##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088546_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3679261
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.690602813989006	31.0	31.0	34.0	30.0	34.0
2	31.8151794613103	31.0	31.0	34.0	30.0	34.0
3	31.915774118770045	31.0	31.0	34.0	30.0	34.0
4	35.57515191229978	37.0	35.0	37.0	33.0	37.0
5	35.52798265738691	37.0	35.0	37.0	33.0	37.0
6	35.61156547469723	37.0	35.0	37.0	33.0	37.0
7	35.4364648226913	37.0	35.0	37.0	33.0	37.0
8	35.51697555568904	37.0	35.0	37.0	33.0	37.0
9	37.07745604348265	39.0	37.0	39.0	33.0	39.0
10	36.96512533359281	39.0	37.0	39.0	33.0	39.0
11	37.02842309909517	39.0	37.0	39.0	33.0	39.0
12	36.877014976648844	39.0	37.0	39.0	32.0	39.0
13	36.94276133169134	39.0	37.0	39.0	33.0	39.0
14	37.85436368879511	40.0	37.0	41.0	32.0	41.0
15	37.8760049912197	40.0	37.0	41.0	32.0	41.0
16	37.7620516728767	40.0	37.0	41.0	32.0	41.0
17	37.88251173265501	40.0	37.0	41.0	33.0	41.0
18	37.89371099250638	40.0	37.0	41.0	33.0	41.0
19	37.98258590515867	40.0	37.0	41.0	33.0	41.0
20	37.90807447473827	40.0	37.0	41.0	32.0	41.0
21	37.789639821692454	40.0	37.0	41.0	32.0	41.0
22	37.680444252256095	40.0	37.0	41.0	32.0	41.0
23	37.49220645124116	39.0	36.0	41.0	32.0	41.0
24	37.60428221863032	40.0	36.0	41.0	32.0	41.0
25	37.53027034505027	40.0	36.0	41.0	32.0	41.0
26	37.29255168361255	39.0	36.0	41.0	31.0	41.0
27	37.237929573357256	39.0	36.0	41.0	31.0	41.0
28	37.05655836865066	39.0	35.0	41.0	31.0	41.0
29	36.80994661699727	39.0	35.0	41.0	30.0	41.0
30	36.60793050560969	39.0	35.0	40.0	30.0	41.0
31	36.26347763858014	38.0	35.0	40.0	30.0	41.0
32	36.075362688322464	38.0	35.0	40.0	30.0	41.0
33	35.8585509427029	38.0	35.0	40.0	30.0	41.0
34	35.6380566640964	38.0	35.0	40.0	29.0	41.0
35	35.38458565456487	38.0	34.0	40.0	27.0	41.0
36	35.225419996026375	38.0	34.0	40.0	25.0	41.0
37	35.012249470749694	38.0	34.0	40.0	25.0	41.0
38	34.8187576255123	38.0	34.0	40.0	24.0	41.0
39	34.62203442484782	38.0	33.0	40.0	23.0	41.0
40	34.34794813414977	38.0	33.0	40.0	21.0	41.0
41	34.12285537775113	38.0	33.0	40.0	20.0	41.0
42	33.72002665752715	37.0	33.0	40.0	18.0	41.0
43	33.271863833525266	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	4.0
13	10.0
14	8.0
15	23.0
16	55.0
17	117.0
18	256.0
19	650.0
20	1319.0
21	2804.0
22	5194.0
23	8923.0
24	14771.0
25	22783.0
26	33090.0
27	47096.0
28	64141.0
29	83311.0
30	103308.0
31	124185.0
32	149635.0
33	184033.0
34	226547.0
35	277164.0
36	367622.0
37	560924.0
38	645783.0
39	755499.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.7883208068142	20.901208150223646	15.643467533289973	20.66700350967219
2	18.413344418892816	25.21177486457199	35.365471490062816	21.009409226472382
3	20.098791577982645	24.55368075273812	34.592028127387536	20.7554995418917
4	12.730817411431264	15.90699871523113	35.8870708003591	35.475113072978516
5	11.705149485181943	40.00284296221442	34.09187334086926	14.200134211734367
6	31.419597576795994	37.73195215017363	12.496205080313683	18.35224519271669
7	24.203746350150208	35.20041116952562	20.578290042484074	20.0175524378401
8	28.76224328744278	33.4038003827399	18.15111784676325	19.68283848305407
9	23.99487288344045	14.339836179058786	22.71249579738975	38.95279514011102
10	16.871975105870447	28.809236420031088	31.474826058819964	22.843962415278504
11	32.09016701995319	23.02793957808375	23.60911606977597	21.27277733218709
12	21.92951247546722	29.058416894044754	30.43684587747377	18.57522475301426
13	33.510859925403494	20.989187774392736	26.430008634886192	19.069943665317574
14	19.916662612410484	24.036593218040252	30.020512271350142	26.02623189819912
15	21.855530227401644	30.788247966099714	26.93054393259951	20.425677873899133
16	19.97091263707576	28.661054488931338	27.891443417577605	23.476589456415297
17	20.77718868000938	26.937121340399607	28.122141919260418	24.163548060330594
18	22.621689518628877	24.73752201868799	31.830957358012927	20.809831104670202
19	21.90401822539907	24.03917525829236	34.196214946425386	19.86059156988319
20	22.986463857823622	23.783498914591817	33.324137646119695	19.905899581464865
21	21.18384643003038	24.997383985534054	34.55479238901508	19.26397719542049
22	19.361252164497163	25.75234537587847	33.80687045577903	21.07953200384534
23	20.828666408825033	25.829534789730875	34.60382397443399	18.7379748270101
24	20.44206703465723	26.055803053928493	32.11337820285106	21.388751708563213
25	20.319297815512407	26.215590576477176	33.53814801396259	19.926963594047827
26	19.733826983190376	27.70866214709965	33.46250782426145	19.09500304544853
27	18.505754280547098	27.38052560011372	33.15179325413446	20.961926865204724
28	19.210515372516383	27.144581479813475	33.00453542165125	20.640367726018894
29	19.460837380115194	26.943454133860033	31.920377488848985	21.67533099717579
30	19.34296044776383	27.45488835937434	34.483500898685904	18.718650294175923
31	20.608758117458915	27.160562950005453	33.87199222887422	18.358686703661416
32	19.812049213143617	27.068071550237942	32.509734971234714	20.610144265383727
33	19.262237715671706	26.95234178820149	33.443754058219845	20.341666437906962
34	18.178650549662013	27.931043761233575	34.10220150187769	19.78810418722673
35	17.59410381595652	30.777403397040874	32.428441472350016	19.20005131465259
36	20.72138943119284	27.752747086982954	32.24742686099192	19.27843662083228
37	19.5045961675456	29.11394434915055	31.84373166241808	19.53772782088577
38	19.133815187343327	26.932854179140865	33.59636622680478	20.336964406711022
39	18.51681628457454	26.71607151544835	34.46640507427986	20.300707125697254
40	17.08011472956118	27.355520578724914	34.104702004016566	21.45966268769734
41	18.938830379252792	26.193439389051225	34.241387061151684	20.626343170544303
42	17.732963222777617	28.520564319845754	33.13178923702341	20.614683220353218
43	17.79096400065122	28.530403252174825	32.25517298174824	21.42345976542572
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1115.0
1	1125.5
2	1136.0
3	3409.5
4	5683.0
5	5683.0
6	8884.0
7	12085.0
8	13014.0
9	13943.0
10	21063.0
11	28183.0
12	28183.0
13	51594.0
14	75005.0
15	127621.5
16	180238.0
17	159347.0
18	138456.0
19	138456.0
20	135983.5
21	133511.0
22	98355.5
23	63200.0
24	68149.5
25	73099.0
26	73099.0
27	77779.5
28	82460.0
29	84622.0
30	86784.0
31	90414.0
32	94044.0
33	94044.0
34	102245.5
35	110447.0
36	122325.0
37	134203.0
38	140377.5
39	146552.0
40	146552.0
41	152777.5
42	159003.0
43	171610.0
44	184217.0
45	206337.5
46	228458.0
47	228458.0
48	332246.0
49	436034.0
50	397162.5
51	358291.0
52	315598.0
53	272905.0
54	272905.0
55	236903.5
56	200902.0
57	165205.0
58	129508.0
59	112870.5
60	96233.0
61	96233.0
62	84336.0
63	72439.0
64	62772.0
65	53105.0
66	45274.5
67	37444.0
68	37444.0
69	31866.5
70	26289.0
71	22041.5
72	17794.0
73	14705.5
74	11617.0
75	11617.0
76	9184.5
77	6752.0
78	5261.5
79	3771.0
80	2967.5
81	2164.0
82	2164.0
83	1637.5
84	1111.0
85	824.5
86	538.0
87	407.0
88	276.0
89	276.0
90	223.5
91	171.0
92	114.5
93	58.0
94	41.5
95	25.0
96	25.0
97	16.5
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3679261.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.179799686090476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0642845629149	28.280548633149603
2	12.357911634170819	9.189293581937559
3	4.431173690335754	4.942504505428729
4	2.095147971899957	3.115887276318365
5	1.1769377706440316	2.187915527776949
6	0.6934265989029704	1.5468877226531668
7	0.5060250260213784	1.3169736372536498
8	0.36471081398168875	1.0847900005752154
9	0.2577341762575251	0.8624254540962887
>10	1.7160069886878175	12.382939516155126
>50	0.1835309323242606	4.771039887580199
>100	0.13151332949356478	9.264091757840736
>500	0.012193944641720994	3.09770800229726
>1k	0.008006867264669112	6.8260897114892956
>5k	5.876599827280083E-4	1.4896156817907351
>10k+	8.080324762510113E-4	9.641289103657265
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	98339	2.6727921721236956	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	67831	1.8436039193740266	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	67089	1.8234368260365328	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29505	0.8019273435616555	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	15728	0.4274771482642846	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	14348	0.3899696161810755	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	12713	0.34553134447379513	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	12679	0.3446072458572523	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12551	0.3411282863596793	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11645	0.31650377616592024	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	10586	0.2877208221977185	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8743	0.23762924130688198	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	8727	0.23719437136968538	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7114	0.1933540458260504	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6525	0.17734539626299956	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	6417	0.1744100241869223	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6247	0.16978953110420814	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	5555	0.15098140632045404	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5214	0.14171324078395092	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	4988	0.13557070292104853	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4951	0.13456506619128136	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4932	0.13404865814086034	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4927	0.1339127612854864	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	4869	0.13233635776314864	No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA	4864	0.13220046090777468	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4796	0.13035226367468902	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4689	0.12744407096968657	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	4618	0.12551433562337655	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	4578	0.12442716078038497	No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA	4514	0.12268768103159847	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	4492	0.1220897348679531	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4463	0.12130153310678422	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4309	0.11711590996126668	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4133	0.11233234065210378	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	3965	0.10776620631153919	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3793	0.10309135448667545	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7179371074789204E-5
2	0.0	0.0	0.0	0.0	2.7179371074789204E-5
3	0.0	0.0	0.0	0.0	2.7179371074789204E-5
4	0.0	0.0	0.0	0.0	2.7179371074789204E-5
5	0.0	0.0	0.0	0.0	2.7179371074789204E-5
6	2.7179371074789204E-5	0.0	0.0	2.7179371074789204E-5	5.435874214957841E-5
7	2.7179371074789204E-5	0.0	0.0	2.7179371074789204E-5	5.435874214957841E-5
8	5.435874214957841E-5	0.0	0.0	8.153811322436762E-5	5.435874214957841E-5
9	1.35896855373946E-4	0.0	0.0	1.6307622644873523E-4	5.435874214957841E-5
10	1.35896855373946E-4	0.0	0.0	1.9025559752352443E-4	5.435874214957841E-5
11	1.6307622644873523E-4	0.0	0.0	2.71793710747892E-4	5.435874214957841E-5
12	1.6307622644873523E-4	0.0	0.0	4.892286793462057E-4	5.435874214957841E-5
13	1.6307622644873523E-4	0.0	0.0	5.979461636453625E-4	5.435874214957841E-5
14	1.6307622644873523E-4	0.0	0.0	8.697398743932545E-4	5.435874214957841E-5
15	1.9025559752352443E-4	0.0	0.0	0.0012774304405150925	5.435874214957841E-5
16	1.9025559752352443E-4	0.0	0.0	0.0022015290570579255	5.435874214957841E-5
17	1.9025559752352443E-4	0.0	0.0	0.003370242013273861	5.435874214957841E-5
18	1.9025559752352443E-4	0.0	0.0	0.0041856231455175376	5.435874214957841E-5
19	2.1743496859831364E-4	0.0	0.0	0.006359972831500674	5.435874214957841E-5
20	2.1743496859831364E-4	0.0	0.0	0.009974829184447637	5.435874214957841E-5
21	2.1743496859831364E-4	0.0	0.0	0.018318896104407924	5.435874214957841E-5
22	2.1743496859831364E-4	0.0	0.0	0.030332178119464752	5.435874214957841E-5
23	2.1743496859831364E-4	0.0	0.0	0.036746509693115006	5.435874214957841E-5
24	2.1743496859831364E-4	0.0	0.0	0.0468300563618618	5.435874214957841E-5
25	2.1743496859831364E-4	0.0	0.0	0.054086948438830516	5.435874214957841E-5
26	2.1743496859831364E-4	0.0	0.0	0.06544792554809241	5.435874214957841E-5
27	2.1743496859831364E-4	0.0	0.0	0.11964359147122207	5.435874214957841E-5
28	2.1743496859831364E-4	0.0	0.0	0.260840424204752	5.435874214957841E-5
29	2.1743496859831364E-4	0.0	0.0	0.502465033059628	5.435874214957841E-5
30	2.1743496859831364E-4	0.0	0.0	0.8181806074643794	5.435874214957841E-5
31	2.1743496859831364E-4	0.0	0.0	1.182628794206228	5.435874214957841E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	1675	0.0	20.65373	1
ACGTTTA	185	0.0	20.0	26
ATTGGAC	1835	0.0	19.861034	3
GGTATCA	24755	0.0	19.280954	1
CGAATTA	205	0.0	18.04878	15
TTGGACC	2480	0.0	17.977821	4
TATTGGA	2025	0.0	17.44938	2
TGGACCC	2550	0.0	17.339216	5
GGACCCT	2490	0.0	17.311245	6
TTCGTTA	430	0.0	17.209303	30
CAGTCGA	65	0.0015808275	17.076923	26
TCACGTT	235	0.0	16.531914	24
TATACAC	2260	0.0	16.289824	37
TATACCG	195	1.8189894E-12	16.128206	5
CGTTCGC	515	0.0	15.805826	23
TAGTACT	535	0.0	15.560747	4
ACTCTGT	2025	0.0	15.439506	37
ACGTTCG	505	0.0	15.386138	22
TATACTG	350	0.0	15.328571	5
CGACGAA	590	0.0	15.050847	10
>>END_MODULE