FastQCFastQC Report
Wed 25 May 2016
SRR2088546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3679261
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT983392.6727921721236956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT678311.8436039193740266No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT670891.8234368260365328No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295050.8019273435616555No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG157280.4274771482642846No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA143480.3899696161810755No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA127130.34553134447379513No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG126790.3446072458572523No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG125510.3411282863596793No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA116450.31650377616592024No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA105860.2877208221977185No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT87430.23762924130688198No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC87270.23719437136968538No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA71140.1933540458260504No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA65250.17734539626299956No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT64170.1744100241869223No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62470.16978953110420814No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT55550.15098140632045404No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA52140.14171324078395092No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG49880.13557070292104853No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49510.13456506619128136No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC49320.13404865814086034No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA49270.1339127612854864No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG48690.13233635776314864No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA48640.13220046090777468No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47960.13035226367468902No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA46890.12744407096968657No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT46180.12551433562337655No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA45780.12442716078038497No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA45140.12268768103159847No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT44920.1220897348679531No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC44630.12130153310678422No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT43090.11711590996126668No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG41330.11233234065210378No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT39650.10776620631153919No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37930.10309135448667545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG16750.020.653731
ACGTTTA1850.020.026
ATTGGAC18350.019.8610343
GGTATCA247550.019.2809541
CGAATTA2050.018.0487815
TTGGACC24800.017.9778214
TATTGGA20250.017.449382
TGGACCC25500.017.3392165
GGACCCT24900.017.3112456
TTCGTTA4300.017.20930330
CAGTCGA650.001580827517.07692326
TCACGTT2350.016.53191424
TATACAC22600.016.28982437
TATACCG1951.8189894E-1216.1282065
CGTTCGC5150.015.80582623
TAGTACT5350.015.5607474
ACTCTGT20250.015.43950637
ACGTTCG5050.015.38613822
TATACTG3500.015.3285715
CGACGAA5900.015.05084710
Produced by FastQC (version 0.11.5)