##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088542_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3732411
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.739874306446957	31.0	31.0	34.0	30.0	34.0
2	31.895358522949376	31.0	31.0	34.0	30.0	34.0
3	32.018667022468854	33.0	31.0	34.0	30.0	34.0
4	35.68891314488142	37.0	35.0	37.0	35.0	37.0
5	35.59603671728542	37.0	35.0	37.0	33.0	37.0
6	35.6434382494318	37.0	35.0	37.0	33.0	37.0
7	35.50574789325184	37.0	35.0	37.0	33.0	37.0
8	35.542892784315555	37.0	35.0	37.0	33.0	37.0
9	37.15280632277635	39.0	37.0	39.0	33.0	39.0
10	37.007796033180696	39.0	37.0	39.0	33.0	39.0
11	37.071832657228796	39.0	37.0	39.0	33.0	39.0
12	36.925829175832995	39.0	37.0	39.0	33.0	39.0
13	36.98592866648394	39.0	37.0	39.0	33.0	39.0
14	38.018446253641415	40.0	37.0	41.0	33.0	41.0
15	38.023422125805546	40.0	37.0	41.0	33.0	41.0
16	37.92052911643439	40.0	37.0	41.0	33.0	41.0
17	37.942880352672844	40.0	37.0	41.0	33.0	41.0
18	37.91064247747636	40.0	37.0	41.0	33.0	41.0
19	37.97015709148859	40.0	37.0	41.0	33.0	41.0
20	37.86036746756989	40.0	37.0	41.0	32.0	41.0
21	37.74018081074137	40.0	37.0	41.0	32.0	41.0
22	37.620279224340514	40.0	37.0	41.0	32.0	41.0
23	37.458376100595565	39.0	36.0	41.0	32.0	41.0
24	37.608708419303234	39.0	37.0	41.0	32.0	41.0
25	37.56040452136702	39.0	36.0	41.0	32.0	41.0
26	37.363182404081435	39.0	36.0	41.0	31.0	41.0
27	37.315717374104835	39.0	36.0	41.0	31.0	41.0
28	37.17105377730373	39.0	36.0	41.0	31.0	41.0
29	36.953050186595206	39.0	36.0	41.0	31.0	41.0
30	36.80408508066234	39.0	35.0	41.0	30.0	41.0
31	36.56697132229007	39.0	35.0	40.0	30.0	41.0
32	36.41712314104743	38.0	35.0	40.0	30.0	41.0
33	36.261336439100624	38.0	35.0	40.0	30.0	41.0
34	36.078419284478585	38.0	35.0	40.0	30.0	41.0
35	35.88692831523645	38.0	35.0	40.0	29.0	41.0
36	35.82161717988721	38.0	35.0	40.0	28.0	41.0
37	35.69293520997554	38.0	35.0	40.0	27.0	41.0
38	35.55659625909365	38.0	35.0	40.0	26.0	41.0
39	35.41419634654383	38.0	35.0	40.0	25.0	41.0
40	35.23767961245426	38.0	34.0	40.0	25.0	41.0
41	35.10978292583534	38.0	34.0	40.0	24.0	41.0
42	34.84125971121615	38.0	34.0	40.0	23.0	41.0
43	34.46357890382383	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	0.0
11	2.0
12	1.0
13	8.0
14	12.0
15	13.0
16	28.0
17	108.0
18	249.0
19	524.0
20	1219.0
21	2475.0
22	4608.0
23	7814.0
24	12755.0
25	19761.0
26	29087.0
27	41485.0
28	56435.0
29	74004.0
30	93926.0
31	115562.0
32	142835.0
33	176666.0
34	219737.0
35	273429.0
36	358599.0
37	519742.0
38	701745.0
39	879580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06498453680476	21.20977566511298	14.6795730695253	23.045666728556956
2	18.377799229506074	23.701784181859928	35.682431543578666	22.23798504505533
3	18.985958405974046	24.1401335490652	33.56733757348802	23.306570471472728
4	13.362488750569002	16.441168992375168	36.32242001215836	33.87392224489747
5	12.330715990280813	38.80896289288613	34.96737095673547	13.89295016009759
6	31.864122145176406	38.30558853245262	13.780208021035198	16.050081301335787
7	25.494057326484143	34.14393538117855	21.156458921592506	19.205548370744808
8	27.209999113173765	34.630376986885956	19.027111430118495	19.132512469821787
9	24.746765562527813	14.599651538911443	21.874332703445575	38.77925019511517
10	16.187445594817934	28.477678369289983	32.93932527795037	22.395550757941717
11	33.355463800744346	22.869855436606525	23.295746368768068	20.478934393881058
12	21.2889738027243	27.858266412782516	30.735280760880833	20.11747902361235
13	31.788112295242943	21.34550562625606	25.861540971774012	21.004841106726992
14	20.385375565552668	23.14214592122893	28.522234019779706	27.950244493438692
15	22.511079299680556	30.308398512382478	25.254667827310552	21.925854360626413
16	21.54384927061891	28.61879358945196	26.418419622061982	23.418937517867136
17	21.43893049291731	27.38310437944803	27.340183061297374	23.837782066337283
18	22.727641730774025	25.9655756024725	29.847409623431076	21.4593730433224
19	22.67001142157174	25.2634289203413	31.261964451396164	20.804595206690795
20	23.284386419394863	25.21587252850771	30.466767995271688	21.032973056825735
21	22.317933368002613	25.725221579295525	31.085938820778313	20.87090623192355
22	21.090013934692617	26.308919355344308	30.757277266624712	21.84378944333837
23	22.07222087814016	26.197811548620987	31.06624645570919	20.66372111752966
24	21.659913659026298	26.339623369452074	29.759503977455857	22.240958994065764
25	21.76459130572705	26.392618604971425	30.65602903860266	21.186761050698866
26	21.1315956361719	27.368556142396965	30.48024453898566	21.019603682445474
27	20.349179123092284	27.20490856982256	30.359357530561343	22.086554776523805
28	20.930733512466876	27.0058683247906	30.152226000834315	21.911172161908215
29	20.997044537699626	26.88393641536262	29.560035055089056	22.5589839918487
30	21.214116023128213	27.281481058758	31.086447875113432	20.417955043000354
31	22.029353144656362	26.982987672043617	30.681321001358103	20.306338181941914
32	21.240613640887887	26.982612579375637	29.99053426860011	21.78623951113637
33	20.982871393316547	26.579548715294216	30.635613280531004	21.801966610858237
34	20.310624955290294	26.875577207333272	31.43247086132797	21.381326976048456
35	19.84205383597894	28.54846371420511	30.514672687439838	21.094809762376116
36	21.77924671211182	26.684012023327547	30.35536547288067	21.18137579167996
37	20.965402791921896	27.195959930457818	30.35054285286374	21.488094424756547
38	20.635883883098618	25.915125638628755	31.65827664745389	21.79071383081874
39	20.169375773461176	25.70694920789806	32.36639801993939	21.757276998701375
40	19.027004260784786	25.88069749017458	32.505021553092625	22.58727669594801
41	19.98158830846871	25.183159089392888	32.639733405565465	22.19551919657294
42	19.125894763465222	26.69462178736479	31.977694846575044	22.201788602594945
43	19.010768106727795	26.575690619280675	31.640620499725248	22.772920774266286
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1591.0
1	1407.5
2	1224.0
3	2642.0
4	4060.0
5	4060.0
6	5738.0
7	7416.0
8	7961.0
9	8506.0
10	12845.5
11	17185.0
12	17185.0
13	30690.5
14	44196.0
15	75661.0
16	107126.0
17	98122.0
18	89118.0
19	89118.0
20	91066.5
21	93015.0
22	75735.5
23	58456.0
24	66152.5
25	73849.0
26	73849.0
27	80987.0
28	88125.0
29	94448.0
30	100771.0
31	109769.5
32	118768.0
33	118768.0
34	130701.0
35	142634.0
36	155946.5
37	169259.0
38	177501.0
39	185743.0
40	185743.0
41	191612.5
42	197482.0
43	206873.5
44	216265.0
45	229706.5
46	243148.0
47	243148.0
48	307617.5
49	372087.0
50	345823.0
51	319559.0
52	290239.0
53	260919.0
54	260919.0
55	236027.0
56	211135.0
57	186734.5
58	162334.0
59	145815.5
60	129297.0
61	129297.0
62	113500.5
63	97704.0
64	84543.0
65	71382.0
66	60340.5
67	49299.0
68	49299.0
69	41879.0
70	34459.0
71	28610.5
72	22762.0
73	18638.0
74	14514.0
75	14514.0
76	11463.0
77	8412.0
78	6599.5
79	4787.0
80	3828.5
81	2870.0
82	2870.0
83	2158.5
84	1447.0
85	1092.0
86	737.0
87	569.5
88	402.0
89	402.0
90	309.0
91	216.0
92	154.5
93	93.0
94	66.5
95	40.0
96	40.0
97	28.5
98	17.0
99	9.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3732411.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.92025615829206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.46219950191282	35.87625987056181
2	12.636881825867263	11.858514646235149
3	4.498098182971066	6.331557569104515
4	2.0704654955430066	3.885870856711319
5	1.0852747339310806	2.546068425908428
6	0.685223896816529	1.9290528458648777
7	0.4669679036116101	1.5337177558610167
8	0.3078101271607966	1.1554024011600823
9	0.23573483715645396	0.9954665050332677
>10	1.3414171315447272	11.697681406917564
>50	0.12120990217846894	3.955911019953711
>100	0.07880538197019293	6.913049830102935
>500	0.005156052838390799	1.6106717027882738
>1k	0.004010263311172257	3.7443816175281524
>5k	4.5831580699111515E-4	1.510999292771558
>10k+	2.8644737936944694E-4	4.4553942534973485
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	57551	1.5419255810788255	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	41227	1.1045675302103655	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	40127	1.0750959634402535	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16713	0.4477802685717088	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	10131	0.2714331299527303	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9127	0.24453362719164634	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8249	0.22100995844241164	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	8190	0.21942921077019653	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7530	0.2017462707081294	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6562	0.17581129195043096	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6301	0.16881849292588624	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5146	0.1378733478172688	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5107	0.13682844681360118	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	4289	0.1149123180700089	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4166	0.11161686105844186	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3922	0.10507953170216248	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3770	0.10100709702120159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	2.6792333427374425E-5	0.0	0.0	2.6792333427374425E-5
4	0.0	5.358466685474885E-5	0.0	0.0	2.6792333427374425E-5
5	0.0	8.037700028212328E-5	0.0	0.0	2.6792333427374425E-5
6	0.0	8.037700028212328E-5	0.0	0.0	5.358466685474885E-5
7	0.0	8.037700028212328E-5	0.0	0.0	5.358466685474885E-5
8	2.6792333427374425E-5	8.037700028212328E-5	0.0	2.6792333427374425E-5	5.358466685474885E-5
9	2.6792333427374425E-5	8.037700028212328E-5	0.0	1.071693337094977E-4	5.358466685474885E-5
10	8.037700028212328E-5	8.037700028212328E-5	0.0	2.143386674189954E-4	5.358466685474885E-5
11	1.071693337094977E-4	8.037700028212328E-5	0.0	3.7509266798324195E-4	8.037700028212328E-5
12	1.071693337094977E-4	8.037700028212328E-5	0.0	6.162236688296118E-4	8.037700028212328E-5
13	1.071693337094977E-4	8.037700028212328E-5	0.0	6.430160022569862E-4	8.037700028212328E-5
14	1.071693337094977E-4	8.037700028212328E-5	0.0	7.501853359664839E-4	8.037700028212328E-5
15	1.071693337094977E-4	8.037700028212328E-5	0.0	0.001071693337094977	8.037700028212328E-5
16	1.071693337094977E-4	8.037700028212328E-5	0.0	0.0014735783385055933	8.037700028212328E-5
17	1.071693337094977E-4	8.037700028212328E-5	0.0	0.0019290480067709584	8.037700028212328E-5
18	1.071693337094977E-4	1.071693337094977E-4	0.0	0.002277348341326826	8.037700028212328E-5
19	1.071693337094977E-4	1.071693337094977E-4	0.0	0.003107910677575433	8.037700028212328E-5
20	1.071693337094977E-4	1.071693337094977E-4	0.0	0.005197712684910638	8.037700028212328E-5
21	1.071693337094977E-4	1.071693337094977E-4	0.0	0.009029016365025181	8.037700028212328E-5
22	1.071693337094977E-4	1.6075400056424655E-4	0.0	0.014708991051628558	8.037700028212328E-5
23	1.071693337094977E-4	1.6075400056424655E-4	0.0	0.018058032730050362	8.037700028212328E-5
24	1.071693337094977E-4	1.6075400056424655E-4	0.0	0.023845176750363236	8.037700028212328E-5
25	1.071693337094977E-4	1.8754633399162098E-4	0.0	0.02748893409648616	8.037700028212328E-5
26	1.071693337094977E-4	1.8754633399162098E-4	0.0	0.03493720278929625	8.037700028212328E-5
27	1.071693337094977E-4	2.143386674189954E-4	0.0	0.08964714764799482	8.037700028212328E-5
28	1.071693337094977E-4	2.143386674189954E-4	0.0	0.23011935180771892	8.037700028212328E-5
29	1.071693337094977E-4	2.143386674189954E-4	0.0	0.43376787818919194	8.037700028212328E-5
30	1.071693337094977E-4	2.411310008463698E-4	0.0	0.7055225161430507	8.037700028212328E-5
31	1.071693337094977E-4	2.411310008463698E-4	0.0	1.11584710258329	8.037700028212328E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12440	0.0	23.675241	1
GTATTGG	1015	0.0	20.231525	1
TTGGACC	1420	0.0	17.718311	4
ATTGGAC	1195	0.0	17.02929	3
GGACCCT	1490	0.0	15.892617	6
GACCCTC	1500	0.0	15.293332	7
TGGACCC	1675	0.0	15.131343	5
CAATGCG	160	1.0988515E-8	15.03125	19
ACGTTCG	215	1.2732926E-11	14.627907	22
GTATCAA	20375	0.0	14.491289	2
TATACCG	245	0.0	14.346938	5
TATACTG	505	0.0	14.287129	5
TATACAC	2095	0.0	14.040572	37
TTAGAAC	305	0.0	13.95082	3
TACTCTT	6345	0.0	13.762017	5
CCGTTTA	150	1.3076351E-6	13.566667	27
TTTACTC	6295	0.0	13.489277	3
TTACTCT	6320	0.0	13.46519	4
CGTTTAT	330	0.0	13.454546	1
CAACCGT	110	2.45868E-4	13.454545	6
>>END_MODULE