FastQCFastQC Report
Wed 25 May 2016
SRR2088551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3175654
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT585171.8426755559642203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT408451.286191757666295No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT405841.2779729781644977No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168390.5302529809607721No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG97520.3070863513468407No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA83980.26444946458272844No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG77810.24502039579878662No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA77540.2441701772296352No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA73310.23085008631292958No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG61410.19337749011699637No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA60430.19029151160674304No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52110.16409218384622506No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC48410.15244104049118704No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT42140.1326970759408928No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA38780.12211657819145286No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT38050.11981783909708048No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37680.11865272476157668No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA35990.11333098631022145No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG32660.10284495729068723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143900.021.7011811
GTATTGG9000.020.7611121
ATTGGAC9450.019.3809533
GGACCCT13300.018.2218046
TTGGACC13100.017.9351164
AACCGTC852.7255639E-517.4117647
ACGTTTA1951.8189894E-1216.12820626
TGGACCC14550.016.0206185
GCCGTCA2250.015.62222213
GACCCTC14450.015.4913497
TATACAC19300.015.33678837
CGCGAAT855.3678494E-415.23529435
TAGTACT5000.015.174
CCGTATA1601.0984877E-815.0312512
TCACGTT2109.094947E-1214.976191524
TATACTG3700.014.55
TATTGGA12200.014.4057372
CCCGTAT1551.2121927E-714.3225811
CTAATAC4700.014.1702143
CTAGGCA3950.014.0506324
Produced by FastQC (version 0.11.5)