FastQCFastQC Report
Wed 25 May 2016
SRR2088543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2692355
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT523221.9433544239151228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT369641.3729244471847137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT368981.3704730616876304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146430.5438733005120053No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG97730.3629907645908508No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA86010.31946010091536964No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG80480.29892046182617077No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA79080.2937205531959938No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA74680.27737798321543783No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA65100.24179575130322709No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG57580.21386481351827677No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47930.1780225861745572No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC47380.1759797649269877No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT41020.15235732286418394No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA40570.15068592366162709No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39330.1460802903034704No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT36790.1366461703601494No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33400.12405496303422098No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG33350.12386925201171464No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG31840.11826077913202383No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT31310.11629224229345685No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA30890.11473226970440376No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA30640.11380371459187218No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT29320.10890094359770534No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27880.10355246614952338No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT27190.10098965403893617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118800.023.4987371
ATTGGAC8900.022.4494363
GTATTGG9200.022.3206521
TTGGACC13950.020.422944
TGGACCC14000.019.8214285
GGACCCT13800.019.7065226
GACCCTC13950.019.627247
TATTGGA10300.019.2184472
CTGTGCG1951.8189894E-1216.1282069
CCCTCGC16300.016.1165649
TTATGCG2003.6379788E-1215.7254
ACCCTCG16900.015.4349128
TACTCCG2400.015.4166675
CCTCGCA17500.014.90571510
GTATTAG4350.014.8850581
CGTGCGA750.0041063114.810
GTCTAGT1001.09422996E-414.81
GTATCAA192400.014.5480772
CTCGCAT17750.014.48732411
TCGCATG18050.014.349030512
Produced by FastQC (version 0.11.5)