##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088550_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6079753
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74647572031298	31.0	31.0	34.0	30.0	34.0
2	31.905193187946946	31.0	31.0	34.0	30.0	34.0
3	32.04569659326621	33.0	31.0	34.0	30.0	34.0
4	35.711920533613785	37.0	35.0	37.0	35.0	37.0
5	35.61163636088506	37.0	35.0	37.0	33.0	37.0
6	35.647037305627386	37.0	35.0	37.0	33.0	37.0
7	35.52398362236097	37.0	35.0	37.0	33.0	37.0
8	35.539569617384124	37.0	35.0	37.0	33.0	37.0
9	37.16662634156355	39.0	37.0	39.0	33.0	39.0
10	37.01151230979285	39.0	37.0	39.0	33.0	39.0
11	37.071110454651695	39.0	37.0	39.0	33.0	39.0
12	36.936191486726514	39.0	37.0	39.0	33.0	39.0
13	36.994081667462474	39.0	37.0	39.0	33.0	39.0
14	38.080656566146686	40.0	37.0	41.0	33.0	41.0
15	38.07847045759918	40.0	37.0	41.0	33.0	41.0
16	37.97223308249529	40.0	37.0	41.0	33.0	41.0
17	37.958634339256875	40.0	37.0	41.0	33.0	41.0
18	37.91941087080347	40.0	37.0	41.0	33.0	41.0
19	37.973964896271276	40.0	37.0	41.0	33.0	41.0
20	37.868840724286	40.0	37.0	41.0	32.0	41.0
21	37.72527502350836	40.0	37.0	41.0	32.0	41.0
22	37.600271096539615	39.0	37.0	41.0	32.0	41.0
23	37.45488854563664	39.0	36.0	41.0	32.0	41.0
24	37.6386818675035	39.0	37.0	41.0	32.0	41.0
25	37.58463148091707	39.0	37.0	41.0	32.0	41.0
26	37.4155400721049	39.0	36.0	41.0	32.0	41.0
27	37.376056724672864	39.0	36.0	41.0	31.0	41.0
28	37.26094415348781	39.0	36.0	41.0	31.0	41.0
29	37.07433377638861	39.0	36.0	41.0	31.0	41.0
30	36.991035820040715	39.0	36.0	41.0	31.0	41.0
31	36.810849388124815	39.0	35.0	41.0	30.0	41.0
32	36.664277150732936	39.0	35.0	40.0	30.0	41.0
33	36.53306705058577	39.0	35.0	40.0	30.0	41.0
34	36.37685330308649	39.0	35.0	40.0	30.0	41.0
35	36.19779487752216	38.0	35.0	40.0	30.0	41.0
36	36.16629507810597	39.0	35.0	40.0	29.0	41.0
37	36.073497393726356	39.0	35.0	40.0	29.0	41.0
38	35.95927811541028	38.0	35.0	40.0	28.0	41.0
39	35.88395729234395	39.0	35.0	40.0	27.0	41.0
40	35.756085814670435	38.0	35.0	40.0	27.0	41.0
41	35.67582613964745	38.0	35.0	40.0	27.0	41.0
42	35.458135881507026	38.0	35.0	40.0	26.0	41.0
43	35.10875227990348	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	8.0
14	8.0
15	30.0
16	63.0
17	149.0
18	344.0
19	765.0
20	1703.0
21	3483.0
22	6504.0
23	11534.0
24	18661.0
25	28918.0
26	42586.0
27	60861.0
28	84107.0
29	111005.0
30	143451.0
31	179583.0
32	222384.0
33	276096.0
34	345678.0
35	433833.0
36	571956.0
37	823656.0
38	1204369.0
39	1508016.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50232747942228	21.05057557436955	13.367977284603501	24.079119661604672
2	18.171182283227623	23.17390196608316	35.884155162224516	22.770760588464693
3	18.67028150650199	24.733060701643637	32.57796821680091	24.018689575053457
4	13.010166695916759	16.588831816029366	37.828313091008795	32.57268839704508
5	12.490639011157196	38.57117221702921	35.65306024767783	13.285128524135766
6	31.552021932469955	39.0478856624603	14.52049121074491	14.879601194324835
7	26.517261474273706	32.93832825116415	22.09652267123352	18.44788760332862
8	26.466206768597345	35.156707846519424	19.673463708147352	18.703621676735878
9	25.193868895660724	14.45703468545515	21.2839732140434	39.065123204840724
10	15.733172054851572	28.078163701716168	34.29126150355121	21.897402739881045
11	33.54075404050131	22.946162451007467	23.310289085757265	20.202794422733948
12	20.989273741877344	27.59256831650891	30.927440637802228	20.490717303811522
13	30.332630289421296	21.1490499696287	26.00901714263721	22.509302598312793
14	20.610343874167256	22.36636916006292	28.18482921921335	28.83845774655648
15	23.120462295096527	29.618472987307214	25.17788140406362	22.08318331353264
16	21.972701851539036	28.57227916989391	26.176836460296986	23.278182518270068
17	21.522782257766064	27.432315095695497	27.4836165219212	23.561286124617233
18	22.054086736747365	26.62636130119102	29.402691194856107	21.91686076720551
19	22.790843641180818	25.640630466402172	30.21311885532192	21.35540703709509
20	23.162075827751554	25.944919143919172	29.780420355892744	21.11258467243653
21	22.393080771537925	26.35235345909612	29.845735509320853	21.408830260045104
22	21.593179854510534	26.909185290915598	29.749975040104427	21.747659814469436
23	22.17169019859853	26.752813806745106	30.056484202565464	21.019011792090897
24	22.233501920226033	26.75722188055995	28.628005117971078	22.38127108124294
25	22.273585785475166	26.633335268718977	29.513222000959576	21.57985694484628
26	21.66773880452051	27.29997419303054	29.4256691020178	21.606617900431154
27	21.194134038011082	27.362493180232818	29.32591175990209	22.117461021854012
28	21.504656521408023	27.086429333560098	29.470325521447993	21.938588623583886
29	21.82435700924034	27.257949459459947	29.20414694478542	21.713546586514287
30	21.793763661122416	27.30620799891048	29.807214207550864	21.092814132416233
31	22.050353032434046	27.34277198432239	29.546693755486448	21.060181227757113
32	21.35111409953661	27.069767472461464	29.392082211234566	22.187036216767357
33	21.184988929648952	26.858640474374536	30.027404073816815	21.928966522159698
34	21.238823353514526	26.881338764913643	30.390280657783304	21.489557223788534
35	20.976444273311763	28.223070904360753	29.378282308508258	21.42220251381923
36	22.26588810433582	27.204443996326827	29.014073433575344	21.515594465762014
37	21.408188786616826	27.077859906479752	29.449222690461273	22.064728616442146
38	21.18362374260928	26.440580727539427	30.261936628017615	22.113858901833677
39	20.72855591337345	26.485533211628827	30.86944486067115	21.91646601432657
40	20.144667061309892	26.60502819769158	30.917275751169498	22.333028989829025
41	20.881473309853213	25.603359215415495	31.092792749968623	22.422374724762665
42	19.978163586579914	26.971950998667214	30.642116546511016	22.407768868241853
43	20.168878571218272	26.16872757824208	30.49697907135372	23.16541477918593
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2811.0
1	2296.5
2	1782.0
3	3755.5
4	5729.0
5	5729.0
6	8198.0
7	10667.0
8	11182.5
9	11698.0
10	16881.5
11	22065.0
12	22065.0
13	38962.5
14	55860.0
15	90022.0
16	124184.0
17	116301.0
18	108418.0
19	108418.0
20	113661.5
21	118905.0
22	103329.0
23	87753.0
24	101702.5
25	115652.0
26	115652.0
27	131136.5
28	146621.0
29	161426.0
30	176231.0
31	197565.5
32	218900.0
33	218900.0
34	245508.0
35	272116.0
36	294500.0
37	316884.0
38	333388.5
39	349893.0
40	349893.0
41	359322.5
42	368752.0
43	387146.0
44	405540.0
45	405199.5
46	404859.0
47	404859.0
48	455562.0
49	506265.0
50	486174.5
51	466084.0
52	436409.0
53	406734.0
54	406734.0
55	378294.5
56	349855.0
57	311393.5
58	272932.0
59	248120.0
60	223308.0
61	223308.0
62	196560.5
63	169813.0
64	144968.5
65	120124.0
66	102267.5
67	84411.0
68	84411.0
69	70886.0
70	57361.0
71	48088.0
72	38815.0
73	31624.0
74	24433.0
75	24433.0
76	19726.5
77	15020.0
78	11775.5
79	8531.0
80	6932.5
81	5334.0
82	5334.0
83	4027.5
84	2721.0
85	2090.5
86	1460.0
87	1044.5
88	629.0
89	629.0
90	511.0
91	393.0
92	258.0
93	123.0
94	89.5
95	56.0
96	56.0
97	38.5
98	21.0
99	15.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	6079753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.20031641569554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.41885034860717	30.248798652392146
2	13.496809366308945	11.121456329885039
3	5.11963735475461	6.327920368485134
4	2.4811051043914447	4.0888926144609945
5	1.4115734674875422	2.9078636752243634
6	0.8329079085691717	2.0589641626911055
7	0.5790303277976175	1.6699362903682027
8	0.3849753262505672	1.2688884203007191
9	0.30589628680627984	1.1342721426124576
>10	1.664260361939202	12.954984044705997
>50	0.16779236895349472	4.833196686491528
>100	0.11957879735029853	9.477033868300664
>500	0.010413620548207754	2.999580197340739
>1k	0.006688732733881035	5.015534373665555
>5k	3.204183345536728E-4	0.9740441318298152
>10k+	1.602091672768364E-4	2.9186340412456606
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	63790	1.0492202561518535	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	46685	0.7678765897232996	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	46052	0.7574649825412315	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20342	0.3345859609757173	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8869	0.14587763680531102	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8810	0.14490720264458112	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8389	0.1379825792264916	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7400	0.12171547100679912	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7240	0.11908378514719266	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6595	0.10847480152565409	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.6448036622540422E-4	0.0
10	0.0	0.0	0.0	2.3027251271556592E-4	0.0
11	0.0	0.0	0.0	2.7961662258318715E-4	0.0
12	0.0	0.0	0.0	3.2896073245080844E-4	0.0
13	1.644803662254042E-5	0.0	0.0	3.2896073245080844E-4	0.0
14	1.644803662254042E-5	0.0	0.0	3.7830484231842973E-4	0.0
15	1.644803662254042E-5	0.0	0.0	4.4409698880859143E-4	0.0
16	3.289607324508084E-5	0.0	0.0	6.085773550339956E-4	0.0
17	3.289607324508084E-5	0.0	0.0	8.388498677495616E-4	0.0
18	3.289607324508084E-5	0.0	0.0	0.0010033302339749657	0.0
19	3.289607324508084E-5	0.0	0.0	0.0012500507833130721	0.0
20	3.289607324508084E-5	0.0	0.0	0.0022862770905331185	0.0
21	3.289607324508084E-5	0.0	0.0	0.004457417924708455	0.0
22	3.289607324508084E-5	0.0	0.0	0.0074509605900108114	0.0
23	3.289607324508084E-5	0.0	0.0	0.009013524069152151	0.0
24	3.289607324508084E-5	0.0	0.0	0.011793242258361482	0.0
25	3.289607324508084E-5	0.0	0.0	0.013915038982669197	0.0
26	3.289607324508084E-5	0.0	0.0	0.01756650311287317	0.0
27	3.289607324508084E-5	0.0	0.0	0.04692624848410783	0.0
28	3.289607324508084E-5	0.0	0.0	0.12872233460800134	0.0
29	3.289607324508084E-5	0.0	0.0	0.24683568559446412	0.0
30	3.289607324508084E-5	0.0	0.0	0.3966444031525623	0.0
31	3.289607324508084E-5	0.0	0.0	0.6538423518192269	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	16100	0.0	22.096584	1
GTATTGG	1560	0.0	16.839745	1
TATAGCG	70	0.0025942093	15.857142	5
TTTAGCG	155	7.226845E-9	15.516129	26
GGGTAAG	995	0.0	15.246231	1
TAATACT	2130	0.0	15.199531	4
TATACCG	270	0.0	15.074075	5
TCTATAC	695	0.0	14.906475	3
ATGCTAG	1620	0.0	14.503086	15
CGAGTTC	885	0.0	14.42373	14
CGTTTAG	510	0.0	14.1470585	26
ATACTGG	1965	0.0	14.122137	6
TACTGGT	1930	0.0	13.994819	7
AATACTG	2180	0.0	13.832569	5
CTCTAAT	1880	0.0	13.776596	1
GTATCAA	25865	0.0	13.739997	2
CCGAGTT	880	0.0	13.664773	13
GTATTAG	1990	0.0	13.479899	1
GGACCCT	1915	0.0	13.428198	6
CGAACTA	1270	0.0	13.401575	24
>>END_MODULE