315.0
31	24575.0
32	30325.0
33	38014.0
34	47349.0
35	59506.0
36	78410.0
37	112755.0
38	163093.0
39	212057.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.14217303928714	21.170752455894267	13.63651570075726	24.050558804061335
2	17.925578816821712	23.02160422287166	36.04662554990286	23.00619141040377
3	18.712827044348277	24.62907692785609	32.64421732782455	24.013878699971087
4	13.390269138770272	16.47904454900199	37.21847721412817	32.91220909809957
5	12.445905807404364	38.67349450276355	35.583404821701244	13.297194868130843
6	31.891259621059874	38.55043096135327	14.665111844447685	14.893197573139172
7	26.07298265401462	33.255353264051344	22.173780057971292	18.49788402396274
8	26.46774181985887	35.19892086416891	19.89519287521835	18.438144440753863
9	25.026823072860786	14.67216111526633	21.466463392778202	38.83455241909468
10	15.699442988126162	28.00388546249191	34.27391315776268	22.022758391619256
11	33.616658263298625	22.940597348040423	23.43882547200245	20.003918916658503
12	20.65926214445987	27.289758484813003	31.385982226279204	20.664997144447923
13	30.59801712375413	21.43993901783346	26.05637504988255	21.905668808529857
14	20.451607353225818	22.36829214089939	28.104009003949983	29.07609150192481
15	22.59100130710208	30.116038166424918	24.910928281435567	22.382032245037433
16	21.95477474592755	28.749077023439423	26.164264737157783	23.131883493475243
17	21.654882038218997	27.542934838452222	27.416764838715075	23.385418284613714
18	22.391590578350854	26.780299319207714	29.260806293206652	21.567303809234783
19	22.610595890394592	26.054104945720614	30.04685972906904	21.28843943481575
20	23.157690993421475	25.942391925120017	29.52772275098391	21.372194330474596
21	22.337347036797194	26.444323903240992	29.781018583789542	21.437310476172268
22	21.718086517253987	26.687583486067535	29.65317587572255	21.94115412095593
23	22.263508911951025	26.715063694343616	29.890222541895366	21.13120485180999
24	22.049760683229668	26.794995256677094	28.637244523672408	22.517999536420835
25	22.16792557881682	26.787348590026355	29.356389626340857	21.68833620481597
26	21.607448809151148	27.4970548385478	29.401911188746016	21.493585163555032
27	21.177682247546496	27.40756494290091	29.220302855791036	22.194449953761563
28	21.64568214240483	26.997751402088017	29.453048271972815	21.903518183534338
29	21.59956318416758	27.112451401849057	28.97716275213091	22.310822661852452
30	21.845690266988147	27.248060255733208	29.82845181285739	21.077797664421254
31	22.165058078822796	27.10169827687146	29.50358795936753	21.229655684938216
32	21.511507038517692	27.063703901950614	29.242525980744738	22.182263078786953
33	21.570410267561645	26.724860985989874	29.688063792316534	22.016664954131947
34	21.06835881027425	26.96835952714925	30.11735243725552	21.84592922532098
35	20.923550060575938	28.183940566283454	29.42090837620644	21.471600996934164
36	22.292542349390537	26.72020129849958	29.200349834999273	21.78690651711061
37	21.416640580381998	26.91901463141872	29.447313271984765	22.217031516214515
38	21.277566830671734	26.099745987292195	30.377697540879794	22.24498964115627
39	20.72617047765381	26.091860362308623	31.029336914522215	22.15263224551535
40	20.276331416090976	26.170597132977925	31.049528893646816	22.503542557284288
41	20.83824193575366	25.48622046773704	31.13901878929371	22.536518807215586
42	20.001051416664474	26.679817340250377	30.69909649854355	22.620034744541595
43	20.12769933306729	26.283743903575534	30.419754207458844	23.168802555898328
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	262.0
1	233.5
2	205.0
3	478.0
4	751.0
5	751.0
6	1045.5
7	1340.0
8	1422.5
9	1505.0
10	2192.0
11	2879.0
12	2879.0
13	5138.0
14	7397.0
15	12095.5
16	16794.0
17	15824.5
18	14855.0
19	14855.0
20	15662.0
21	16469.0
22	14624.0
23	12779.0
24	14692.5
25	16606.0
26	16606.0
27	19104.5
28	21603.0
29	23797.5
30	25992.0
31	28651.0
32	31310.0
33	31310.0
34	34651.0
35	37992.0
36	40273.0
37	42554.0
38	44603.0
39	46652.0
40	46652.0
41	47880.0
42	49108.0
43	50601.5
44	52095.0
45	53678.0
46	55261.0
47	55261.0
48	64152.0
49	73043.0
50	68504.5
51	63966.0
52	59710.0
53	55454.0
54	55454.0
55	51109.5
56	46765.0
57	41773.0
58	36781.0
59	33359.0
60	29937.0
61	29937.0
62	26327.0
63	22717.0
64	19788.0
65	16859.0
66	14515.0
67	12171.0
68	12171.0
69	10516.0
70	8861.0
71	7653.0
72	6445.0
73	5263.5
74	4082.0
75	4082.0
76	3310.5
77	2539.0
78	1928.5
79	1318.0
80	1068.5
81	819.0
82	819.0
83	598.5
84	378.0
85	290.5
86	203.0
87	160.5
88	118.0
89	118.0
90	95.5
91	73.0
92	43.5
93	14.0
94	11.0
95	8.0
96	8.0
97	6.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	836966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.29242676715069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.99330312338273	57.66997176539391
2	7.809577265252483	10.354316578783104
3	1.995460221073628	3.968517017168575
4	0.9109402171207709	2.415537505309242
5	0.5126701046939899	1.69930726855669
6	0.348489846436327	1.386134257438548
7	0.2402818553269035	1.1150207108413694
8	0.17858604300097558	0.9471121741821918
9	0.12337560012237463	0.7360981142369208
>10	0.8049710644975281	9.688217006619098
>50	0.05435586935214674	2.4864223487506627
>100	0.024738478687526315	3.2155456760506738
>500	0.0014445826900790868	0.6225915053577881
>1k	0.0012640098538192009	1.1944276178542605
>5k	5.417185087796576E-4	2.500780453456941
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8596	1.027042914526994	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6160	0.7359916651333507	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6135	0.7330046859729069	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2618	0.3127964576816741	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1422	0.16989937464604296	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1275	0.15233593718263347	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1222	0.1460035413624926	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1211	0.14468927053189737	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1120	0.13381666638788195	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1110	0.13262187472370443	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	881	0.10526114561403928	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.1947916641775174E-4	0.0
11	0.0	0.0	0.0	1.1947916641775174E-4	0.0
12	0.0	0.0	0.0	5.973958320887587E-4	0.0
13	0.0	0.0	0.0	7.168749985065104E-4	0.0
14	0.0	0.0	0.0	7.168749985065104E-4	0.0
15	0.0	0.0	0.0	7.168749985065104E-4	0.0
16	0.0	0.0	0.0	8.363541649242622E-4	0.0
17	0.0	0.0	0.0	0.0011947916641775174	0.0
18	0.0	0.0	0.0	0.0016727083298485243	0.0
19	0.0	0.0	0.0	0.0021506249955195312	0.0
20	0.0	0.0	0.0	0.005018124989545573	0.0
21	0.0	0.0	0.0	0.00955833331342014	0.0
22	0.0	0.0	0.0	0.014098541637294706	0.0
23	0.0	0.0	0.0	0.017085520797738497	0.0
24	0.0	0.0	0.0	0.02198416662086632	0.0
25	0.0	0.0	0.0	0.02544906244698112	0.0
26	0.0	0.0	0.0	0.029989270770855687	0.0
27	0.0	0.0	0.0	0.07240437484915756	0.0
28	0.0	0.0	0.0	0.1785018746281211	0.0
29	0.0	0.0	0.0	0.31578343684211785	0.0
30	0.0	0.0	0.0	0.5088617697732046	0.0
31	0.0	0.0	0.0	0.8214192691220432	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1915	0.0	22.798954	1
CGGGATA	45	0.0038249525	20.555555	24
TACCCCG	85	1.2444798E-6	19.588236	5
CTATTAT	110	3.8478902E-8	18.5	1
TAGGACC	100	2.8726026E-7	18.5	4
ACGTTCG	75	2.0664203E-4	17.266666	22
GTGTAGA	110	7.8013545E-7	16.818182	1
CGTGCAA	90	4.443634E-5	16.444445	11
TAACCGT	90	4.443634E-5	16.444445	7
ATCGGCT	70	0.0025917047	15.857142	3
AACCGTG	95	7.056013E-5	15.578948	8
GCTTCGT	95	7.056013E-5	15.578948	28
ATTATAC	155	7.197741E-9	15.5161295	3
CGTTCGC	120	1.9338713E-6	15.416667	23
TTACCCC	120	1.9338713E-6	15.416667	4
CAGTTCG	85	5.3619867E-4	15.235294	13
TGATCGC	75	0.004103166	14.8	9
TTAGGAC	150	8.090683E-8	14.8	3
CGAACTA	75	0.004103166	14.8	29
CTACTCG	75	0.004103166	14.8	14
>>END_MODULE