FastQCFastQC Report
Wed 25 May 2016
SRR2088544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650195
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT300851.8231178739482303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT210811.2774853880904984No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT210571.2760310145164662No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87230.528604195261772No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG49850.3020855111062632No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA41800.2533033974772678No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA40600.24603152960710703No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG40490.24536494171900897No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA35690.2162774702383658No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA33000.199976366429422No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG32970.19979456973266796No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.1674953566093704No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC25380.1538000054539009No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA20550.12453073727650368No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT20380.12350055599489758No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA20130.12198558352194741No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19690.11931923196955511No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT17900.10847202906323192No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16870.10223034247467723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71500.021.2944071
GTCGACG609.239142E-418.536
GTATTGG5450.017.6513771
ATTGGAC5850.017.3931623
TTGGACC7550.017.1523194
TAGTACT2700.017.129634
TAGCACA1708.54925E-1116.323534
CTGTCCG803.3836826E-416.18759
CGTCTAT700.002593135715.85714227
TATACTG1751.3278623E-1015.8571425
GTATACT1302.5907684E-715.6538464
GTCTTAC957.062654E-515.5789481
GACCCTC7550.015.4370877
GGACCCT7700.015.1363636
CGAACTA1252.9604507E-614.80000129
CGTTTAG908.2767074E-414.38888826
TGGACCC7750.014.3225795
CCCTCGC7850.014.1401279
CTAGTAC2650.013.9622633
TTATGGG800.006300154613.8752
Produced by FastQC (version 0.11.5)