FastQCFastQC Report
Wed 25 May 2016
SRR2088550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6079753
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT637901.0492202561518535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT466850.7678765897232996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT460520.7574649825412315No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203420.3345859609757173No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA88690.14587763680531102No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG88100.14490720264458112No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG83890.1379825792264916No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA74000.12171547100679912No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG72400.11908378514719266No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA65950.10847480152565409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161000.022.0965841
GTATTGG15600.016.8397451
TATAGCG700.002594209315.8571425
TTTAGCG1557.226845E-915.51612926
GGGTAAG9950.015.2462311
TAATACT21300.015.1995314
TATACCG2700.015.0740755
TCTATAC6950.014.9064753
ATGCTAG16200.014.50308615
CGAGTTC8850.014.4237314
CGTTTAG5100.014.147058526
ATACTGG19650.014.1221376
TACTGGT19300.013.9948197
AATACTG21800.013.8325695
CTCTAAT18800.013.7765961
GTATCAA258650.013.7399972
CCGAGTT8800.013.66477313
GTATTAG19900.013.4798991
GGACCCT19150.013.4281986
CGAACTA12700.013.40157524
Produced by FastQC (version 0.11.5)