FastQCFastQC Report
Wed 25 May 2016
SRR2088552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4077730
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT868802.1305971704845588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT613611.5047832985509095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT605181.4841100318069123No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246570.604674659675849No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG152500.373982583447163No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA140300.34406397677138995No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG124020.30413980327289936No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA123260.3022760212176873No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA107360.2632837387468028No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA106860.26205756634205796No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG106510.2611992456587366No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC78530.19258263788921776No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT77090.18905126136355277No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA65600.16087381950251733No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT64290.15766124780208596No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA58630.1437809761803749No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT55270.13554109762048985No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52300.12825763353630573No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA49400.1211458335887859No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG48720.119478239118333No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG48170.11812944947311371No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA47360.11614305017742714No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT45460.11148359503939692No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT44680.10957076608799504No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44640.10947267229561544No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA44210.10841816402753493No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC43090.10567153784090658No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42820.1050094047423444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG16000.021.7375011
GGTATCA207700.021.5907551
ATTGGAC16800.020.3720233
TTGGACC22150.020.0451474
GGACCCT22250.019.3730346
TGGACCC22650.019.275945
TATTGGA19450.017.406172
GACCCTC24750.017.0424257
ACCCTCG26900.015.749078
ACTCTGT21200.015.53301937
GTATTAG6200.015.2177411
CCCTCGC27300.014.9761919
TAAACGT2350.014.9574464
TTAACGG3400.014.69117535
ACCGTTA1904.5656634E-1014.6052648
CCTCGCA28500.014.345613510
CGTTTAG2500.014.06000126
TATACAC23950.013.90396637
TATAGCG800.006302249613.8750015
AATACCG13050.013.7509585
Produced by FastQC (version 0.11.5)