FastQCFastQC Report
Wed 25 May 2016
SRR2088547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5520566
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT806321.4605748758370067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT575301.0421032915827835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT564171.021942315335058No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254270.46058683113289467No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG126070.228364265548134No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA123550.22379951620902638No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG117100.2121159315910724No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA103240.18700981022598046No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG102160.18505348908064861No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA91190.16518233818778727No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA77070.1396052506210414No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT71900.13024026884199916No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC68640.12433507723664566No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA64130.11616562504641734No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59470.10772446158600404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203900.019.9244751
ATTGGAC18750.017.7599983
GTATTGG19350.017.2093031
TTGGACC23450.017.0405124
GCGTAAG855.3687405E-415.2352941
TGGACCC26150.014.6443595
GGACCCT25150.014.6381716
TATTGGA23100.014.5757582
GTATTAG12350.014.3805681
TATACCG4350.014.0344835
TATACAC22100.013.97963837
CTAATAC11750.013.8553193
ACGTTCG5100.013.78431322
TAGGTCG3100.013.72580621
CTAGTAC8050.013.5590073
GACCCTC25900.013.57
TAGTACT8550.013.4152054
CGTTCGC5350.013.14018623
CTGTGCG5650.012.7699129
TATACTG8550.012.7660825
Produced by FastQC (version 0.11.5)