FastQCFastQC Report
Wed 25 May 2016
SRR2088542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3732411
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT575511.5419255810788255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT412271.1045675302103655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT401271.0750959634402535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167130.4477802685717088No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG101310.2714331299527303No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA91270.24453362719164634No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA82490.22100995844241164No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG81900.21942921077019653No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA75300.2017462707081294No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG65620.17581129195043096No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA63010.16881849292588624No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51460.1378733478172688No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC51070.13682844681360118No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT42890.1149123180700089No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA41660.11161686105844186No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA39220.10507953170216248No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT37700.10100709702120159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124400.023.6752411
GTATTGG10150.020.2315251
TTGGACC14200.017.7183114
ATTGGAC11950.017.029293
GGACCCT14900.015.8926176
GACCCTC15000.015.2933327
TGGACCC16750.015.1313435
CAATGCG1601.0988515E-815.0312519
ACGTTCG2151.2732926E-1114.62790722
GTATCAA203750.014.4912892
TATACCG2450.014.3469385
TATACTG5050.014.2871295
TATACAC20950.014.04057237
TTAGAAC3050.013.950823
TACTCTT63450.013.7620175
CCGTTTA1501.3076351E-613.56666727
TTTACTC62950.013.4892773
TTACTCT63200.013.465194
CGTTTAT3300.013.4545461
CAACCGT1102.45868E-413.4545456
Produced by FastQC (version 0.11.5)