##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088554_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1782791
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.702400337448417	31.0	31.0	34.0	30.0	34.0
2	31.851198485969473	31.0	31.0	34.0	30.0	34.0
3	31.975351008615142	33.0	31.0	34.0	30.0	34.0
4	35.64528876351743	37.0	35.0	37.0	33.0	37.0
5	35.56216124043704	37.0	35.0	37.0	33.0	37.0
6	35.61792380598735	37.0	35.0	37.0	33.0	37.0
7	35.46312327132008	37.0	35.0	37.0	33.0	37.0
8	35.51175488321402	37.0	35.0	37.0	33.0	37.0
9	37.10141906706956	39.0	37.0	39.0	33.0	39.0
10	36.9666657505002	39.0	37.0	39.0	33.0	39.0
11	37.03232459665771	39.0	37.0	39.0	33.0	39.0
12	36.87597424487784	39.0	37.0	39.0	32.0	39.0
13	36.93048035355799	39.0	37.0	39.0	33.0	39.0
14	37.916118602797525	40.0	37.0	41.0	33.0	41.0
15	37.933746580502145	40.0	37.0	41.0	33.0	41.0
16	37.82376677916817	40.0	37.0	41.0	32.0	41.0
17	37.87566798351573	40.0	37.0	41.0	33.0	41.0
18	37.856920412992885	40.0	37.0	41.0	32.0	41.0
19	37.927291533331726	40.0	37.0	41.0	32.0	41.0
20	37.83495429357676	40.0	37.0	41.0	32.0	41.0
21	37.69719838163868	40.0	37.0	41.0	32.0	41.0
22	37.58354568763248	39.0	37.0	41.0	32.0	41.0
23	37.39518990167664	39.0	36.0	41.0	31.0	41.0
24	37.54297054450017	39.0	36.0	41.0	32.0	41.0
25	37.48135255338399	39.0	36.0	41.0	32.0	41.0
26	37.26156571353569	39.0	36.0	41.0	31.0	41.0
27	37.218480461254295	39.0	36.0	41.0	31.0	41.0
28	37.052100891243	39.0	36.0	41.0	31.0	41.0
29	36.82681817442426	39.0	35.0	41.0	30.0	41.0
30	36.66833969882056	39.0	35.0	40.0	30.0	41.0
31	36.383950782789455	38.0	35.0	40.0	30.0	41.0
32	36.21229465484176	38.0	35.0	40.0	30.0	41.0
33	36.02810144318656	38.0	35.0	40.0	30.0	41.0
34	35.82157975892856	38.0	35.0	40.0	29.0	41.0
35	35.61349703919304	38.0	35.0	40.0	27.0	41.0
36	35.52209204556227	38.0	35.0	40.0	27.0	41.0
37	35.34991145905493	38.0	34.0	40.0	26.0	41.0
38	35.198526355585145	38.0	34.0	40.0	25.0	41.0
39	35.04488243434031	38.0	34.0	40.0	24.0	41.0
40	34.83880443641459	38.0	34.0	40.0	24.0	41.0
41	34.68272444722909	38.0	33.0	40.0	23.0	41.0
42	34.36759104123815	38.0	33.0	40.0	21.0	41.0
43	33.96040309828802	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	1.0
13	3.0
14	4.0
15	6.0
16	17.0
17	48.0
18	123.0
19	275.0
20	700.0
21	1243.0
22	2444.0
23	4227.0
24	6705.0
25	10585.0
26	15540.0
27	21482.0
28	29391.0
29	38677.0
30	47303.0
31	58060.0
32	70232.0
33	86925.0
34	107494.0
35	132521.0
36	174894.0
37	256278.0
38	328246.0
39	389363.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.72508162762769	20.99578694305726	14.780924965405367	22.49820646390968
2	18.42459379702949	24.131095568689766	35.43331775850338	22.01099287577736
3	19.38494192532944	24.555093670542423	33.42825939776451	22.631705006363617
4	13.283441525114274	16.295235953064605	36.33634004210252	34.0849824797186
5	12.189146119763898	39.15332756335431	34.67361008665626	13.98391623022553
6	31.753918434634233	38.21479915480839	13.457101813953514	16.574180596603863
7	25.08684416737576	34.23026030533024	21.312593568174844	19.370301959119157
8	27.594934010773	34.40880058290624	18.86121255940825	19.135052846912508
9	24.665706748575687	14.549938831865317	21.968250905462277	38.81610351409672
10	16.493576644710455	28.438835511285394	32.509194852341075	22.558392991663073
11	32.96925999738612	23.127108000881762	23.229812131652	20.673819870080116
12	21.34164913329717	28.23174449500811	30.676282301178322	19.7503240705164
13	31.9397506494031	21.37995984947198	25.999233785676505	20.68105571544842
14	20.256777154472957	23.280743508352913	28.93541643411931	27.527062903054816
15	22.452996453313933	30.37125495921844	25.75949732750502	21.41625125996261
16	21.079139394354133	28.691753548228593	26.83573116534692	23.393375892070356
17	21.33906890936739	27.286428975690363	27.614061322948118	23.760440791994125
18	22.530571446681076	25.785299566802838	30.309273493079107	21.374855493436975
19	22.400382321876204	25.16408260979554	31.916753001333305	20.51878206699495
20	23.136138784636	25.005567113587627	31.17084391832806	20.687450183448313
21	21.784101445430228	25.862145366450694	31.89420408785999	20.45954910025909
22	20.70713841386904	26.36680351202132	31.31471944832569	21.611338625783954
23	21.59114556894218	26.19600390623466	31.9684135717535	20.244436953069652
24	21.363861495823123	26.50456503314185	30.21318819760701	21.91838527342801
25	21.386298225647312	26.448304933107696	31.262666235133562	20.902730606111426
26	20.821790103270658	27.59796296929926	31.13315021222342	20.447096715206662
27	20.032465948055606	27.399397910355166	30.89896684468342	21.669169296905807
28	20.513845986433633	27.17934968260441	30.900144772999187	21.40665955796277
29	20.58766282755522	27.08433013179896	30.2096544126597	22.11835262798612
30	20.71314023909701	27.35424399158398	31.870252878772664	20.06236289054634
31	21.44009028540081	27.049441016922344	31.517267026813574	19.99320167086327
32	20.767437125271552	27.04175643695756	30.675384831985355	21.515421605785534
33	20.46465345629409	26.796859530926508	31.41175830481531	21.326728707964087
34	19.736693757148203	27.12987669334207	32.09669557452332	21.03673397498641
35	19.367048633294647	28.905126848856654	30.994771681032717	20.733052836815983
36	21.367002637998507	26.927777849450663	30.832834583526612	20.87238492902421
37	20.485239155907784	27.5277920967741	30.795757887492144	21.191210859825972
38	20.19143017886	26.117587535499116	32.0291610177525	21.661821267888385
39	19.60639244869421	25.959689049361366	32.88972179015936	21.54419671178506
40	18.673585406253455	26.213785014620335	32.820560570476296	22.29206900864992
41	19.687725594306904	25.434052561405124	32.93846558570242	21.939756258585554
42	18.8221165576896	27.149060097341753	32.118571386101905	21.910251958866745
43	18.728891945270085	27.047870445834647	31.62529988091706	22.59793772797821
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	701.0
1	617.0
2	533.0
3	1314.0
4	2095.0
5	2095.0
6	3194.5
7	4294.0
8	4611.0
9	4928.0
10	7453.5
11	9979.0
12	9979.0
13	17926.5
14	25874.0
15	43933.5
16	61993.0
17	55448.0
18	48903.0
19	48903.0
20	48859.5
21	48816.0
22	38209.5
23	27603.0
24	30246.0
25	32889.0
26	32889.0
27	36068.0
28	39247.0
29	42015.0
30	44783.0
31	48601.0
32	52419.0
33	52419.0
34	58329.5
35	64240.0
36	70526.5
37	76813.0
38	81382.0
39	85951.0
40	85951.0
41	89553.0
42	93155.0
43	98870.0
44	104585.0
45	111486.5
46	118388.0
47	118388.0
48	151867.0
49	185346.0
50	171219.0
51	157092.0
52	141325.5
53	125559.0
54	125559.0
55	112537.5
56	99516.0
57	85859.0
58	72202.0
59	64454.5
60	56707.0
61	56707.0
62	49555.0
63	42403.0
64	37109.0
65	31815.0
66	27112.5
67	22410.0
68	22410.0
69	19047.0
70	15684.0
71	13179.5
72	10675.0
73	8616.5
74	6558.0
75	6558.0
76	5265.0
77	3972.0
78	3073.5
79	2175.0
80	1692.5
81	1210.0
82	1210.0
83	920.0
84	630.0
85	471.5
86	313.0
87	239.5
88	166.0
89	166.0
90	136.0
91	106.0
92	71.5
93	37.0
94	27.0
95	17.0
96	17.0
97	12.5
98	8.0
99	4.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1782791.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.88075962971204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.19925284545069	41.31479666141587
2	10.35491822780302	10.537322106683384
3	3.3273307598933632	5.078913498080439
4	1.4763434936330095	3.004699137217218
5	0.786076713317097	1.9998090150400636
6	0.550747834716766	1.6813480916884886
7	0.3625402612275331	1.2912426721327783
8	0.2680139781092666	1.0909403838064398
9	0.21684832406003285	0.9930066707343989
>10	1.2777209430512235	12.168582999940448
>50	0.10821388146938354	3.8197034716748757
>100	0.062482479777313166	5.952865928260369
>500	0.004533959966781449	1.6222101599200307
>1k	0.004423375577347755	4.226433913578894
>5k	2.2116877886738776E-4	0.8259874838758992
>10k+	3.3175316830108163E-4	4.392137805950456
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32754	1.8372316216539124	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22726	1.274742804961434	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22580	1.2665533985756043	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9552	0.5357891082016905	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5128	0.28763887634613367	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4222	0.23681968329434017	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4203	0.23575393862769106	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4181	0.23451991848736056	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3727	0.2090542301369033	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3558	0.19957471178618244	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3491	0.1958165595406304	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3004	0.16849984097967735	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2733	0.1532989565237877	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2069	0.11605398501563	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2064	0.11577352589282759	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2006	0.11252020006831984	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1984	0.11128617992798931	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1851	0.10382596726144566	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.6827547368143545E-4	0.0
10	0.0	0.0	0.0	1.6827547368143545E-4	0.0
11	0.0	0.0	0.0	3.926427719233494E-4	0.0
12	0.0	0.0	0.0	6.170100701652633E-4	0.0
13	0.0	0.0	0.0	8.413773684071773E-4	0.0
14	5.6091824560478485E-5	0.0	0.0	0.0011218364912095697	0.0
15	5.6091824560478485E-5	0.0	0.0	0.0014022956140119622	0.0
16	5.6091824560478485E-5	0.0	0.0	0.002411948456100575	0.0
17	5.6091824560478485E-5	0.0	0.0	0.003814244070112537	0.0
18	5.6091824560478485E-5	0.0	0.0	0.004767805087640672	0.0
19	5.6091824560478485E-5	0.0	0.0	0.006282284350773591	0.0
20	5.6091824560478485E-5	0.0	0.0	0.009759977473523257	0.0
21	5.6091824560478485E-5	0.0	0.0	0.01626662912253876	0.0
22	5.6091824560478485E-5	0.0	0.0	0.026755800315348237	0.0
23	5.6091824560478485E-5	0.0	0.0	0.032084523648593696	0.0
24	5.6091824560478485E-5	0.0	0.0	0.04234932754316126	0.0
25	5.6091824560478485E-5	0.0	0.0	0.04846333642025341	0.0
26	5.6091824560478485E-5	0.0	0.0	0.05878423213938145	0.0
27	5.6091824560478485E-5	0.0	0.0	0.11515651582266233	0.0
28	5.6091824560478485E-5	0.0	0.0	0.25779802567995913	0.0
29	5.6091824560478485E-5	0.0	0.0	0.4807630283078611	0.0
30	1.1218364912095697E-4	0.0	0.0	0.7811908406537839	0.0
31	1.1218364912095697E-4	0.0	0.0	1.2003650455942396	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGA	45	1.3236121E-4	24.666666	5
TATACCG	130	1.8189894E-12	21.346153	5
GGTATCA	8140	0.0	20.431818	1
GTATTGG	580	0.0	19.137932	1
CATTACG	60	9.239535E-4	18.5	4
ATTGGAC	625	0.0	17.168	3
ACCGTTA	110	7.813542E-7	16.818182	8
GGACCCT	815	0.0	16.57055	6
TTAGACG	80	3.3838736E-4	16.1875	15
CCGACCG	150	4.6748028E-9	16.033333	9
TTGGACC	885	0.0	15.887005	4
TTACGAT	70	0.002593245	15.857143	6
TGGACCC	875	0.0	15.645715	5
GACCGTT	145	5.3503754E-8	15.310346	7
CGGTATA	115	2.2110838E-5	14.478261	26
TAGACGT	90	8.277169E-4	14.388888	16
TTATGCG	155	1.2111741E-7	14.32258	4
TATACGC	105	1.6564388E-4	14.095238	3
CCGTTTA	105	1.6564388E-4	14.095238	27
ACACCCG	160	1.7848652E-7	13.875	5
>>END_MODULE