FastQCFastQC Report
Wed 25 May 2016
SRR2088541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2878913
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT549371.908254955950388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT386201.341478537211788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT381411.3248403129931332No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156000.5418711854092153No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG94160.3270678898598186No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA84440.29330514676893676No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA77990.27090085737220954No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG76550.26589896950689373No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA68780.23890961623362705No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG60590.2104613789996433No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA59580.20695311042744258No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49200.17089783539829095No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC49120.17061995273910674No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39070.13571094367908998No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT39010.13550253168470183No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA35900.12469984330891555No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT35380.12289360602421816No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33280.11559918622063257No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG30650.10646379379995158No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG30090.10451861518566208No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA29950.10403232053208972No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA29390.10208714191780022No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA29310.10180925925861602No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29210.10146190593463575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132350.022.0294671
GTATTGG9650.020.1295341
ATTGGAC10100.019.2326743
CGTATAC1355.638867E-1119.1851863
CGTAAAT500.007037390518.515
CGTTCGC2650.017.45283123
GGACCCT13900.017.035976
TTGGACC14600.016.8527414
TGGACCC14550.016.6563595
CTAGTAC2650.016.0566043
TTAACGG1751.3278623E-1015.85714335
GACCCTC15100.015.5596047
CCGTATA1601.0984877E-815.031252
CTAATAC5800.014.9913793
GTATTAG5250.014.8000011
CGGTATA1508.11433E-814.826
TAGTACT3000.014.84
TTCGTTA2750.014.79999930
ACGTTCG2950.014.42372922
TATTGGA13350.014.4119842
Produced by FastQC (version 0.11.5)