##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088551_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3175654
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72383389374283	31.0	31.0	34.0	30.0	34.0
2	31.877020922304506	31.0	31.0	34.0	30.0	34.0
3	31.991579057416203	33.0	31.0	34.0	30.0	34.0
4	35.66539301825703	37.0	35.0	37.0	33.0	37.0
5	35.58320805730095	37.0	35.0	37.0	33.0	37.0
6	35.63851162626659	37.0	35.0	37.0	33.0	37.0
7	35.48164378109202	37.0	35.0	37.0	33.0	37.0
8	35.531710948358985	37.0	35.0	37.0	33.0	37.0
9	37.12588399114009	39.0	37.0	39.0	33.0	39.0
10	36.98882560883522	39.0	37.0	39.0	33.0	39.0
11	37.04924906806598	39.0	37.0	39.0	33.0	39.0
12	36.89848232836449	39.0	37.0	39.0	33.0	39.0
13	36.95539753386232	39.0	37.0	39.0	33.0	39.0
14	37.95550428352711	40.0	37.0	41.0	33.0	41.0
15	37.96517882615675	40.0	37.0	41.0	33.0	41.0
16	37.86044197510182	40.0	37.0	41.0	32.0	41.0
17	37.91205402099851	40.0	37.0	41.0	33.0	41.0
18	37.89094403861378	40.0	37.0	41.0	33.0	41.0
19	37.960394614778565	40.0	37.0	41.0	33.0	41.0
20	37.862437469573194	40.0	37.0	41.0	32.0	41.0
21	37.7302476907119	40.0	37.0	41.0	32.0	41.0
22	37.61497285283598	40.0	37.0	41.0	32.0	41.0
23	37.43191953531461	39.0	36.0	41.0	32.0	41.0
24	37.57758275933083	39.0	36.0	41.0	32.0	41.0
25	37.52232264598096	39.0	36.0	41.0	32.0	41.0
26	37.29853504191578	39.0	36.0	41.0	31.0	41.0
27	37.253050237840775	39.0	36.0	41.0	31.0	41.0
28	37.094144072370604	39.0	36.0	41.0	31.0	41.0
29	36.86196418123637	39.0	35.0	41.0	30.0	41.0
30	36.70680873923922	39.0	35.0	41.0	30.0	41.0
31	36.4293647859622	38.0	35.0	40.0	30.0	41.0
32	36.25635003057638	38.0	35.0	40.0	30.0	41.0
33	36.07411166329833	38.0	35.0	40.0	30.0	41.0
34	35.866031689850345	38.0	35.0	40.0	29.0	41.0
35	35.661841308908336	38.0	35.0	40.0	27.0	41.0
36	35.56853013583974	38.0	35.0	40.0	27.0	41.0
37	35.39997335981817	38.0	34.0	40.0	26.0	41.0
38	35.24819013658289	38.0	34.0	40.0	25.0	41.0
39	35.099714578477375	38.0	34.0	40.0	24.0	41.0
40	34.897498279094634	38.0	34.0	40.0	24.0	41.0
41	34.738652888507374	38.0	34.0	40.0	23.0	41.0
42	34.42107263574684	38.0	33.0	40.0	21.0	41.0
43	34.013731974579095	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	9.0
13	2.0
14	15.0
15	16.0
16	36.0
17	80.0
18	214.0
19	513.0
20	1111.0
21	2208.0
22	4238.0
23	7328.0
24	11866.0
25	18199.0
26	26807.0
27	37690.0
28	50832.0
29	66880.0
30	83799.0
31	101827.0
32	125158.0
33	154629.0
34	190324.0
35	235413.0
36	309541.0
37	455126.0
38	580565.0
39	711224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50801693131557	20.98005639153384	15.046318018272773	22.465608658877827
2	18.445901222236426	23.963032496613295	35.75317084291928	21.83789543823099
3	19.300150457197162	24.16544749522461	33.75408655980784	22.780315487770395
4	13.319366656443052	16.317363289577518	35.925954149916834	34.4373159040626
5	12.24251760424782	39.06949560625937	34.6125553980377	14.075431391455115
6	31.99511029854008	38.020672277269504	13.448096045727903	16.536121378462514
7	25.140900110654375	34.44682575620644	20.92337515359041	19.48889897954878
8	27.558701294284578	34.44622745425037	18.746752637409493	19.248318614055563
9	24.573835814607005	14.66781330711721	21.9454953215936	38.81285555668219
10	16.420397184328014	28.62525325492009	32.476271029526515	22.47807853122538
11	33.241971574988966	22.95476774232961	23.214241853803973	20.589018828877453
12	21.59315844862192	28.22876799550581	30.50461416766436	19.67345938820791
13	32.224133989408166	21.407842290123547	25.90830109325512	20.459722627213168
14	20.30495135805097	23.46672527926531	28.764972506450643	27.463350856233077
15	22.198640028164277	30.62178688232408	25.617179957262348	21.562393132249294
16	21.08617626479459	28.796556551815783	26.700925226740697	23.416341956648928
17	21.37336120370796	27.437781320005268	27.36050589894239	23.828351577344385
18	22.688397413572133	25.762126478514347	30.27152832141033	21.27794778650319
19	22.49102074722246	25.220505760388257	31.831616416649926	20.45685707573936
20	23.261035364683934	24.93042378042444	31.098224176815233	20.71031667807639
21	22.049536882796424	25.702674157827015	31.854792745053462	20.392996214323098
22	20.808910542521318	26.24753830234654	31.29157017735559	21.651980977776546
23	21.76339109991202	26.282743649024738	31.76662193047479	20.18724332058845
24	21.391656647733033	26.43335829407108	30.192237567442803	21.982747490753084
25	21.398836271205866	26.55868680907933	31.191433323655538	20.851043596059267
26	20.738405380435022	27.69237454710116	31.161549715428695	20.40767035703512
27	19.861609608603455	27.388940986644013	30.93523412815124	21.814215276601292
28	20.499147577160485	27.230233520402415	30.7377944826483	21.532824419788806
29	20.59188438035126	27.079366958742984	30.068420552113047	22.26032810879271
30	20.700082565669938	27.41910800106057	31.8637672743945	20.01704215887499
31	21.61255602782923	27.18734471702522	31.284170126846313	19.915929128299243
32	20.835393276471557	27.140015883342457	30.57704019392541	21.44755064626058
33	20.506201242326778	26.864135702441132	31.208973017841362	21.420690037390724
34	19.70828685996648	27.203750786452176	32.03116586378742	21.056796489793914
35	19.312305433778366	29.053574476312594	30.90509860331132	20.72902148659772
36	21.447519156683946	26.98093054218123	30.790193138169336	20.781357162965485
37	20.542130849267583	27.687462173146066	30.66716336225546	21.10324361533089
38	20.16882191825684	26.150676364616547	32.15841524297042	21.52208647415619
39	19.670719795040643	26.011618394195335	32.94105718066263	21.37660463010139
40	18.511745926980712	26.25065577043343	32.94445805493923	22.293140247646626
41	19.570268045574235	25.452552450613325	33.15669780146074	21.820481702351703
42	18.74650072079641	27.184731082164493	32.27026621917879	21.798501977860308
43	18.567199071435365	27.103456484868943	31.876016719705607	22.453327723990082
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1329.0
1	1169.5
2	1010.0
3	2310.5
4	3611.0
5	3611.0
6	5483.5
7	7356.0
8	8191.5
9	9027.0
10	13427.5
11	17828.0
12	17828.0
13	31725.5
14	45623.0
15	77867.5
16	110112.0
17	99829.0
18	89546.0
19	89546.0
20	89551.0
21	89556.0
22	71044.5
23	52533.0
24	58573.0
25	64613.0
26	64613.0
27	70195.0
28	75777.0
29	79393.0
30	83009.0
31	89796.0
32	96583.0
33	96583.0
34	105320.0
35	114057.0
36	125217.0
37	136377.0
38	142352.0
39	148327.0
40	148327.0
41	152801.0
42	157275.0
43	164450.5
44	171626.0
45	186576.5
46	201527.0
47	201527.0
48	263926.0
49	326325.0
50	301336.5
51	276348.0
52	249105.5
53	221863.0
54	221863.0
55	198379.0
56	174895.0
57	151958.0
58	129021.0
59	116138.0
60	103255.0
61	103255.0
62	91558.0
63	79861.0
64	70226.0
65	60591.0
66	52071.5
67	43552.0
68	43552.0
69	37138.0
70	30724.0
71	25870.5
72	21017.0
73	17223.0
74	13429.0
75	13429.0
76	10734.5
77	8040.0
78	6334.5
79	4629.0
80	3659.0
81	2689.0
82	2689.0
83	2035.5
84	1382.0
85	1041.5
86	701.0
87	527.5
88	354.0
89	354.0
90	268.0
91	182.0
92	120.5
93	59.0
94	42.0
95	25.0
96	25.0
97	14.5
98	4.0
99	5.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3175654.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.05176132163102
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.51816313444063	36.47366109892957
2	12.227049723241443	11.506084504913419
3	4.2827825809353435	6.045373913718259
4	1.9083823052882138	3.5917099495538007
5	1.0501934578675276	2.4706725960560636
6	0.6194090671647444	1.7486572553213804
7	0.42004532306070635	1.3834710599443827
8	0.3010980940052392	1.1333756522826026
9	0.20979907019324298	0.8884274198609323
>10	1.2569044181439046	11.118185967376066
>50	0.12168211363685036	4.007117726518616
>100	0.07339870953323581	6.6247749165384135
>500	0.005646837630791412	1.7773324104893347
>1k	0.004638473767982955	4.4244636134745745
>5k	5.377940600559948E-4	1.847551448207143
>10k+	2.688970300279974E-4	4.959140466815444
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	58517	1.8426755559642203	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40845	1.286191757666295	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	40584	1.2779729781644977	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16839	0.5302529809607721	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9752	0.3070863513468407	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8398	0.26444946458272844	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7781	0.24502039579878662	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7754	0.2441701772296352	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7331	0.23085008631292958	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6141	0.19337749011699637	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6043	0.19029151160674304	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5211	0.16409218384622506	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4841	0.15244104049118704	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4214	0.1326970759408928	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3878	0.12211657819145286	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3805	0.11981783909708048	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3768	0.11865272476157668	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3599	0.11333098631022145	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3266	0.10284495729068723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.148957663523797E-5	0.0
8	0.0	0.0	0.0	3.148957663523797E-5	0.0
9	0.0	0.0	3.148957663523797E-5	1.889374598114278E-4	0.0
10	3.148957663523797E-5	0.0	3.148957663523797E-5	2.834061897171417E-4	0.0
11	3.148957663523797E-5	0.0	3.148957663523797E-5	5.353228027990455E-4	0.0
12	6.297915327047594E-5	0.0	3.148957663523797E-5	8.502185691514252E-4	0.0
13	6.297915327047594E-5	0.0	3.148957663523797E-5	9.446872990571391E-4	0.0
14	6.297915327047594E-5	0.0	3.148957663523797E-5	0.0012280934887742808	0.0
15	6.297915327047594E-5	0.0	3.148957663523797E-5	0.0014170309485857086	0.0
16	6.297915327047594E-5	0.0	3.148957663523797E-5	0.001983843328019992	0.0
17	6.297915327047594E-5	0.0	3.148957663523797E-5	0.0028025723205361793	0.0
18	6.297915327047594E-5	0.0	3.148957663523797E-5	0.0034638534298761766	0.0
19	9.44687299057139E-5	0.0	3.148957663523797E-5	0.004219603269121888	0.0
20	9.44687299057139E-5	0.0	3.148957663523797E-5	0.007179623472834257	0.0
21	1.2595830654095187E-4	0.0	3.148957663523797E-5	0.012784768113906615	0.0
22	1.2595830654095187E-4	0.0	3.148957663523797E-5	0.020090349893281824	0.0
23	1.5744788317618985E-4	0.0	3.148957663523797E-5	0.02465633850539133	0.0
24	1.5744788317618985E-4	0.0	3.148957663523797E-5	0.030387441453004642	0.0
25	1.5744788317618985E-4	0.0	3.148957663523797E-5	0.034953430065114144	0.0
26	1.889374598114278E-4	0.0	3.148957663523797E-5	0.04336114702672268	0.0
27	1.889374598114278E-4	0.0	3.148957663523797E-5	0.09912918724772912	0.0
28	1.889374598114278E-4	0.0	3.148957663523797E-5	0.24139909448573427	0.0
29	1.889374598114278E-4	0.0	3.148957663523797E-5	0.4696985250912096	0.0
30	2.204270364466658E-4	0.0	3.148957663523797E-5	0.7770997722044026	0.0
31	2.204270364466658E-4	0.0	3.148957663523797E-5	1.2269598640154122	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14390	0.0	21.701181	1
GTATTGG	900	0.0	20.761112	1
ATTGGAC	945	0.0	19.380953	3
GGACCCT	1330	0.0	18.221804	6
TTGGACC	1310	0.0	17.935116	4
AACCGTC	85	2.7255639E-5	17.411764	7
ACGTTTA	195	1.8189894E-12	16.128206	26
TGGACCC	1455	0.0	16.020618	5
GCCGTCA	225	0.0	15.622222	13
GACCCTC	1445	0.0	15.491349	7
TATACAC	1930	0.0	15.336788	37
CGCGAAT	85	5.3678494E-4	15.235294	35
TAGTACT	500	0.0	15.17	4
CCGTATA	160	1.0984877E-8	15.031251	2
TCACGTT	210	9.094947E-12	14.9761915	24
TATACTG	370	0.0	14.5	5
TATTGGA	1220	0.0	14.405737	2
CCCGTAT	155	1.2121927E-7	14.322581	1
CTAATAC	470	0.0	14.170214	3
CTAGGCA	395	0.0	14.050632	4
>>END_MODULE