##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088549_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2955005
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.472081434718383	31.0	31.0	34.0	30.0	34.0
2	31.63776372628811	31.0	31.0	34.0	30.0	34.0
3	31.762849132234972	31.0	31.0	34.0	30.0	34.0
4	35.48423843614478	37.0	35.0	37.0	33.0	37.0
5	35.36975571953347	37.0	35.0	37.0	33.0	37.0
6	35.42418168497177	37.0	35.0	37.0	33.0	37.0
7	35.27927837685554	37.0	35.0	37.0	32.0	37.0
8	35.322790655176554	37.0	35.0	37.0	32.0	37.0
9	36.88776804100162	39.0	37.0	39.0	33.0	39.0
10	36.69349019714011	39.0	35.0	39.0	32.0	39.0
11	36.77307314200822	39.0	37.0	39.0	32.0	39.0
12	36.60222740739864	38.0	35.0	39.0	32.0	39.0
13	36.67487263134919	39.0	35.0	39.0	32.0	39.0
14	37.65094475305456	39.0	37.0	41.0	32.0	41.0
15	37.65733425154949	39.0	37.0	41.0	32.0	41.0
16	37.53627354268436	39.0	36.0	41.0	32.0	41.0
17	37.540439694687485	39.0	36.0	41.0	32.0	41.0
18	37.49771557070123	39.0	36.0	41.0	32.0	41.0
19	37.557655232393856	39.0	37.0	41.0	32.0	41.0
20	37.44735017368837	39.0	36.0	41.0	32.0	41.0
21	37.30049390779373	39.0	36.0	41.0	31.0	41.0
22	37.174887013727556	39.0	36.0	40.0	31.0	41.0
23	36.99449916328399	39.0	36.0	40.0	31.0	41.0
24	37.1674291583263	39.0	36.0	41.0	31.0	41.0
25	37.10479948426483	39.0	36.0	41.0	31.0	41.0
26	36.88025773222042	39.0	36.0	40.0	30.0	41.0
27	36.832069658088564	39.0	36.0	40.0	30.0	41.0
28	36.68098497295267	39.0	35.0	40.0	30.0	41.0
29	36.46029093013379	39.0	35.0	40.0	30.0	41.0
30	36.34295779533368	38.0	35.0	40.0	30.0	41.0
31	36.104890854668604	38.0	35.0	40.0	29.0	41.0
32	35.945041378948595	38.0	35.0	40.0	29.0	41.0
33	35.779977022035496	38.0	35.0	40.0	28.0	41.0
34	35.58401187138431	38.0	34.0	40.0	27.0	41.0
35	35.37009345161852	38.0	34.0	40.0	26.0	41.0
36	35.32936255607012	38.0	34.0	40.0	26.0	41.0
37	35.19440880810692	38.0	34.0	40.0	25.0	41.0
38	35.06351461334245	38.0	34.0	40.0	25.0	41.0
39	34.94336760851505	38.0	34.0	40.0	24.0	41.0
40	34.77922372381773	38.0	33.0	40.0	24.0	41.0
41	34.66115015033815	38.0	33.0	40.0	23.0	41.0
42	34.392632161366905	38.0	33.0	40.0	22.0	41.0
43	34.00633806034169	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	1.0
13	4.0
14	11.0
15	17.0
16	50.0
17	123.0
18	272.0
19	643.0
20	1390.0
21	2727.0
22	4934.0
23	8552.0
24	13258.0
25	20029.0
26	28611.0
27	39273.0
28	52622.0
29	68036.0
30	85067.0
31	103394.0
32	126370.0
33	153269.0
34	188996.0
35	232404.0
36	305507.0
37	428392.0
38	587131.0
39	503920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.91485462799555	21.609032810435178	14.196388838597565	23.279723722971703
2	19.06626215522478	23.96290361606833	34.50904482395123	22.46178940475566
3	19.884974813917406	24.70879067886518	32.53216153610569	22.874072971111726
4	14.164578401728592	16.8677548768953	36.17103862768422	32.796628093691886
5	12.750096869548443	38.921355463019516	34.7525638704503	13.575983796981731
6	32.426341072180925	38.09293046881477	13.861262502093904	15.619465956910394
7	26.257823590823026	33.36545961851164	21.490894262446254	18.885822528219073
8	27.497652288236402	34.71997509310475	18.925213324512143	18.85715929414671
9	25.155727316874255	14.429654095339941	21.18108091187663	39.23353767590918
10	16.22819589137751	28.34117031950877	33.27222796577332	22.1584058233404
11	34.09127903336881	22.841213466643882	22.796441968795314	20.271065531191994
12	21.3100823856474	27.391459574518485	30.800692384615253	20.497765655218856
13	31.59358444401955	20.908966313085763	25.802122162229846	21.69532708066484
14	20.69945736132426	22.400063620873738	28.062118338209242	28.838360679592757
15	23.097287483439114	29.914230263569774	24.72564344222768	22.262838810763434
16	22.096849243909908	28.20915700650253	26.208043641212114	23.48595010837545
17	22.05387131324651	27.167534403495086	27.17985248755924	23.59874179569916
18	22.911500995768197	25.924558503285105	29.21900301353128	21.94493748741542
19	22.958066060801926	25.24709772064683	30.48515992358727	21.309676294963968
20	23.490924719247513	25.18865450312267	29.85639618207076	21.46402459555906
21	22.602973599029443	25.78997328261712	30.279508833318385	21.32754428503505
22	21.792315072224923	26.26956637975232	29.830846309904718	22.10727223811804
23	22.44067268921711	26.17728227194201	30.207867668582626	21.174177370258256
24	22.196612188473456	26.332138185891395	28.908479004265647	22.562770621369506
25	22.32405698129106	26.230581674142684	29.753519875600887	21.691841468965364
26	21.83806795589178	27.101172417643966	29.788748242388763	21.272011384075494
27	21.293974121871198	26.97646873693953	29.458494994086305	22.271062147102967
28	21.64270449626989	26.876096656350835	29.530169999712353	21.951028847666926
29	21.881113568335756	26.841409743807542	28.950306344659317	22.327170343197388
30	21.769540153062348	27.08289833688945	30.0579863655053	21.089575144542902
31	22.365545912781872	26.862492618455807	29.834230398933336	20.937731069828985
32	21.70585159754383	26.821748186551293	29.17138888089868	22.3010113350062
33	21.572586171597003	26.60344060331539	29.754704306760903	22.069268918326703
34	21.197933675239128	26.933592328946993	30.238392151620726	21.630081844193157
35	20.876479058411068	28.369089053994834	29.41714142615664	21.33729046143746
36	22.440706530107395	26.758465721716206	29.26539887411358	21.535428874062816
37	21.455801259219527	27.163811905563612	29.429459510220795	21.950927324996066
38	21.351402112686777	26.160869440153235	30.40350185532681	22.08422659183318
39	20.99048901778508	25.973763157761155	31.00624195221328	22.029505872240488
40	20.239018208091018	26.3856067925435	30.949829188106282	22.425545811259205
41	21.01458373166881	25.415794558723253	31.15998111678322	22.40964059282472
42	20.05999989847733	26.906993389182084	30.539779120509102	22.493227591831484
43	20.185549601438915	26.539616684235728	30.262486865504457	23.0123468488209
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	800.0
1	720.5
2	641.0
3	1400.5
4	2160.0
5	2160.0
6	3593.5
7	5027.0
8	5392.0
9	5757.0
10	8656.0
11	11555.0
12	11555.0
13	21568.5
14	31582.0
15	53566.5
16	75551.0
17	68475.0
18	61399.0
19	61399.0
20	63491.5
21	65584.0
22	53005.0
23	40426.0
24	46110.5
25	51795.0
26	51795.0
27	58960.0
28	66125.0
29	71674.5
30	77224.0
31	84957.0
32	92690.0
33	92690.0
34	103894.5
35	115099.0
36	125135.0
37	135171.0
38	143716.5
39	152262.0
40	152262.0
41	158219.0
42	164176.0
43	173225.5
44	182275.0
45	189324.5
46	196374.0
47	196374.0
48	235468.0
49	274562.0
50	260198.0
51	245834.0
52	227211.5
53	208589.0
54	208589.0
55	189682.0
56	170775.0
57	151251.0
58	131727.0
59	119961.0
60	108195.0
61	108195.0
62	96785.0
63	85375.0
64	74402.5
65	63430.0
66	54303.0
67	45176.0
68	45176.0
69	38625.0
70	32074.0
71	27186.0
72	22298.0
73	18353.5
74	14409.0
75	14409.0
76	11545.0
77	8681.0
78	6761.0
79	4841.0
80	3728.0
81	2615.0
82	2615.0
83	1978.0
84	1341.0
85	1022.0
86	703.0
87	531.0
88	359.0
89	359.0
90	286.0
91	213.0
92	147.0
93	81.0
94	57.5
95	34.0
96	34.0
97	24.0
98	14.0
99	10.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2955005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.996020617238436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.84976010464501	38.63324471751492
2	11.53350754728855	11.301919471520499
3	3.777398047598673	5.552324178589823
4	1.7484667188943037	3.4267164563000216
5	0.9627537993784405	2.358555250183536
6	0.6099982324088425	1.7932491582949592
7	0.38328871262493497	1.3145735166288266
8	0.29407741905488033	1.1526898629661748
9	0.22372091724990997	0.9865291206675677
>10	1.395338956766668	12.923755000987866
>50	0.1350491369228344	4.603297275977702
>100	0.07819547991873917	7.111504034229831
>500	0.004707008630220569	1.5474238517999197
>1k	0.0031841528968906533	2.906719002712186
>5k	2.7688286059918723E-4	0.7552619505442818
>10k+	2.7688286059918723E-4	3.632237151081815
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39752	1.3452430706547027	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28688	0.9708274605288315	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26535	0.8979680237427685	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12122	0.41021927204860903	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6048	0.2046697044505847	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6043	0.20450049999915396	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5168	0.17488972099878003	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5010	0.16954286033356966	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4705	0.15922138879629646	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4566	0.15451750504652276	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3788	0.12818929240390456	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3703	0.12531281672958253	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3148	0.10653112262077391	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.76817805722833E-5	0.0
10	0.0	0.0	0.0	6.76817805722833E-5	0.0
11	0.0	0.0	0.0	1.6920445143070825E-4	0.0
12	0.0	0.0	0.0	1.6920445143070825E-4	0.0
13	0.0	0.0	0.0	1.6920445143070825E-4	0.0
14	0.0	0.0	0.0	2.707271222891332E-4	0.0
15	0.0	0.0	0.0	3.384089028614165E-4	0.0
16	0.0	0.0	0.0	7.106586960089746E-4	0.0
17	0.0	0.0	0.0	9.813858182981079E-4	0.0
18	0.0	0.0	0.0	0.0010829084891565328	0.0
19	0.0	0.0	0.0	0.0013874765017318075	0.0
20	0.0	0.0	0.0	0.0025380667714606235	0.0
21	3.384089028614165E-5	0.0	0.0	0.004974610872062822	0.0
22	3.384089028614165E-5	0.0	0.0	0.007749563875526437	0.0
23	3.384089028614165E-5	0.0	0.0	0.00944160838983352	0.0
24	3.384089028614165E-5	0.0	0.0	0.012690333857303117	0.0
25	3.384089028614165E-5	0.0	0.0	0.015296082409336024	0.0
26	3.384089028614165E-5	0.0	0.0	0.020778306635690972	6.76817805722833E-5
27	3.384089028614165E-5	0.0	0.0	0.05587130986241986	6.76817805722833E-5
28	3.384089028614165E-5	0.0	0.0	0.14429755618010798	6.76817805722833E-5
29	3.384089028614165E-5	0.0	0.0	0.276175505625202	6.76817805722833E-5
30	3.384089028614165E-5	0.0	0.0	0.44514307082390725	6.76817805722833E-5
31	3.384089028614165E-5	0.0	0.0	0.7172576696147722	6.76817805722833E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	735	0.0	19.884354	1
GGTATCA	10805	0.0	19.758446	1
ACCGTTA	180	0.0	18.5	8
ATTGGAC	850	0.0	16.976469	3
GGACCGT	240	0.0	16.1875	6
GGACCCT	1145	0.0	15.995632	6
TTGGACC	1155	0.0	15.857142	4
ACGTTTA	155	7.221388E-9	15.5161295	26
GTATTAG	595	0.0	15.235293	1
CGAACTA	320	0.0	15.03125	24
TGTTACG	100	1.0942869E-4	14.8	16
GGGTAAG	370	0.0	14.5	1
GACCCTC	1240	0.0	14.471775	7
TGGACCC	1190	0.0	14.457983	5
GACCGTT	180	3.3360266E-9	14.388888	7
GTATACT	285	0.0	14.280702	4
CCCTCGC	1125	0.0	14.142222	9
CCTCGCA	1110	0.0	14.0	10
TCGCATG	1180	0.0	13.95339	12
TCTATAC	230	4.0017767E-11	13.673913	3
>>END_MODULE