##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088545_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1043565
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.699180213978046	31.0	31.0	34.0	30.0	34.0
2	31.84276781992497	31.0	31.0	34.0	30.0	34.0
3	31.961890251206203	33.0	31.0	34.0	30.0	34.0
4	35.63223277898358	37.0	35.0	37.0	33.0	37.0
5	35.55493716251503	37.0	35.0	37.0	33.0	37.0
6	35.61359761969786	37.0	35.0	37.0	33.0	37.0
7	35.46356575776305	37.0	35.0	37.0	33.0	37.0
8	35.514978942375414	37.0	35.0	37.0	33.0	37.0
9	37.09857843066795	39.0	37.0	39.0	33.0	39.0
10	36.962354045986594	39.0	37.0	39.0	33.0	39.0
11	37.029504630760904	39.0	37.0	39.0	33.0	39.0
12	36.87961459037051	39.0	37.0	39.0	32.0	39.0
13	36.93744615812144	39.0	37.0	39.0	33.0	39.0
14	37.938501195421466	40.0	37.0	41.0	33.0	41.0
15	37.948861834193366	40.0	37.0	41.0	33.0	41.0
16	37.83703937943492	40.0	37.0	41.0	32.0	41.0
17	37.88589115196466	40.0	37.0	41.0	33.0	41.0
18	37.86767858255116	40.0	37.0	41.0	33.0	41.0
19	37.9310354410123	40.0	37.0	41.0	32.0	41.0
20	37.8427841102375	40.0	37.0	41.0	32.0	41.0
21	37.70147810629908	40.0	37.0	41.0	32.0	41.0
22	37.594536995778896	39.0	37.0	41.0	32.0	41.0
23	37.40720702591597	39.0	36.0	41.0	32.0	41.0
24	37.55563572944666	39.0	36.0	41.0	32.0	41.0
25	37.50355464202038	39.0	36.0	41.0	32.0	41.0
26	37.2905061016803	39.0	36.0	41.0	31.0	41.0
27	37.245756613148195	39.0	36.0	41.0	31.0	41.0
28	37.09421262690872	39.0	36.0	41.0	31.0	41.0
29	36.87924757921164	39.0	35.0	41.0	30.0	41.0
30	36.73259451974721	39.0	35.0	41.0	30.0	41.0
31	36.45898530517984	39.0	35.0	40.0	30.0	41.0
32	36.29869533761673	38.0	35.0	40.0	30.0	41.0
33	36.11294265330861	38.0	35.0	40.0	30.0	41.0
34	35.91645082002559	38.0	35.0	40.0	29.0	41.0
35	35.70307934819585	38.0	35.0	40.0	27.0	41.0
36	35.62254483429398	38.0	35.0	40.0	27.0	41.0
37	35.47221016419677	38.0	35.0	40.0	26.0	41.0
38	35.33697469731162	38.0	34.0	40.0	25.0	41.0
39	35.20403712274751	38.0	34.0	40.0	25.0	41.0
40	35.0033510131137	38.0	34.0	40.0	24.0	41.0
41	34.8590849635624	38.0	34.0	40.0	23.0	41.0
42	34.5487487602593	38.0	33.0	40.0	22.0	41.0
43	34.14985362675061	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	0.0
13	2.0
14	3.0
15	10.0
16	20.0
17	23.0
18	77.0
19	181.0
20	323.0
21	697.0
22	1301.0
23	2326.0
24	3789.0
25	5862.0
26	8684.0
27	12184.0
28	16576.0
29	21772.0
30	27484.0
31	33613.0
32	41065.0
33	50190.0
34	62830.0
35	77181.0
36	101942.0
37	149737.0
38	195500.0
39	230190.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.64350088398902	21.153066651334605	14.695490937315835	22.50794152736054
2	18.296416610369263	23.988155984533783	35.66773511951819	22.04769228557876
3	19.41048233698907	24.746517945695764	33.331895952815586	22.511103764499577
4	13.229266983848634	16.278909315663135	36.47707617637617	34.01474752411206
5	12.207193610364472	39.12779750183266	34.878038263069385	13.786970624733485
6	31.734199594658698	38.13552581774973	13.525175719768296	16.60509886782328
7	25.360854378979745	33.88519162677936	21.53157685434065	19.222377139900246
8	27.46019653782946	34.453436058127664	18.93288870362651	19.15347870041636
9	24.478398566452498	14.542266174124277	21.987801430672743	38.991533828750484
10	16.356432038253487	28.201980710353453	32.94859448141707	22.492992769975995
11	32.99123677011015	22.882906191756145	23.500979814386262	20.624877223747443
12	21.20778293637675	28.100405820432844	30.891990436628287	19.79982080656212
13	31.878033471800986	21.044783985664527	26.372482787368302	20.704699755166185
14	20.281534930742215	22.9590873591966	28.887707042685413	27.871670667375774
15	22.334210135449155	30.077762286009975	25.936285712916778	21.651741865624086
16	21.125277294658215	28.406088743873166	27.01096721335039	23.457666748118232
17	21.343088355780427	26.94724334373039	27.643127165054405	24.066541135434782
18	22.671515430279857	25.530561105441446	30.303718503399406	21.494204960879294
19	22.617565748180514	24.702725752588485	31.74972330425033	20.929985194980667
20	23.323127931657346	24.629610996919215	31.27519608265896	20.772064988764473
21	22.005720774460624	25.46559150603939	31.99484459520969	20.533843124290293
22	20.71936103644718	25.903609262480053	31.55404790310139	21.822981797971376
23	21.663432560501743	25.93935212468797	32.27120495608802	20.126010358722265
24	21.523048396602032	26.10436340812503	30.233670159501326	22.13891803577161
25	21.4658406519958	26.22232443594793	31.290432316147054	21.021402595909215
26	21.055133125392285	27.23922323956821	31.150527279086592	20.55511635595291
27	20.011786520245504	27.1644794526455	30.97727501401446	21.846459013094535
28	20.63053092045057	26.68420270898315	30.988774058156416	21.696492312409866
29	20.841730031191158	26.676919981026575	30.243444347021985	22.237905640760278
30	20.856103836368604	26.99266456809111	31.792748894414817	20.358482701125467
31	21.61686143172682	26.69493515018231	31.632528879370238	20.055674538720638
32	21.002716649178538	26.557425747318085	30.724775169730684	21.715082433772693
33	20.60216661156708	26.51363355421081	31.440686492935278	21.443513341286838
34	19.90685774245016	27.017866639835564	31.99800683234873	21.07726878536555
35	19.615356973451583	28.908213671405232	30.783899421693906	20.692529933449283
36	21.69265929769588	26.78280701250042	30.583624402888177	20.940909286915524
37	20.703741501487688	27.46795839262528	30.570879628964175	21.257420476922857
38	20.484205583744185	26.09113950736178	31.798977543325048	21.625677365568986
39	20.000670777574946	25.86393756019031	32.58407478211707	21.551316880117675
40	18.936146766133398	26.35715072851236	32.38581209603618	22.320890409318057
41	20.17909761251096	25.545797338929532	32.331287461729744	21.943817586829763
42	19.17245212325059	27.372899627718443	31.58720348037736	21.867444768653606
43	19.311398906632554	27.180961415915633	30.996056786112984	22.511582891338826
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	293.0
1	284.5
2	276.0
3	745.5
4	1215.0
5	1215.0
6	1766.5
7	2318.0
8	2546.5
9	2775.0
10	4112.0
11	5449.0
12	5449.0
13	10033.5
14	14618.0
15	24511.5
16	34405.0
17	30744.0
18	27083.0
19	27083.0
20	27195.5
21	27308.0
22	21194.0
23	15080.0
24	16731.5
25	18383.0
26	18383.0
27	20401.0
28	22419.0
29	24183.0
30	25947.0
31	27987.0
32	30027.0
33	30027.0
34	34003.5
35	37980.0
36	41715.5
37	45451.0
38	48283.0
39	51115.0
40	51115.0
41	53269.5
42	55424.0
43	58646.0
44	61868.0
45	66763.0
46	71658.0
47	71658.0
48	91627.0
49	111596.0
50	103039.5
51	94483.0
52	85004.0
53	75525.0
54	75525.0
55	67027.0
56	58529.0
57	49918.5
58	41308.0
59	36790.5
60	32273.0
61	32273.0
62	28467.0
63	24661.0
64	21283.0
65	17905.0
66	15142.5
67	12380.0
68	12380.0
69	10582.5
70	8785.0
71	7388.0
72	5991.0
73	4918.5
74	3846.0
75	3846.0
76	3094.0
77	2342.0
78	1828.0
79	1314.0
80	1026.0
81	738.0
82	738.0
83	558.0
84	378.0
85	291.5
86	205.0
87	148.0
88	91.0
89	91.0
90	79.5
91	68.0
92	49.5
93	31.0
94	24.0
95	17.0
96	17.0
97	11.0
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1043565.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.111394108155906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.80282738665609	46.18490647483765
2	9.169388515847972	10.106755684553928
3	2.610116504858224	4.315414781023321
4	1.2416228216670722	2.737102586342984
5	0.6989080184971734	1.9258897626374023
6	0.44237265141512555	1.4627864120885317
7	0.3214001042807766	1.23989654693942
8	0.2387605472470371	1.0526741293448354
9	0.178864524906004	0.8871725991656568
>10	1.1521004792176486	11.903200946608266
>50	0.08500147129565046	3.2133967557098595
>100	0.04921317503624018	4.96439609706823
>500	0.006108017918965714	2.447814517833144
>1k	0.0026177219652710197	2.766643056068061
>5k	1.7451479768473466E-4	0.5463845929751323
>10k+	5.23544393054204E-4	4.245565056803602
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18471	1.7699903695505312	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12939	1.2398844346063733	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12733	1.2201444088293494	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5681	0.5443839147537528	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3232	0.3097075888900068	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2774	0.2658195704148759	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2764	0.26486131673637964	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2698	0.258536842458304	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2577	0.24694197294849862	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2573	0.24655867147710012	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1875	0.17967256471805781	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1734	0.16616118785125988	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1559	0.14939174847757447	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1443	0.1382760058070173	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1235	0.11834432929429407	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1220	0.11690694877654961	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.582536784963083E-5
2	0.0	0.0	0.0	0.0	9.582536784963083E-5
3	0.0	0.0	0.0	0.0	9.582536784963083E-5
4	0.0	0.0	0.0	0.0	9.582536784963083E-5
5	0.0	0.0	0.0	0.0	9.582536784963083E-5
6	0.0	0.0	0.0	0.0	1.9165073569926165E-4
7	0.0	0.0	0.0	9.582536784963083E-5	1.9165073569926165E-4
8	0.0	0.0	0.0	9.582536784963083E-5	1.9165073569926165E-4
9	0.0	0.0	0.0	9.582536784963083E-5	1.9165073569926165E-4
10	0.0	0.0	0.0	2.874761035488925E-4	1.9165073569926165E-4
11	0.0	0.0	0.0	9.582536784963083E-4	1.9165073569926165E-4
12	0.0	0.0	0.0	0.00114990441419557	1.9165073569926165E-4
13	0.0	0.0	0.0	0.0012457297820452008	2.874761035488925E-4
14	0.0	0.0	0.0	0.0015332058855940932	2.874761035488925E-4
15	0.0	0.0	0.0	0.002203983460541509	2.874761035488925E-4
16	0.0	0.0	0.0	0.0027789356676392943	2.874761035488925E-4
17	0.0	0.0	0.0	0.004216316185383756	2.874761035488925E-4
18	0.0	0.0	0.0	0.005078744496030434	2.874761035488925E-4
19	0.0	0.0	0.0	0.007474378692271205	2.874761035488925E-4
20	0.0	0.0	0.0	0.013128075395399424	4.791268392481542E-4
21	0.0	0.0	0.0	0.02203983460541509	4.791268392481542E-4
22	0.0	0.0	0.0	0.03785102030060418	4.791268392481542E-4
23	0.0	0.0	0.0	0.04839181076406357	4.791268392481542E-4
24	0.0	0.0	0.0	0.061328235423763734	4.791268392481542E-4
25	0.0	0.0	0.0	0.06937756632313272	5.74952207097785E-4
26	0.0	0.0	0.0	0.07972670605089285	5.74952207097785E-4
27	0.0	0.0	0.0	0.1286934690220542	5.74952207097785E-4
28	0.0	0.0	0.0	0.26371141232218404	5.74952207097785E-4
29	9.582536784963083E-5	0.0	0.0	0.45392476750370125	5.74952207097785E-4
30	9.582536784963083E-5	0.0	0.0	0.7199359886542764	5.74952207097785E-4
31	9.582536784963083E-5	0.0	0.0	1.0765021824227528	5.74952207097785E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	435	0.0	20.839079	1
GGTATCA	4760	0.0	20.754202	1
TAGACTG	100	1.2894816E-8	20.35	5
GTACTAG	110	1.7535058E-9	20.181818	1
CTCTAAT	140	3.6379788E-12	19.82143	1
ATTGGAC	500	0.0	18.869999	3
TCGTTAT	60	9.236037E-4	18.5	13
TGGACCC	580	0.0	18.5	5
CAATACG	50	0.0070344587	18.5	4
CTAGGCA	135	1.1514203E-9	17.814814	4
TTGGACC	655	0.0	17.793892	4
CGGAACT	85	2.7227261E-5	17.411764	4
GGACCCT	620	0.0	17.306452	6
CTAGGAC	75	2.0670365E-4	17.266666	3
TAGGCAT	100	5.8767473E-6	16.650002	5
AGTACCG	100	5.8767473E-6	16.650002	5
GTTCTAT	90	4.445326E-5	16.444445	1
CTCTATA	80	3.3821928E-4	16.1875	2
TTCGTTA	115	1.2418495E-6	16.086956	30
GACCCTC	670	0.0	16.014925	7
>>END_MODULE