FastQCFastQC Report
Wed 25 May 2016
SRR2088545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043565
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT184711.7699903695505312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT129391.2398844346063733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT127331.2201444088293494No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56810.5443839147537528No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG32320.3097075888900068No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA27740.2658195704148759No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA27640.26486131673637964No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG26980.258536842458304No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG25770.24694197294849862No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25730.24655867147710012No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA18750.17967256471805781No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17340.16616118785125988No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC15590.14939174847757447No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA14430.1382760058070173No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA12350.11834432929429407No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12200.11690694877654961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG4350.020.8390791
GGTATCA47600.020.7542021
TAGACTG1001.2894816E-820.355
GTACTAG1101.7535058E-920.1818181
CTCTAAT1403.6379788E-1219.821431
ATTGGAC5000.018.8699993
TCGTTAT609.236037E-418.513
TGGACCC5800.018.55
CAATACG500.007034458718.54
CTAGGCA1351.1514203E-917.8148144
TTGGACC6550.017.7938924
CGGAACT852.7227261E-517.4117644
GGACCCT6200.017.3064526
CTAGGAC752.0670365E-417.2666663
TAGGCAT1005.8767473E-616.6500025
AGTACCG1005.8767473E-616.6500025
GTTCTAT904.445326E-516.4444451
CTCTATA803.3821928E-416.18752
TTCGTTA1151.2418495E-616.08695630
GACCCTC6700.016.0149257
Produced by FastQC (version 0.11.5)