FastQCFastQC Report
Wed 25 May 2016
SRR2088554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1782791
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT327541.8372316216539124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT227261.274742804961434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT225801.2665533985756043No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95520.5357891082016905No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG51280.28763887634613367No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA42220.23681968329434017No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG42030.23575393862769106No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA41810.23451991848736056No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA37270.2090542301369033No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA35580.19957471178618244No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG34910.1958165595406304No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30040.16849984097967735No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC27330.1532989565237877No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT20690.11605398501563No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20640.11577352589282759No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA20060.11252020006831984No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19840.11128617992798931No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT18510.10382596726144566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA451.3236121E-424.6666665
TATACCG1301.8189894E-1221.3461535
GGTATCA81400.020.4318181
GTATTGG5800.019.1379321
CATTACG609.239535E-418.54
ATTGGAC6250.017.1683
ACCGTTA1107.813542E-716.8181828
GGACCCT8150.016.570556
TTAGACG803.3838736E-416.187515
CCGACCG1504.6748028E-916.0333339
TTGGACC8850.015.8870054
TTACGAT700.00259324515.8571436
TGGACCC8750.015.6457155
GACCGTT1455.3503754E-815.3103467
CGGTATA1152.2110838E-514.47826126
TAGACGT908.277169E-414.38888816
TTATGCG1551.2111741E-714.322584
TATACGC1051.6564388E-414.0952383
CCGTTTA1051.6564388E-414.09523827
ACACCCG1601.7848652E-713.8755
Produced by FastQC (version 0.11.5)