FastQCFastQC Report
Wed 25 May 2016
SRR2088548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5013916
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT885531.7661444667202244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT653461.3032926758246448No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT647511.291425703980681No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272210.542908975738724No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA195030.3889773981055925No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG192420.38377188608664364No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG166290.3316569324256729No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA143810.28682171779503285No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA142380.2839696556543827No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG137350.27393757693587206No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA105910.21123209882255703No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA91040.1815746414578944No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC88030.17557134981918326No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA81540.1626273754885403No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT80640.16083237134407516No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG68340.13630064803638514No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC65730.13109513601743628No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA62260.12417439781599851No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61220.12210017080461659No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC58200.1160769346754114No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG55120.10993403160324186No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC54650.10899664055002119No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA53480.10666313516221651No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA52550.10480829754626923No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT52340.10438946324589403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG21650.021.7043881
GGTATCA220800.021.1895391
ATTGGAC25350.019.3392493
TTGGACC32300.017.9272444
GGACCCT32450.017.5593226
TGGACCC32000.017.5171895
CTAATAC10650.017.0234743
CTCTAAT7100.016.154931
TATTGGA30150.015.7694862
ACGTTTA2300.015.28260926
GACCCTC37300.015.1273477
TCTAATA9600.015.0312492
AATACTG11100.015.05
GAACCGT2109.094947E-1214.976196
TATACCG3100.014.9193545
CCGTTTA3000.014.827
TCGGTTC1304.4508542E-614.23076913
TAATACT10650.014.0704224
ACCGTAT1203.3047123E-513.8749998
ATACTGG9750.013.6615386
Produced by FastQC (version 0.11.5)