FastQCFastQC Report
Wed 25 May 2016
SRR2088549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2955005
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT397521.3452430706547027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT286880.9708274605288315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT265350.8979680237427685No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121220.41021927204860903No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA60480.2046697044505847No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG60430.20450049999915396No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG51680.17488972099878003No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA50100.16954286033356966No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG47050.15922138879629646No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA45660.15451750504652276No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA37880.12818929240390456No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37030.12531281672958253No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC31480.10653112262077391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG7350.019.8843541
GGTATCA108050.019.7584461
ACCGTTA1800.018.58
ATTGGAC8500.016.9764693
GGACCGT2400.016.18756
GGACCCT11450.015.9956326
TTGGACC11550.015.8571424
ACGTTTA1557.221388E-915.516129526
GTATTAG5950.015.2352931
CGAACTA3200.015.0312524
TGTTACG1001.0942869E-414.816
GGGTAAG3700.014.51
GACCCTC12400.014.4717757
TGGACCC11900.014.4579835
GACCGTT1803.3360266E-914.3888887
GTATACT2850.014.2807024
CCCTCGC11250.014.1422229
CCTCGCA11100.014.010
TCGCATG11800.013.9533912
TCTATAC2304.0017767E-1113.6739133
Produced by FastQC (version 0.11.5)