##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088544_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1650195
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.704440384318218	31.0	31.0	34.0	30.0	34.0
2	31.8520526362036	31.0	31.0	34.0	30.0	34.0
3	31.978215301827966	33.0	31.0	34.0	30.0	34.0
4	35.64779980547753	37.0	35.0	37.0	33.0	37.0
5	35.56326979538782	37.0	35.0	37.0	33.0	37.0
6	35.61697617554289	37.0	35.0	37.0	33.0	37.0
7	35.46551710555419	37.0	35.0	37.0	33.0	37.0
8	35.510391802180955	37.0	35.0	37.0	33.0	37.0
9	37.105869306354705	39.0	37.0	39.0	33.0	39.0
10	36.96577495386909	39.0	37.0	39.0	33.0	39.0
11	37.03323304215562	39.0	37.0	39.0	33.0	39.0
12	36.88549898648341	39.0	37.0	39.0	32.0	39.0
13	36.94195292071543	39.0	37.0	39.0	33.0	39.0
14	37.934953747890404	40.0	37.0	41.0	33.0	41.0
15	37.945368880647436	40.0	37.0	41.0	33.0	41.0
16	37.84266707873918	40.0	37.0	41.0	32.0	41.0
17	37.89310111835268	40.0	37.0	41.0	33.0	41.0
18	37.87564196958541	40.0	37.0	41.0	33.0	41.0
19	37.94691354658086	40.0	37.0	41.0	33.0	41.0
20	37.84217683364693	40.0	37.0	41.0	32.0	41.0
21	37.712394595790194	40.0	37.0	41.0	32.0	41.0
22	37.59345592490585	39.0	37.0	41.0	32.0	41.0
23	37.4149873196804	39.0	36.0	41.0	32.0	41.0
24	37.55462839240211	39.0	36.0	41.0	32.0	41.0
25	37.50629592260309	39.0	36.0	41.0	32.0	41.0
26	37.29184672114508	39.0	36.0	41.0	31.0	41.0
27	37.23985225988444	39.0	36.0	41.0	31.0	41.0
28	37.07981844569884	39.0	36.0	41.0	31.0	41.0
29	36.8431979250937	39.0	35.0	41.0	30.0	41.0
30	36.66363611573178	39.0	35.0	40.0	30.0	41.0
31	36.4032820363654	38.0	35.0	40.0	30.0	41.0
32	36.2376028287566	38.0	35.0	40.0	30.0	41.0
33	36.05118728392705	38.0	35.0	40.0	30.0	41.0
34	35.84172355388303	38.0	35.0	40.0	29.0	41.0
35	35.630946645699446	38.0	35.0	40.0	27.0	41.0
36	35.54358363708531	38.0	35.0	40.0	27.0	41.0
37	35.373302549092685	38.0	34.0	40.0	26.0	41.0
38	35.22181257366554	38.0	34.0	40.0	25.0	41.0
39	35.058639736515985	38.0	34.0	40.0	24.0	41.0
40	34.86126245686116	38.0	34.0	40.0	24.0	41.0
41	34.70745942146231	38.0	34.0	40.0	23.0	41.0
42	34.392797214874605	38.0	33.0	40.0	21.0	41.0
43	33.978673429503786	38.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	0.0
14	5.0
15	10.0
16	21.0
17	50.0
18	113.0
19	270.0
20	529.0
21	1108.0
22	2144.0
23	3732.0
24	6221.0
25	9512.0
26	14000.0
27	19795.0
28	26858.0
29	35108.0
30	43672.0
31	53681.0
32	65830.0
33	80411.0
34	99911.0
35	123441.0
36	161215.0
37	236830.0
38	302967.0
39	362759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43716348673945	21.213189956338493	15.005862943470316	22.34378361345174
2	18.299837291956404	24.196837343465468	35.71038574229106	21.792939622287065
3	19.151918409642494	24.560006544681084	33.72207526989235	22.565999775784075
4	13.266068555534346	16.441087265444388	36.3384933295762	33.95435084944506
5	12.08887434515315	39.300931102081876	34.69268783386206	13.917506718902917
6	31.480764394510953	38.310260302570306	13.571305209384347	16.6376700935344
7	24.86712176439754	34.51525425783014	21.31057238689973	19.307051590872593
8	27.371734855577674	34.50640681858811	18.95297222449468	19.168886101339538
9	24.53031308421126	14.679416674999016	22.10890228124555	38.681367959544176
10	16.382185135696083	28.53014340729429	32.67480509879135	22.412866358218274
11	32.75824978260145	23.182593572274794	23.517826681089204	20.541329964034556
12	21.32214677659307	28.335317947272898	30.75272922291002	19.589806053224013
13	31.975312008580804	21.451767821378688	26.17581558543081	20.3971045846097
14	20.09913979862986	23.51437254385088	28.949669584503653	27.436818073015612
15	22.138838137311044	30.69897799956975	25.852581058602166	21.30960280451704
16	20.958310987489355	28.86149818657795	26.928211514396782	23.25197931153591
17	21.186890034208076	27.57025684843306	27.569347864949297	23.673505252409562
18	22.449225697569076	25.929541660228033	30.473368298898006	21.14786434330488
19	22.386202842694345	25.235138877526598	32.02463951230006	20.354018767478994
20	23.043458500359048	25.114122876387334	31.279818445698844	20.562600177554774
21	21.758761843297307	25.892758128584802	32.11590145407058	20.23257857404731
22	20.494244619575262	26.385548374586033	31.54742318332076	21.572783822517945
23	21.51582085753502	26.366883913719285	32.125173085605034	19.99212214314066
24	21.14762194770921	26.553770917982423	30.391802180954375	21.906804953353998
25	21.13756253048882	26.69351197888734	31.518578107435786	20.65034738318805
26	20.59580837416184	27.767082072118747	31.32666139456246	20.310448159156948
27	19.638042776762745	27.558864255436475	31.13001796757353	21.673075000227247
28	20.24251679346986	27.343980559873227	31.03633206984629	21.37717057681062
29	20.470247455603733	27.159820506061404	30.36489626983478	22.005035768500086
30	20.557449271146744	27.508203575941025	32.05318159369045	19.881165559221788
31	21.404379482424805	27.21538969636922	31.56784501225613	19.812385808949852
32	20.64422689439733	27.227145882759313	30.788421974372724	21.340205248470635
33	20.30366108247813	26.83949472638082	31.549725941479643	21.307118249661404
34	19.594047976148275	27.245386151333626	32.326603825608494	20.83396204690961
35	19.18773235890304	29.096743112177652	31.130199764270284	20.585324764649027
36	21.278879162765612	27.04722775187175	30.927557046288467	20.74633603907417
37	20.37450119531328	27.598071743036428	30.952463193743768	21.074963867906522
38	20.020785422328878	26.188904947597102	32.32951257275655	21.460797057317468
39	19.45285254166932	26.05692054575368	33.09324049582019	21.396986416756807
40	18.393523189683645	26.373973984892697	33.02476374004284	22.20773908538082
41	19.487393914052582	25.51195464778405	33.16668636130882	21.833965076854554
42	18.64773557064468	27.23290277815652	32.31157529867683	21.807786352521973
43	18.552837694939083	27.148246116368064	31.88907977542048	22.409836413272373
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	569.0
1	524.5
2	480.0
3	1164.5
4	1849.0
5	1849.0
6	2796.0
7	3743.0
8	4093.5
9	4444.0
10	6756.5
11	9069.0
12	9069.0
13	16194.5
14	23320.0
15	40222.5
16	57125.0
17	51529.5
18	45934.0
19	45934.0
20	46167.5
21	46401.0
22	36724.5
23	27048.0
24	29852.5
25	32657.0
26	32657.0
27	35692.5
28	38728.0
29	40913.0
30	43098.0
31	46904.0
32	50710.0
33	50710.0
34	55708.0
35	60706.0
36	66284.0
37	71862.0
38	76199.0
39	80536.0
40	80536.0
41	83549.5
42	86563.0
43	91581.0
44	96599.0
45	103771.5
46	110944.0
47	110944.0
48	142787.0
49	174630.0
50	160885.5
51	147141.0
52	130768.0
53	114395.0
54	114395.0
55	101496.0
56	88597.0
57	75909.5
58	63222.0
59	56379.0
60	49536.0
61	49536.0
62	43280.5
63	37025.0
64	32270.5
65	27516.0
66	23338.0
67	19160.0
68	19160.0
69	16275.5
70	13391.0
71	11269.0
72	9147.0
73	7568.0
74	5989.0
75	5989.0
76	4783.5
77	3578.0
78	2783.5
79	1989.0
80	1594.0
81	1199.0
82	1199.0
83	917.5
84	636.0
85	489.0
86	342.0
87	251.0
88	160.0
89	160.0
90	126.5
91	93.0
92	69.0
93	45.0
94	29.0
95	13.0
96	13.0
97	8.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1650195.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.45945907200565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.25112614653956	41.8113900049222
2	10.498195477981035	10.804629210581597
3	3.1815046349708873	4.911555226520419
4	1.446090396897218	2.9766011837421122
5	0.8305988126245339	2.1371082801754344
6	0.538072732346985	1.6613359048783156
7	0.36985736103040906	1.3322861812697047
8	0.2621726523484934	1.079301029866117
9	0.2112896891721552	0.9785567801062186
>10	1.2433494284785982	12.014724824812221
>50	0.09851332549063664	3.474904373847032
>100	0.059676553089334804	5.688023029370545
>500	0.0047174266816279705	1.765566026231773
>1k	0.004363619680505873	4.451481591436625
>5k	1.1793566704069926E-4	0.5293906382104961
>10k+	3.5380700112209773E-4	4.383145714029195
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30085	1.8231178739482303	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21081	1.2774853880904984	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21057	1.2760310145164662	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8723	0.528604195261772	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4985	0.3020855111062632	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4180	0.2533033974772678	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4060	0.24603152960710703	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4049	0.24536494171900897	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3569	0.2162774702383658	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3300	0.199976366429422	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3297	0.19979456973266796	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2764	0.1674953566093704	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2538	0.1538000054539009	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2055	0.12453073727650368	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2038	0.12350055599489758	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2013	0.12198558352194741	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1969	0.11931923196955511	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1790	0.10847202906323192	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1687	0.10223034247467723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	6.059889891800666E-5	0.0	0.0	0.0	0.0
6	6.059889891800666E-5	0.0	0.0	0.0	0.0
7	6.059889891800666E-5	0.0	0.0	0.0	0.0
8	6.059889891800666E-5	0.0	0.0	0.0	0.0
9	6.059889891800666E-5	0.0	0.0	1.2119779783601332E-4	6.059889891800666E-5
10	6.059889891800666E-5	0.0	0.0	2.4239559567202664E-4	6.059889891800666E-5
11	1.8179669675401998E-4	0.0	0.0	6.665878880980732E-4	6.059889891800666E-5
12	1.8179669675401998E-4	0.0	0.0	0.00109078018052412	6.059889891800666E-5
13	1.8179669675401998E-4	0.0	0.0	0.0012119779783601333	6.059889891800666E-5
14	1.8179669675401998E-4	0.0	0.0	0.0015755713718681732	6.059889891800666E-5
15	1.8179669675401998E-4	0.0	0.0	0.00218156036104824	6.059889891800666E-5
16	1.8179669675401998E-4	0.0	0.0	0.002848148249146313	6.059889891800666E-5
17	1.8179669675401998E-4	0.0	0.0	0.003696532833998406	6.059889891800666E-5
18	1.8179669675401998E-4	0.0	0.0	0.003938928429670433	6.059889891800666E-5
19	1.8179669675401998E-4	0.0	0.0	0.005150906408030566	6.059889891800666E-5
20	1.8179669675401998E-4	0.0	0.0	0.00872624144419296	6.059889891800666E-5
21	1.8179669675401998E-4	0.0	0.0	0.016240504910025783	6.059889891800666E-5
22	1.8179669675401998E-4	0.0	0.0	0.027451301209857018	6.059889891800666E-5
23	1.8179669675401998E-4	0.0	0.0	0.03429897678759177	6.059889891800666E-5
24	1.8179669675401998E-4	0.0	0.0	0.044176597311226855	6.059889891800666E-5
25	1.8179669675401998E-4	0.0	0.0	0.04902450922466739	6.059889891800666E-5
26	1.8179669675401998E-4	0.0	0.0	0.05865973415263045	6.059889891800666E-5
27	1.8179669675401998E-4	0.0	0.0	0.11925863307063711	6.059889891800666E-5
28	1.8179669675401998E-4	0.0	0.0	0.2700892924775557	6.059889891800666E-5
29	1.8179669675401998E-4	0.0	0.0	0.4989107347919488	6.059889891800666E-5
30	1.8179669675401998E-4	0.0	0.0	0.8070561357900127	6.059889891800666E-5
31	1.8179669675401998E-4	0.0	0.0	1.2590633228194243	6.059889891800666E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7150	0.0	21.294407	1
GTCGACG	60	9.239142E-4	18.5	36
GTATTGG	545	0.0	17.651377	1
ATTGGAC	585	0.0	17.393162	3
TTGGACC	755	0.0	17.152319	4
TAGTACT	270	0.0	17.12963	4
TAGCACA	170	8.54925E-11	16.32353	4
CTGTCCG	80	3.3836826E-4	16.1875	9
CGTCTAT	70	0.0025931357	15.857142	27
TATACTG	175	1.3278623E-10	15.857142	5
GTATACT	130	2.5907684E-7	15.653846	4
GTCTTAC	95	7.062654E-5	15.578948	1
GACCCTC	755	0.0	15.437087	7
GGACCCT	770	0.0	15.136363	6
CGAACTA	125	2.9604507E-6	14.800001	29
CGTTTAG	90	8.2767074E-4	14.388888	26
TGGACCC	775	0.0	14.322579	5
CCCTCGC	785	0.0	14.140127	9
CTAGTAC	265	0.0	13.962263	3
TTATGGG	80	0.0063001546	13.875	2
>>END_MODULE