##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088547_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5520566
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76661994440425	31.0	31.0	34.0	30.0	34.0
2	31.91831725225276	31.0	31.0	34.0	30.0	34.0
3	32.04836370038869	33.0	31.0	34.0	30.0	34.0
4	35.70823172841335	37.0	35.0	37.0	35.0	37.0
5	35.62113993384012	37.0	35.0	37.0	33.0	37.0
6	35.66788441619936	37.0	35.0	37.0	33.0	37.0
7	35.52971380108489	37.0	35.0	37.0	33.0	37.0
8	35.56437238500545	37.0	35.0	37.0	33.0	37.0
9	37.17769735929251	39.0	37.0	39.0	33.0	39.0
10	37.035735828536424	39.0	37.0	39.0	33.0	39.0
11	37.101085830692	39.0	37.0	39.0	33.0	39.0
12	36.958454259943636	39.0	37.0	39.0	33.0	39.0
13	37.01633691183114	39.0	37.0	39.0	33.0	39.0
14	38.065597621693136	40.0	37.0	41.0	33.0	41.0
15	38.07381815560216	40.0	37.0	41.0	33.0	41.0
16	37.96511643914773	40.0	37.0	41.0	33.0	41.0
17	37.986006688444625	40.0	37.0	41.0	33.0	41.0
18	37.95276589393189	40.0	37.0	41.0	33.0	41.0
19	38.01335859402822	40.0	37.0	41.0	33.0	41.0
20	37.91479551190947	40.0	37.0	41.0	32.0	41.0
21	37.78020097939233	40.0	37.0	41.0	32.0	41.0
22	37.666595055651904	40.0	37.0	41.0	32.0	41.0
23	37.50126581223737	39.0	36.0	41.0	32.0	41.0
24	37.65694151650392	40.0	37.0	41.0	32.0	41.0
25	37.60769294307866	40.0	37.0	41.0	32.0	41.0
26	37.40812590593066	39.0	36.0	41.0	32.0	41.0
27	37.37030895020547	39.0	36.0	41.0	32.0	41.0
28	37.23459677866364	39.0	36.0	41.0	31.0	41.0
29	37.033451280176706	39.0	36.0	41.0	31.0	41.0
30	36.9133887720933	39.0	35.0	41.0	31.0	41.0
31	36.68231790001243	39.0	35.0	40.0	30.0	41.0
32	36.526518657688364	39.0	35.0	40.0	30.0	41.0
33	36.36359424015581	39.0	35.0	40.0	30.0	41.0
34	36.18158409844208	38.0	35.0	40.0	30.0	41.0
35	35.98385310491714	38.0	35.0	40.0	29.0	41.0
36	35.92590071380362	38.0	35.0	40.0	28.0	41.0
37	35.790743920098045	38.0	35.0	40.0	27.0	41.0
38	35.665405865992724	38.0	35.0	40.0	27.0	41.0
39	35.550246297209384	38.0	35.0	40.0	26.0	41.0
40	35.38006755104458	38.0	35.0	40.0	25.0	41.0
41	35.25851189896108	38.0	34.0	40.0	25.0	41.0
42	34.985695307328996	38.0	34.0	40.0	24.0	41.0
43	34.61431835069085	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	10.0
13	7.0
14	13.0
15	27.0
16	60.0
17	154.0
18	325.0
19	858.0
20	1760.0
21	3436.0
22	6354.0
23	10999.0
24	18193.0
25	27658.0
26	41185.0
27	58104.0
28	79709.0
29	106040.0
30	134535.0
31	166749.0
32	205181.0
33	255421.0
34	320615.0
35	398995.0
36	529118.0
37	773927.0
38	1054796.0
39	1326333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.04333396249588	20.692932572493472	13.912305368688646	23.351428096322007
2	18.193859107924805	23.51526999224355	35.50005561023997	22.790815289591684
3	19.221724728949894	24.40295071193787	32.92700422384227	23.44832033526997
4	13.178884194120673	16.165697502756057	36.77342142091952	33.881996882203744
5	12.531178868253726	38.80391974301185	34.926110837185895	13.738790551548519
6	32.07873975240945	37.96262919418045	13.985703639807948	15.972927413602156
7	26.271472888830598	33.37898686475263	21.444612744417874	18.904927501998888
8	27.477309391826854	34.44210249456306	19.068334659888134	19.012253453721957
9	24.98502870901281	14.450963904788022	21.50723313515317	39.056774251046
10	16.17651161130942	28.053011231094782	33.24789523393073	22.522581923665076
11	33.478107136116115	22.736726632740194	23.30909548042719	20.476070750716502
12	21.35496613934151	27.351760670916715	30.986478560350516	20.306794629391263
13	31.547852158637358	20.843768555615494	26.056694186791717	21.551685098955435
14	20.6023440350138	22.472876875305904	28.46824039419146	28.45653869548883
15	22.88316089328522	29.743979149963973	25.192942172958354	22.179917783792458
16	21.769543195389748	28.266069095089165	26.486106678192055	23.478281031329036
17	21.733405596455146	27.01255632121779	27.384510936016344	23.869527146310723
18	22.700371664789444	25.721402479383453	29.725339756829282	21.85288609899782
19	22.711765424052533	24.984920024504735	31.027072224116147	21.27624232732658
20	23.39698501928969	24.93269711837518	30.374222498200364	21.296095364134764
21	22.33292745707596	25.65852849146265	30.926720194994502	21.08182385646689
22	21.41139151311659	26.062671834735784	30.51205256852286	22.01388408362476
23	22.184120251437985	26.019053118828754	30.840533380091824	20.95629324964143
24	21.92831314760117	26.110982098574674	29.44372008232489	22.516984671499262
25	22.1101061014396	26.057581776940985	30.3385739795521	21.49373814206732
26	21.542954110140155	27.000764052091757	30.3226154709499	21.133666366818186
27	20.79759575376873	26.96837606868571	30.082875560223354	22.151152617322207
28	21.24533969886421	26.757890404715752	30.049599986668035	21.947169909752006
29	21.425592955504925	26.684166080072224	29.604881093713942	22.28535987070891
30	21.371848466262335	27.037970382022426	30.75617608774173	20.834005063973514
31	22.053191647378185	26.762346469546785	30.507723302284585	20.67673858079045
32	21.478377398259525	26.723364959317575	29.78565965881035	22.01259798361255
33	21.202862170291958	26.570771909981694	30.41092163376002	21.81544428596633
34	20.744539599743938	26.929104733101646	30.8083808797866	21.517974787367816
35	20.38660528648693	28.563810304957858	29.930391195395543	21.119193213159665
36	22.148489846874398	26.96352149399174	29.647720903979774	21.24026775515409
37	21.233764798754333	27.349532638501195	29.755825761344035	21.660876801400438
38	21.003389869806828	26.380247967327985	30.75652025535063	21.859841907514554
39	20.613248713990558	26.17747890343128	31.43851554351492	21.77075683906324
40	19.826753271313123	26.548527813995882	31.248879191010488	22.375839723680507
41	20.732729216533233	25.6210504502618	31.48137346786543	22.164846865339534
42	19.82372821917173	27.1233782912839	30.83172993493783	22.221163554606537
43	19.853670801146116	26.762237785038707	30.500676923344454	22.883414490470724
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3354.0
1	2412.5
2	1471.0
3	3881.5
4	6292.0
5	6292.0
6	8969.0
7	11646.0
8	11966.5
9	12287.0
10	18541.0
11	24795.0
12	24795.0
13	44819.0
14	64843.0
15	107876.0
16	150909.0
17	136512.0
18	122115.0
19	122115.0
20	124587.5
21	127060.0
22	102018.0
23	76976.0
24	88043.0
25	99110.0
26	99110.0
27	111747.5
28	124385.0
29	134877.0
30	145369.0
31	158994.0
32	172619.0
33	172619.0
34	191454.5
35	210290.0
36	228700.0
37	247110.0
38	262656.0
39	278202.0
40	278202.0
41	288300.0
42	298398.0
43	315019.0
44	331640.0
45	349526.5
46	367413.0
47	367413.0
48	447457.5
49	527502.0
50	497911.0
51	468320.0
52	429757.5
53	391195.0
54	391195.0
55	355746.5
56	320298.0
57	281417.0
58	242536.0
59	219954.5
60	197373.0
61	197373.0
62	175420.5
63	153468.0
64	133087.5
65	112707.0
66	95728.0
67	78749.0
68	78749.0
69	67537.0
70	56325.0
71	47554.0
72	38783.0
73	31548.5
74	24314.0
75	24314.0
76	19453.5
77	14593.0
78	11474.5
79	8356.0
80	6505.0
81	4654.0
82	4654.0
83	3484.5
84	2315.0
85	1821.0
86	1327.0
87	1017.0
88	707.0
89	707.0
90	589.0
91	471.0
92	324.5
93	178.0
94	124.0
95	70.0
96	70.0
97	50.0
98	30.0
99	20.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5520566.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.49723695853101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.10224920192333	31.491408434942514
2	13.171639717012537	11.195165883725606
3	4.970024076882164	6.336368726545971
4	2.3961852711924925	4.07325013065637
5	1.3242124449229529	2.813768502766322
6	0.8205774045977292	2.092336344360364
7	0.5469881479703217	1.6271839456461947
8	0.3855180671237645	1.3106762120282789
9	0.2908524478062564	1.1124382854952126
>10	1.7247361399467531	13.716260786997248
>50	0.15517659800572617	4.578208461549235
>100	0.09941519993429035	7.881126731151452
>500	0.007643045724978483	2.2170613933596135
>1k	0.004099060248614651	3.643820178632458
>5k	2.9888980979432653E-4	0.8806108952730685
>10k+	3.8428689830699125E-4	5.030315086870172
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	80632	1.4605748758370067	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	57530	1.0421032915827835	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	56417	1.021942315335058	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25427	0.46058683113289467	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	12607	0.228364265548134	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12355	0.22379951620902638	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11710	0.2121159315910724	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	10324	0.18700981022598046	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10216	0.18505348908064861	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9119	0.16518233818778727	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7707	0.1396052506210414	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7190	0.13024026884199916	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6864	0.12433507723664566	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6413	0.11616562504641734	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5947	0.10772446158600404	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	5.434225403699548E-5	0.0	0.0	0.0	0.0
7	5.434225403699548E-5	0.0	0.0	0.0	0.0
8	9.057042339499247E-5	0.0	0.0	0.0	0.0
9	9.057042339499247E-5	0.0	0.0	3.6228169357996986E-5	0.0
10	1.0868450807399097E-4	0.0	0.0	9.057042339499247E-5	0.0
11	1.0868450807399097E-4	0.0	0.0	1.2679859275298945E-4	0.0
12	1.0868450807399097E-4	0.0	0.0	1.9925493146898343E-4	0.0
13	1.0868450807399097E-4	0.0	0.0	2.3548310082698043E-4	0.0
14	1.4491267743198794E-4	0.0	0.0	3.441676089009714E-4	0.0
15	1.4491267743198794E-4	0.0	0.0	4.7096620165396085E-4	0.0
16	1.4491267743198794E-4	0.0	0.0	8.151338105549322E-4	0.0
17	1.4491267743198794E-4	0.0	0.0	0.0011955295888139007	0.0
18	1.4491267743198794E-4	0.0	0.0	0.0014128986049618826	0.0
19	1.4491267743198794E-4	0.0	0.0	0.0018295225525788478	0.0
20	1.6302676211098644E-4	0.0	0.0	0.0029888239720347516	0.0
21	1.6302676211098644E-4	0.0	0.0	0.0059595338593905044	0.0
22	1.6302676211098644E-4	0.0	0.0	0.009745377557301189	0.0
23	1.6302676211098644E-4	0.0	0.0	0.012353805751076972	0.0
24	1.9925493146898343E-4	0.0	0.0	0.015849824094123683	0.0
25	1.9925493146898343E-4	0.0	0.0	0.01825899735643048	0.0
26	1.9925493146898343E-4	0.0	0.0	0.024109846707746997	0.0
27	1.9925493146898343E-4	0.0	0.0	0.05955911042454705	0.0
28	1.9925493146898343E-4	0.0	0.0	0.14860795070650365	0.0
29	1.9925493146898343E-4	0.0	0.0	0.288358114004977	0.0
30	1.9925493146898343E-4	0.0	0.0	0.4666731635850382	0.0
31	1.9925493146898343E-4	0.0	0.0	0.7322256449791561	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20390	0.0	19.924475	1
ATTGGAC	1875	0.0	17.759998	3
GTATTGG	1935	0.0	17.209303	1
TTGGACC	2345	0.0	17.040512	4
GCGTAAG	85	5.3687405E-4	15.235294	1
TGGACCC	2615	0.0	14.644359	5
GGACCCT	2515	0.0	14.638171	6
TATTGGA	2310	0.0	14.575758	2
GTATTAG	1235	0.0	14.380568	1
TATACCG	435	0.0	14.034483	5
TATACAC	2210	0.0	13.979638	37
CTAATAC	1175	0.0	13.855319	3
ACGTTCG	510	0.0	13.784313	22
TAGGTCG	310	0.0	13.725806	21
CTAGTAC	805	0.0	13.559007	3
GACCCTC	2590	0.0	13.5	7
TAGTACT	855	0.0	13.415205	4
CGTTCGC	535	0.0	13.140186	23
CTGTGCG	565	0.0	12.769912	9
TATACTG	855	0.0	12.766082	5
>>END_MODULE