##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088553_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3204450
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.733892555664777	31.0	31.0	34.0	30.0	34.0
2	31.888758757353056	31.0	31.0	34.0	30.0	34.0
3	32.018592582190394	33.0	31.0	34.0	30.0	34.0
4	35.6882088970026	37.0	35.0	37.0	35.0	37.0
5	35.59431259654543	37.0	35.0	37.0	33.0	37.0
6	35.635852954485166	37.0	35.0	37.0	33.0	37.0
7	35.49579709466523	37.0	35.0	37.0	33.0	37.0
8	35.53001357487244	37.0	35.0	37.0	33.0	37.0
9	37.142996458050526	39.0	37.0	39.0	33.0	39.0
10	36.99796439326561	39.0	37.0	39.0	33.0	39.0
11	37.05726942220974	39.0	37.0	39.0	33.0	39.0
12	36.913112390581844	39.0	37.0	39.0	33.0	39.0
13	36.965008347766386	39.0	37.0	39.0	33.0	39.0
14	38.01342851347345	40.0	37.0	41.0	33.0	41.0
15	38.021731654418076	40.0	37.0	41.0	33.0	41.0
16	37.91820187551686	40.0	37.0	41.0	33.0	41.0
17	37.9318382249684	40.0	37.0	41.0	33.0	41.0
18	37.897742202249994	40.0	37.0	41.0	33.0	41.0
19	37.95813790198006	40.0	37.0	41.0	33.0	41.0
20	37.86098862519309	40.0	37.0	41.0	32.0	41.0
21	37.71693207882788	40.0	37.0	41.0	32.0	41.0
22	37.602696874658676	39.0	37.0	41.0	32.0	41.0
23	37.43284120519902	39.0	36.0	41.0	32.0	41.0
24	37.58496528265381	39.0	37.0	41.0	32.0	41.0
25	37.53117758117617	39.0	36.0	41.0	32.0	41.0
26	37.33049914962006	39.0	36.0	41.0	31.0	41.0
27	37.28853282154504	39.0	36.0	41.0	31.0	41.0
28	37.14311129835073	39.0	36.0	41.0	31.0	41.0
29	36.93498072992245	39.0	36.0	41.0	30.0	41.0
30	36.81562202562062	39.0	35.0	41.0	30.0	41.0
31	36.58412644915664	39.0	35.0	40.0	30.0	41.0
32	36.424909110767835	39.0	35.0	40.0	30.0	41.0
33	36.26572297898235	38.0	35.0	40.0	30.0	41.0
34	36.080114528234176	38.0	35.0	40.0	29.0	41.0
35	35.89329588540935	38.0	35.0	40.0	28.0	41.0
36	35.832069153832954	38.0	35.0	40.0	28.0	41.0
37	35.69975783675826	38.0	35.0	40.0	27.0	41.0
38	35.569903415562734	38.0	35.0	40.0	26.0	41.0
39	35.453734026119925	38.0	35.0	40.0	26.0	41.0
40	35.2917140226872	38.0	34.0	40.0	25.0	41.0
41	35.17763360327045	38.0	34.0	40.0	24.0	41.0
42	34.91795659161478	38.0	34.0	40.0	23.0	41.0
43	34.53862035606734	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	5.0
13	4.0
14	6.0
15	13.0
16	32.0
17	88.0
18	218.0
19	475.0
20	1041.0
21	2088.0
22	4017.0
23	6978.0
24	11061.0
25	17074.0
26	25216.0
27	35731.0
28	48716.0
29	63732.0
30	81243.0
31	99870.0
32	122622.0
33	150883.0
34	187224.0
35	233290.0
36	305621.0
37	442089.0
38	605687.0
39	759425.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5597996536067	20.9541731030286	14.19806831125466	23.287958932110033
2	18.294184649471827	23.50219226388304	35.63460188175818	22.569021204886955
3	19.17383638377881	24.586528109347938	32.8203903946075	23.419245112265756
4	13.446987782614803	16.496590678587587	36.54570987220896	33.51071166658865
5	12.608559971289926	38.74948899187068	34.84470033859164	13.797250698247748
6	31.953470954453962	38.12950740376664	14.178564184181372	15.73845745759803
7	25.80767370375571	33.48137745947042	21.7666058137902	18.944343022983663
8	26.99826803351589	34.84685359422054	19.239245424331788	18.915632947931783
9	24.92318494593456	14.624724991808266	21.569598527048324	38.88249153520885
10	16.27936151289613	28.225967014620295	33.168624881024826	22.326046591458752
11	33.5616096365991	22.922623227074848	23.180202530855528	20.33556460547052
12	21.16366303109738	27.595812073834825	30.93457535614536	20.305949538922434
13	31.252820296774797	21.253413222237825	25.981026385183103	21.512740095804272
14	20.53569255254412	22.62572360311442	28.335190126230707	28.503393718110754
15	22.85864969027446	30.00486823011749	25.16434957637036	21.972132503237685
16	21.81616189985801	28.36299520978639	26.40846323081964	23.412379659535958
17	21.664092121892992	27.333083680506796	27.419588384902244	23.583235812697968
18	22.56337280968653	26.106414517311865	29.611290549080188	21.718922123921423
19	22.77807424050929	25.398586340869727	30.76409368222316	21.059245736397823
20	23.316544180748647	25.358017756557288	30.107163475791477	21.218274586902588
21	22.275055001638346	26.01535364883209	30.59039772815928	21.119193621370282
22	21.445895551498698	26.433054034233646	30.15578336375977	21.965267050507887
23	22.033110206119616	26.35129273354242	30.744589555149872	20.871007505188096
24	21.930222034982602	26.437859851144502	29.272574076674623	22.35934403719827
25	21.995662282138902	26.37607077657632	30.12545054533539	21.502816395949385
26	21.44870414579725	27.26068436081075	30.1520385713617	21.138572922030303
27	20.86632651469051	27.184290595890094	29.83463620902183	22.114746680397573
28	21.13208194854031	27.01096912106602	29.954063879916994	21.90288505047668
29	21.433943422428186	26.918535162040286	29.42841985364103	22.219101561890493
30	21.41440808875158	27.086177035060622	30.59361200830096	20.905802867886845
31	21.85538860022781	26.873535240056796	30.405935495950942	20.865140663764453
32	21.248763438343556	26.91107678384746	29.740048994367207	22.100110783441774
33	21.14830938226529	26.590148075332742	30.426563060743657	21.83497948165832
34	20.738192201469833	26.71497448860179	30.93432570331882	21.612507606609558
35	20.49659067858759	28.068186428248215	30.03105057030068	21.40417232286352
36	21.94061383388725	26.496746711604175	29.980277426703488	21.582362027805083
37	21.015774937976875	26.880806378629718	30.10550952581566	21.997909157577745
38	20.7847836602225	25.815381734775077	31.20011234377194	22.199722261230477
39	20.334347547941146	25.66949086426688	31.867933654761348	22.12822793303063
40	19.592941066329637	25.955124904429777	31.929847555742796	22.522086473497794
41	20.221192404312752	25.180046497838944	32.07498946777138	22.523771630076926
42	19.354397790572484	26.655900388522213	31.49985176863424	22.48985005227106
43	19.462466257860164	26.30473248139306	31.16949866591771	23.063302594829064
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1223.0
1	1117.5
2	1012.0
3	2324.5
4	3637.0
5	3637.0
6	5272.5
7	6908.0
8	7328.0
9	7748.0
10	11450.5
11	15153.0
12	15153.0
13	26490.0
14	37827.0
15	61850.0
16	85873.0
17	78838.0
18	71803.0
19	71803.0
20	72265.5
21	72728.0
22	58732.5
23	44737.0
24	50536.0
25	56335.0
26	56335.0
27	63864.5
28	71394.0
29	77574.0
30	83754.0
31	92461.5
32	101169.0
33	101169.0
34	113263.5
35	125358.0
36	136232.0
37	147106.0
38	156379.0
39	165652.0
40	165652.0
41	172911.0
42	180170.0
43	189643.0
44	199116.0
45	207723.0
46	216330.0
47	216330.0
48	260342.5
49	304355.0
50	286082.0
51	267809.0
52	245371.5
53	222934.0
54	222934.0
55	201933.5
56	180933.0
57	159437.0
58	137941.0
59	125154.0
60	112367.0
61	112367.0
62	99549.0
63	86731.0
64	75106.0
65	63481.0
66	54231.5
67	44982.0
68	44982.0
69	38416.0
70	31850.0
71	27333.5
72	22817.0
73	18498.5
74	14180.0
75	14180.0
76	11382.0
77	8584.0
78	6667.0
79	4750.0
80	3768.5
81	2787.0
82	2787.0
83	2124.5
84	1462.0
85	1092.0
86	722.0
87	532.5
88	343.0
89	343.0
90	296.5
91	250.0
92	167.0
93	84.0
94	59.0
95	34.0
96	34.0
97	24.5
98	15.0
99	10.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3204450.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.50036079754973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7242249275713	36.91928726769545
2	11.96419093273927	11.366067719117765
3	4.006752059639306	5.709665053775775
4	1.872187938241994	3.5571841018926196
5	1.016957940178658	2.4152934537209623
6	0.6575413040358689	1.8740069508597066
7	0.42771723143717777	1.4221705968816518
8	0.3315395902215618	1.2598600123356782
9	0.2412850751302951	1.031501531137762
>10	1.528493683378155	13.648143604273594
>50	0.14326839936482122	4.687648143137834
>100	0.07674903489894315	6.825267148888026
>500	0.005336540117441904	1.7118235407273121
>1k	0.003228277354779002	3.024670146732312
>5k	2.635328452880818E-4	0.732610403772371
>10k+	2.635328452880818E-4	3.8148003250511855
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	44727	1.3957777465711745	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31655	0.9878450280079264	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31643	0.9874705487681193	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13874	0.4329604144236921	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6807	0.21242334878060196	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5541	0.17291578898094837	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5535	0.1727285493610448	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5527	0.1724788965345067	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4927	0.15375493454414954	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4627	0.14439295354897097	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4485	0.13996161587791978	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4040	0.12607467740173822	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3452	0.10772519465118818	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3313	0.10338747679008878	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.241320663452386E-5	0.0
10	3.120660331726193E-5	0.0	0.0	1.872396199035716E-4	0.0
11	3.120660331726193E-5	0.0	0.0	3.744792398071432E-4	0.0
12	3.120660331726193E-5	0.0	0.0	6.553386696625006E-4	0.0
13	3.120660331726193E-5	0.0	0.0	7.177518762970244E-4	0.0
14	3.120660331726193E-5	0.0	0.0	7.801650829315483E-4	0.0
15	3.120660331726193E-5	0.0	0.0	0.0011858509260559535	0.0
16	6.241320663452386E-5	0.0	0.0	0.0016539499758148825	0.0
17	9.36198099517858E-5	0.0	0.0	0.002215668835525597	0.0
18	9.36198099517858E-5	0.0	0.0	0.0025901480753327405	0.0
19	1.2482641326904773E-4	0.0	0.0	0.003339106554947027	0.0
20	1.2482641326904773E-4	0.0	0.0	0.005866841423645243	0.0
21	1.5603301658630967E-4	0.0	0.0	0.010610245127869056	0.0
22	1.5603301658630967E-4	0.0	0.0	0.01697639220459049	0.0
23	1.5603301658630967E-4	0.0	0.0	0.020533944982758352	0.0
24	1.5603301658630967E-4	0.0	0.0	0.026244753389817285	0.0
25	1.5603301658630967E-4	0.0	0.0	0.02998954578788872	0.0
26	1.5603301658630967E-4	0.0	0.0	0.03891463433662563	0.0
27	1.5603301658630967E-4	0.0	0.0	0.0883771005944858	0.0
28	1.5603301658630967E-4	0.0	0.0	0.22696562592644604	0.0
29	1.5603301658630967E-4	0.0	0.0	0.42369205323846526	0.0
30	1.872396199035716E-4	0.0	0.0	0.6840175381110642	0.0
31	1.872396199035716E-4	0.0	0.0	1.1113919705409665	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10725	0.0	20.13007	1
ATATTCG	65	0.0015807679	17.076923	8
ATTGGAC	980	0.0	16.80102	3
GTATTGG	1040	0.0	16.721153	1
TTGGACC	1255	0.0	15.920319	4
TAGGTCG	210	0.0	15.857142	21
GGACCCT	1280	0.0	15.464844	6
GGTAAAC	500	0.0	15.17	35
TGGACCC	1335	0.0	15.104869	5
GACCCTC	1330	0.0	14.605263	7
TCTAATA	800	0.0	14.568749	2
CGCGATA	90	8.279709E-4	14.388888	14
CTAATAC	905	0.0	13.900553	3
TAATACT	880	0.0	13.875	4
CGAACTA	335	0.0	13.80597	24
ATTAGAC	255	1.8189894E-12	13.784313	3
TCTAGAC	215	1.9826984E-10	13.767442	3
CTAGTAC	430	0.0	13.767442	3
GTATTAG	735	0.0	13.591837	1
ACGTTTA	195	1.0279109E-8	13.282052	26
>>END_MODULE