##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088552_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4077730
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.687612470663826	31.0	31.0	34.0	30.0	34.0
2	31.828564667106455	31.0	31.0	34.0	30.0	34.0
3	31.937408558192917	31.0	31.0	34.0	30.0	34.0
4	35.61536443070042	37.0	35.0	37.0	33.0	37.0
5	35.53979223734774	37.0	35.0	37.0	33.0	37.0
6	35.60977038695549	37.0	35.0	37.0	33.0	37.0
7	35.450851331500616	37.0	35.0	37.0	33.0	37.0
8	35.513573483286045	37.0	35.0	37.0	33.0	37.0
9	37.089527016256596	39.0	37.0	39.0	33.0	39.0
10	36.961193360031196	39.0	37.0	39.0	33.0	39.0
11	37.020291927126124	39.0	37.0	39.0	33.0	39.0
12	36.86428699300836	39.0	37.0	39.0	32.0	39.0
13	36.92696156930449	39.0	37.0	39.0	33.0	39.0
14	37.89065190682071	40.0	37.0	41.0	33.0	41.0
15	37.89957623481692	40.0	37.0	41.0	33.0	41.0
16	37.79141311464957	40.0	37.0	41.0	32.0	41.0
17	37.86830172669598	40.0	37.0	41.0	33.0	41.0
18	37.849169022961306	40.0	37.0	41.0	32.0	41.0
19	37.932427600650364	40.0	37.0	41.0	32.0	41.0
20	37.83913795175257	40.0	37.0	41.0	32.0	41.0
21	37.710812878734004	40.0	37.0	41.0	32.0	41.0
22	37.604230294796366	40.0	37.0	41.0	32.0	41.0
23	37.41731747810669	39.0	36.0	41.0	32.0	41.0
24	37.550643617895254	39.0	36.0	41.0	32.0	41.0
25	37.48101198460908	39.0	36.0	41.0	32.0	41.0
26	37.256944427414275	39.0	36.0	41.0	31.0	41.0
27	37.21756957915311	39.0	36.0	41.0	31.0	41.0
28	37.04888111768067	39.0	35.0	41.0	31.0	41.0
29	36.81741434572667	39.0	35.0	41.0	30.0	41.0
30	36.640092894821386	39.0	35.0	40.0	30.0	41.0
31	36.33110848437734	38.0	35.0	40.0	30.0	41.0
32	36.16904012771812	38.0	35.0	40.0	30.0	41.0
33	35.98042759084098	38.0	35.0	40.0	30.0	41.0
34	35.7761369683623	38.0	35.0	40.0	29.0	41.0
35	35.54753576131819	38.0	35.0	40.0	27.0	41.0
36	35.441037292807515	38.0	35.0	40.0	26.0	41.0
37	35.27016575398567	38.0	34.0	40.0	25.0	41.0
38	35.105686006675285	38.0	34.0	40.0	25.0	41.0
39	34.9466203010008	38.0	34.0	40.0	24.0	41.0
40	34.71122683453785	38.0	34.0	40.0	23.0	41.0
41	34.527936621600745	38.0	33.0	40.0	22.0	41.0
42	34.18896984351588	38.0	33.0	40.0	20.0	41.0
43	33.769301302440326	38.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	5.0
14	10.0
15	11.0
16	47.0
17	117.0
18	308.0
19	652.0
20	1452.0
21	2940.0
22	5434.0
23	9697.0
24	16152.0
25	24491.0
26	36087.0
27	49981.0
28	68181.0
29	88769.0
30	110732.0
31	134597.0
32	162266.0
33	200399.0
34	246983.0
35	305373.0
36	403450.0
37	596167.0
38	747597.0
39	865826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.558072751261115	21.285715336719203	15.346920958474444	21.809290953545233
2	18.423853467492943	24.453948642994998	35.71455196886503	21.407645920647024
3	19.42948650352034	24.376282882878463	34.26813447677997	21.926096136821222
4	13.04723461337558	16.25036478629041	36.09542564122686	34.60697495910715
5	11.860029967653572	39.4594786805404	34.6076861391019	14.072805212704125
6	31.582449058667443	38.23401745579035	13.038504265853796	17.145029219688404
7	24.649940040169408	34.83933462980629	20.83735313520022	19.673372194824083
8	27.880806232879568	34.178550320889315	18.479080272602648	19.461563173628466
9	24.24748573348407	14.523546188688313	22.354618868831434	38.874349208996186
10	16.519190824306662	28.700061063385757	32.223957937381826	22.556790174925755
11	32.80933264340699	22.975748762178956	23.465163215808797	20.749755378605254
12	21.74491690229613	28.53492016391473	30.47644154958764	19.243721384201503
13	32.76580352303856	21.276690707820283	26.140990207787173	19.816515561353988
14	20.088897499343997	23.55565473927896	29.24050881250107	27.114938948875967
15	22.02441064023366	30.679397606021	26.104621934262447	21.191569819482897
16	20.70301368653638	28.759971847081587	27.163274665071008	23.373739801311025
17	21.235932737086564	27.17995060977554	27.569162254489633	24.014954398648268
18	22.78341135877069	25.36337619214612	30.91124228431014	20.941970164773046
19	22.249094471679097	24.647021749846115	32.80040610830045	20.30347767017434
20	23.176816513109006	24.347124503093635	32.06202960961123	20.41402937418613
21	21.75703148565501	25.324923425533324	32.99529395031059	19.922751138501077
22	20.21232401360561	25.937887010665246	32.29821493821317	21.551574037515973
23	21.53399072523193	25.910371701902772	32.972977612544234	19.58265996032106
24	21.06770188315558	26.08318844062751	30.919114311148604	21.92999536506831
25	21.088375149899576	26.21142155071572	32.15862747165703	20.54157582772768
26	20.439558283652914	27.529605932712563	32.06806237784258	19.962773405791946
27	19.29929151758454	27.29319989307777	31.814857776262773	21.592650813074922
28	20.067709240190005	27.06412636442334	31.59395055582395	21.274213839562698
29	20.315126308019412	26.750030041223916	30.711327135440552	22.22351651531612
30	20.336412660965784	27.207784723363243	32.883074651828345	19.572727963842627
31	21.378585634654577	26.977337881615505	32.37914231692633	19.26493416680359
32	20.595723601121215	26.86426026244994	31.21567636896018	21.324339767468665
33	20.128135016295833	26.705985928445486	32.07512513089391	21.09075392436478
34	19.311945616801506	27.332658121062455	32.86676655884524	20.4886297032908
35	18.71224921708892	29.64521927641114	31.477660364957956	20.164871141541983
36	21.350040341072116	27.143582336250805	31.256066487972472	20.250310834704603
37	20.26080687048922	28.148626809523925	31.111010292491166	20.47955602749569
38	19.896437478695255	26.340071559421542	32.68477805053302	21.078712911350188
39	19.368325023971668	26.0721528889848	33.52630017190938	21.033221915134156
40	18.041116013075904	26.482945168022404	33.42403739335366	22.05190142554804
41	19.496680751300353	25.47571810786884	33.57794655359722	21.44965458723358
42	18.397221002861887	27.649917969066124	32.63222430126566	21.320636726806335
43	18.453158987966344	27.522690320349803	31.95608831384128	22.068062377842573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1296.0
1	1298.0
2	1300.0
3	3052.0
4	4804.0
5	4804.0
6	7411.5
7	10019.0
8	11018.5
9	12018.0
10	18275.5
11	24533.0
12	24533.0
13	44117.0
14	63701.0
15	111172.0
16	158643.0
17	142643.5
18	126644.0
19	126644.0
20	126441.0
21	126238.0
22	96804.0
23	67370.0
24	73680.5
25	79991.0
26	79991.0
27	86987.5
28	93984.0
29	98234.0
30	102484.0
31	110010.5
32	117537.0
33	117537.0
34	128291.5
35	139046.0
36	151983.0
37	164920.0
38	173696.0
39	182472.0
40	182472.0
41	189538.0
42	196604.0
43	209007.5
44	221411.0
45	241462.0
46	261513.0
47	261513.0
48	358460.5
49	455408.0
50	418252.5
51	381097.0
52	339061.0
53	297025.0
54	297025.0
55	260893.0
56	224761.0
57	189184.0
58	153607.0
59	136483.5
60	119360.0
61	119360.0
62	104314.5
63	89269.0
64	77652.5
65	66036.0
66	56165.0
67	46294.0
68	46294.0
69	39613.0
70	32932.0
71	27668.0
72	22404.0
73	18258.0
74	14112.0
75	14112.0
76	11325.5
77	8539.0
78	6635.5
79	4732.0
80	3718.0
81	2704.0
82	2704.0
83	2077.0
84	1450.0
85	1113.0
86	776.0
87	578.5
88	381.0
89	381.0
90	284.0
91	187.0
92	130.0
93	73.0
94	55.5
95	38.0
96	38.0
97	26.0
98	14.0
99	8.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4077730.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.76814992648157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.78801425301691	30.897371276973157
2	12.59275072896343	10.267662994103825
3	4.597801899538596	5.623316315179537
4	2.171700263532984	3.5414480775636887
5	1.1972825222313652	2.4405496685342145
6	0.7203472345524741	1.7620334434416987
7	0.4629209194421037	1.3210700613544266
8	0.3491861403199947	1.1388538336651977
9	0.25026224682623377	0.918245591959503
>10	1.587226183546084	12.309064525037462
>50	0.1576451154472412	4.482591632490086
>100	0.10812707428544813	8.264576551074123
>500	0.009812493467666685	2.652369378114757
>1k	0.005839336595182053	5.431106330954145
>5k	4.2139542439458114E-4	1.1085938990385305
>10k+	6.621928097629132E-4	7.841146420515671
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	86880	2.1305971704845588	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	61361	1.5047832985509095	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	60518	1.4841100318069123	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24657	0.604674659675849	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	15250	0.373982583447163	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	14030	0.34406397677138995	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	12402	0.30413980327289936	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	12326	0.3022760212176873	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10736	0.2632837387468028	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	10686	0.26205756634205796	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10651	0.2611992456587366	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	7853	0.19258263788921776	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7709	0.18905126136355277	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6560	0.16087381950251733	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	6429	0.15766124780208596	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5863	0.1437809761803749	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	5527	0.13554109762048985	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5230	0.12825763353630573	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4940	0.1211458335887859	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	4872	0.119478239118333	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	4817	0.11812944947311371	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	4736	0.11614305017742714	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	4546	0.11148359503939692	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	4468	0.10957076608799504	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4464	0.10947267229561544	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4421	0.10841816402753493	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4309	0.10567153784090658	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4282	0.1050094047423444	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	4.904689618979187E-5	0.0	0.0	0.0	0.0
7	4.904689618979187E-5	0.0	0.0	0.0	0.0
8	4.904689618979187E-5	0.0	0.0	0.0	0.0
9	9.809379237958374E-5	0.0	0.0	7.357034428468781E-5	0.0
10	1.2261724047447968E-4	0.0	0.0	9.809379237958374E-5	0.0
11	2.4523448094895936E-4	0.0	0.0	2.4523448094895936E-4	0.0
12	2.697579290438553E-4	0.0	0.0	5.395158580877105E-4	0.0
13	2.9428137713875124E-4	0.0	0.0	5.885627542775025E-4	0.0
14	4.1689861761323087E-4	0.0	0.0	7.357034428468781E-4	0.0
15	4.1689861761323087E-4	0.0	0.0	9.809379237958374E-4	0.0
16	4.6594551380302275E-4	0.0	0.0	0.001863782055212091	0.0
17	4.6594551380302275E-4	0.0	0.0	0.002525915153774281	0.0
18	4.6594551380302275E-4	0.0	0.0	0.0031144779080517835	0.0
19	4.6594551380302275E-4	0.0	0.0	0.0043651737608914765	0.0
20	4.6594551380302275E-4	0.0	0.0	0.007209893739899405	0.0
21	4.6594551380302275E-4	0.0	0.0	0.013536943348382556	0.0
22	4.6594551380302275E-4	0.0	0.0	0.021703251563982902	0.0
23	4.6594551380302275E-4	0.0	0.0	0.02636270670201313	0.0
24	4.6594551380302275E-4	0.0	0.0	0.0332047487204891	0.0
25	4.6594551380302275E-4	0.0	0.0	0.037300164552336715	0.0
26	4.6594551380302275E-4	0.0	0.0	0.04529480863127279	0.0
27	4.904689618979187E-4	0.0	0.0	0.09976138685003666	0.0
28	4.904689618979187E-4	0.0	0.0	0.23991289271236693	0.0
29	4.904689618979187E-4	0.0	0.0	0.4557192359474512	0.0
30	4.904689618979187E-4	0.0	0.0	0.7496818082609688	0.0
31	5.149924099928146E-4	0.0	0.0	1.1354111233455868	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	1600	0.0	21.737501	1
GGTATCA	20770	0.0	21.590755	1
ATTGGAC	1680	0.0	20.372023	3
TTGGACC	2215	0.0	20.045147	4
GGACCCT	2225	0.0	19.373034	6
TGGACCC	2265	0.0	19.27594	5
TATTGGA	1945	0.0	17.40617	2
GACCCTC	2475	0.0	17.042425	7
ACCCTCG	2690	0.0	15.74907	8
ACTCTGT	2120	0.0	15.533019	37
GTATTAG	620	0.0	15.217741	1
CCCTCGC	2730	0.0	14.976191	9
TAAACGT	235	0.0	14.957446	4
TTAACGG	340	0.0	14.691175	35
ACCGTTA	190	4.5656634E-10	14.605264	8
CCTCGCA	2850	0.0	14.3456135	10
CGTTTAG	250	0.0	14.060001	26
TATACAC	2395	0.0	13.903966	37
TATAGCG	80	0.0063022496	13.875001	5
AATACCG	1305	0.0	13.750958	5
>>END_MODULE