##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088548_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5013916
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74285189460693	31.0	31.0	34.0	30.0	34.0
2	31.8840890034855	31.0	31.0	34.0	30.0	34.0
3	32.00703003400935	33.0	31.0	34.0	30.0	34.0
4	35.66136468979536	37.0	35.0	37.0	33.0	37.0
5	35.592577338750786	37.0	35.0	37.0	33.0	37.0
6	35.6487671512646	37.0	35.0	37.0	33.0	37.0
7	35.499291172807844	37.0	35.0	37.0	33.0	37.0
8	35.549271667096136	37.0	35.0	37.0	33.0	37.0
9	37.14548069812099	39.0	37.0	39.0	33.0	39.0
10	37.01909485519901	39.0	37.0	39.0	33.0	39.0
11	37.07742969766546	39.0	37.0	39.0	33.0	39.0
12	36.92944476931803	39.0	37.0	39.0	33.0	39.0
13	36.98706141068179	39.0	37.0	39.0	33.0	39.0
14	38.004255157046906	40.0	37.0	41.0	33.0	41.0
15	38.012010771620425	40.0	37.0	41.0	33.0	41.0
16	37.90271715760695	40.0	37.0	41.0	33.0	41.0
17	37.95123731630127	40.0	37.0	41.0	33.0	41.0
18	37.92311618303936	40.0	37.0	41.0	33.0	41.0
19	37.990873600594824	40.0	37.0	41.0	33.0	41.0
20	37.90250574600771	40.0	37.0	41.0	32.0	41.0
21	37.76270603655905	40.0	37.0	41.0	32.0	41.0
22	37.649685395606944	40.0	37.0	41.0	32.0	41.0
23	37.46217607155764	39.0	36.0	41.0	32.0	41.0
24	37.6085193688925	39.0	37.0	41.0	32.0	41.0
25	37.55874031395819	40.0	36.0	41.0	32.0	41.0
26	37.34900744248607	39.0	36.0	41.0	31.0	41.0
27	37.314043753425466	39.0	36.0	41.0	31.0	41.0
28	37.169384967757736	39.0	36.0	41.0	31.0	41.0
29	36.95229776486084	39.0	35.0	41.0	31.0	41.0
30	36.8052689753877	39.0	35.0	41.0	30.0	41.0
31	36.529722675848575	39.0	35.0	40.0	30.0	41.0
32	36.369708626949475	38.0	35.0	40.0	30.0	41.0
33	36.185860712465065	38.0	35.0	40.0	30.0	41.0
34	35.97896215253706	38.0	35.0	40.0	29.0	41.0
35	35.7532738881146	38.0	35.0	40.0	28.0	41.0
36	35.67276875001496	38.0	35.0	40.0	27.0	41.0
37	35.50849076849313	38.0	35.0	40.0	26.0	41.0
38	35.360850879831254	38.0	34.0	40.0	25.0	41.0
39	35.229678957525415	38.0	34.0	40.0	25.0	41.0
40	35.02485043626579	38.0	34.0	40.0	24.0	41.0
41	34.873020609040914	38.0	34.0	40.0	23.0	41.0
42	34.55078625170426	38.0	33.0	40.0	22.0	41.0
43	34.1675588502081	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	9.0
13	6.0
14	11.0
15	33.0
16	58.0
17	138.0
18	294.0
19	799.0
20	1631.0
21	3404.0
22	6154.0
23	11090.0
24	17951.0
25	27885.0
26	40841.0
27	58000.0
28	79272.0
29	103156.0
30	130197.0
31	157615.0
32	192005.0
33	237321.0
34	294015.0
35	363956.0
36	481215.0
37	719050.0
38	941068.0
39	1146737.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.44199543829613	20.6392967093984	14.226784014730203	22.691923837575263
2	18.148608792010076	23.85006051158416	35.88243201521525	22.11889868119051
3	19.441111498477436	24.407429242931077	33.55616647745993	22.595292781131555
4	12.840941092750658	15.774915255859892	36.72488729368422	34.65925635770524
5	12.26833477066628	38.85404143188677	34.94386423705543	13.933759560391518
6	31.945628925574344	37.53624911147295	13.366957085040914	17.151164877911796
7	25.915511947148694	33.58612310218201	21.06343624424502	19.43492870642428
8	27.81911384235396	33.83271678265053	18.778475746302888	19.569693628692622
9	24.470812833721187	14.358696874857896	21.9867065981959	39.18378369322502
10	16.221432509040838	27.80575103372294	33.11617107267054	22.856645384565677
11	32.88686926546037	22.42624327970393	23.942363613590654	20.744523841245048
12	21.74713337838129	27.33496133561073	30.99459584085573	19.923309445152253
13	32.37946148280107	20.51436442094363	26.53825871833513	20.567915377920173
14	20.54984566953256	22.518546381710426	28.72367626422142	28.207931684535602
15	22.404503785065405	29.555600851709524	26.001053866877704	22.038841496347366
16	21.395671566895018	28.220456824565872	26.86116001943391	23.522711589105203
17	21.569228523174303	26.385623532584113	27.335539725835055	24.70960821840653
18	23.107547074980914	24.900816048773056	30.442652010923197	21.548984865322833
19	22.578519464626055	23.843797941569026	32.0729744973789	21.50470809642603
20	23.71126680223602	23.745571325885795	31.498852394016975	21.044309477861216
21	22.40986885300831	24.71176222337989	32.31637705936837	20.56199186424344
22	20.862076668217018	25.195834952161146	31.787887950256845	22.15420042936499
23	22.155696266152045	25.285226158555506	32.506567720719694	20.052509854572754
24	21.761174299689106	25.29360284456301	30.188379701614465	22.756843154133417
25	21.92643434792286	25.553599222643538	31.35577061921261	21.164195810220992
26	21.50389037231577	26.483610814381414	31.265741189122437	20.746757624180383
27	20.080013306964055	26.6675588502081	31.10638471007492	22.146043132752922
28	20.95013159374828	26.058912833800967	30.940047659354487	22.05090791309627
29	21.085654406655397	26.13897001864411	30.271368726560237	22.504006848140257
30	21.0826228441003	26.410913146530575	32.093078543796906	20.41338546557222
31	22.15890732912159	26.08170938643567	31.73585277455785	20.02353050988489
32	21.56557868141389	26.010746889257817	30.495405188279978	21.928269241048316
33	21.169899934502293	25.975923808855196	31.26542207727453	21.58875417936798
34	20.29609191697667	26.875240829722713	31.70641470658862	21.12225254671199
35	19.79333120060248	29.31901930546902	30.34302928090538	20.544620213023116
36	22.292415748488807	26.73377455864837	30.23700436943898	20.736805323423845
37	21.29730533977833	27.61027508239069	30.07812655816332	21.014293019667658
38	20.98768706934859	26.113102014473316	31.339495914969458	21.559715001208637
39	20.563328145106542	25.805418359621502	32.17361439641191	21.457639098860053
40	19.263186698779954	26.540731835156393	31.703662366900442	22.492419099163207
41	21.01764369407066	25.413648732846738	31.79075995688799	21.777947616194606
42	19.59608018961626	27.598308388094257	31.03711749458906	21.768493927700426
43	19.88439774419835	27.476008772384695	30.138578308850803	22.501015174566145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	845.0
1	957.0
2	1069.0
3	3201.0
4	5333.0
5	5333.0
6	8291.5
7	11250.0
8	11909.0
9	12568.0
10	18853.5
11	25139.0
12	25139.0
13	45827.5
14	66516.0
15	113230.5
16	159945.0
17	145061.0
18	130177.0
19	130177.0
20	130999.5
21	131822.0
22	100731.0
23	69640.0
24	77007.0
25	84374.0
26	84374.0
27	95124.5
28	105875.0
29	112893.0
30	119911.0
31	128986.0
32	138061.0
33	138061.0
34	153379.5
35	168698.0
36	186558.0
37	204418.0
38	218752.0
39	233086.0
40	233086.0
41	242398.0
42	251710.0
43	267578.0
44	283446.0
45	306013.5
46	328581.0
47	328581.0
48	439219.0
49	549857.0
50	512740.5
51	475624.0
52	431152.5
53	386681.0
54	386681.0
55	340927.5
56	295174.0
57	251148.0
58	207122.0
59	184529.0
60	161936.0
61	161936.0
62	143025.5
63	124115.0
64	108239.0
65	92363.0
66	78345.5
67	64328.0
68	64328.0
69	54872.5
70	45417.0
71	38601.5
72	31786.0
73	26073.5
74	20361.0
75	20361.0
76	16164.0
77	11967.0
78	9401.0
79	6835.0
80	5313.0
81	3791.0
82	3791.0
83	2893.0
84	1995.0
85	1530.0
86	1065.0
87	787.5
88	510.0
89	510.0
90	427.0
91	344.0
92	222.5
93	101.0
94	81.5
95	62.0
96	62.0
97	37.5
98	13.0
99	9.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5013916.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.077332185087585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34560063897399	29.05227732669046
2	13.014459471260038	10.171407119355756
3	4.870460290177066	5.709737839605818
4	2.2698988663451036	3.548063681068854
5	1.3259268744224613	2.590684246247071
6	0.8096044325939667	1.8982308810596273
7	0.5490213334811738	1.5018002317600503
8	0.39340084845254086	1.229844450950019
9	0.2880427692100404	1.013034867833994
>10	1.81551457737362	13.618215040009996
>50	0.1845567734800683	5.029033374676695
>100	0.11746657912508406	8.576378793077893
>500	0.00917677844463797	2.4468814029568016
>1k	0.0055880937759146265	4.665257691023999
>5k	7.177368152550871E-4	1.8534016834602718
>10k+	5.63936069128997E-4	7.095751370222817
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	88553	1.7661444667202244	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	65346	1.3032926758246448	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	64751	1.291425703980681	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27221	0.542908975738724	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	19503	0.3889773981055925	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	19242	0.38377188608664364	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	16629	0.3316569324256729	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	14381	0.28682171779503285	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	14238	0.2839696556543827	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	13735	0.27393757693587206	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	10591	0.21123209882255703	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	9104	0.1815746414578944	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	8803	0.17557134981918326	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	8154	0.1626273754885403	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8064	0.16083237134407516	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	6834	0.13630064803638514	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	6573	0.13109513601743628	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6226	0.12417439781599851	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6122	0.12210017080461659	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	5820	0.1160769346754114	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	5512	0.10993403160324186	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	5465	0.10899664055002119	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5348	0.10666313516221651	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5255	0.10480829754626923	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	5234	0.10438946324589403	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	1.994449049405694E-5	0.0	0.0	0.0	0.0
4	1.994449049405694E-5	0.0	0.0	0.0	0.0
5	7.977796197622777E-5	0.0	0.0	0.0	0.0
6	9.97224524702847E-5	0.0	0.0	0.0	0.0
7	9.97224524702847E-5	0.0	0.0	0.0	0.0
8	1.1966694296434165E-4	0.0	0.0	1.994449049405694E-5	0.0
9	1.3961143345839858E-4	0.0	0.0	5.9833471482170825E-5	0.0
10	1.994449049405694E-4	0.0	0.0	7.977796197622777E-5	0.0
11	1.994449049405694E-4	0.0	0.0	1.3961143345839858E-4	0.0
12	1.994449049405694E-4	0.0	0.0	1.7950041444651245E-4	0.0
13	1.994449049405694E-4	0.0	0.0	1.7950041444651245E-4	0.0
14	2.1938939543462635E-4	0.0	0.0	2.5927837642274025E-4	0.0
15	2.1938939543462635E-4	0.0	0.0	3.1911184790491107E-4	0.0
16	2.1938939543462635E-4	0.0	0.0	5.783902243276513E-4	0.0
17	2.1938939543462635E-4	0.0	0.0	0.0010969469771731318	0.0
18	2.1938939543462635E-4	0.0	0.0	0.0015157812775483275	0.0
19	2.1938939543462635E-4	0.0	0.0	0.002353449878298719	0.0
20	2.393338859286833E-4	0.0	0.0	0.0038891756463411034	0.0
21	2.5927837642274025E-4	0.0	0.0	0.008177241102563345	0.0
22	2.5927837642274025E-4	0.0	1.994449049405694E-5	0.013642031497934947	0.0
23	2.5927837642274025E-4	0.0	1.994449049405694E-5	0.018269153292556156	0.0
24	2.5927837642274025E-4	0.0	1.994449049405694E-5	0.024491834326701923	0.0
25	2.7922286691679717E-4	0.0	1.994449049405694E-5	0.029557734912192386	0.0
26	2.991673574108541E-4	0.0	1.994449049405694E-5	0.03685741843301723	0.0
27	2.991673574108541E-4	0.0	1.994449049405694E-5	0.06888827016647267	0.0
28	2.991673574108541E-4	0.0	1.994449049405694E-5	0.1587780888231873	0.0
29	3.1911184790491107E-4	0.0	1.994449049405694E-5	0.29813024390516313	0.0
30	3.1911184790491107E-4	0.0	1.994449049405694E-5	0.4849103973820064	0.0
31	3.1911184790491107E-4	0.0	1.994449049405694E-5	0.7344359179531528	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	2165	0.0	21.704388	1
GGTATCA	22080	0.0	21.189539	1
ATTGGAC	2535	0.0	19.339249	3
TTGGACC	3230	0.0	17.927244	4
GGACCCT	3245	0.0	17.559322	6
TGGACCC	3200	0.0	17.517189	5
CTAATAC	1065	0.0	17.023474	3
CTCTAAT	710	0.0	16.15493	1
TATTGGA	3015	0.0	15.769486	2
ACGTTTA	230	0.0	15.282609	26
GACCCTC	3730	0.0	15.127347	7
TCTAATA	960	0.0	15.031249	2
AATACTG	1110	0.0	15.0	5
GAACCGT	210	9.094947E-12	14.97619	6
TATACCG	310	0.0	14.919354	5
CCGTTTA	300	0.0	14.8	27
TCGGTTC	130	4.4508542E-6	14.230769	13
TAATACT	1065	0.0	14.070422	4
ACCGTAT	120	3.3047123E-5	13.874999	8
ATACTGG	975	0.0	13.661538	6
>>END_MODULE