FastQCFastQC Report
Wed 25 May 2016
SRR2088553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204450
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT447271.3957777465711745No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT316550.9878450280079264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT316430.9874705487681193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138740.4329604144236921No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG68070.21242334878060196No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA55410.17291578898094837No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA55350.1727285493610448No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG55270.1724788965345067No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG49270.15375493454414954No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA46270.14439295354897097No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA44850.13996161587791978No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT40400.12607467740173822No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34520.10772519465118818No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.10338747679008878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107250.020.130071
ATATTCG650.001580767917.0769238
ATTGGAC9800.016.801023
GTATTGG10400.016.7211531
TTGGACC12550.015.9203194
TAGGTCG2100.015.85714221
GGACCCT12800.015.4648446
GGTAAAC5000.015.1735
TGGACCC13350.015.1048695
GACCCTC13300.014.6052637
TCTAATA8000.014.5687492
CGCGATA908.279709E-414.38888814
CTAATAC9050.013.9005533
TAATACT8800.013.8754
CGAACTA3350.013.8059724
ATTAGAC2551.8189894E-1213.7843133
TCTAGAC2151.9826984E-1013.7674423
CTAGTAC4300.013.7674423
GTATTAG7350.013.5918371
ACGTTTA1951.0279109E-813.28205226
Produced by FastQC (version 0.11.5)