FastQCFastQC Report
Wed 25 May 2016
SRR2088540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4433599
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT581271.311056773515151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT421580.9508753498004668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT408790.922027454445023No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173980.39241257497577026No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG98270.22164837189831554No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA90910.20504786292129712No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA79900.1802147645738823No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG77910.17572631173906345No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG76230.17193706512474402No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA68100.15359981811616252No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA64200.14480335276149242No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC53200.11999280945344853No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50100.11300074724845437No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46850.10567035945289595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143250.021.8771381
GTATTGG12800.019.3671881
ATTGGAC13500.017.6777783
CGTTCGC3000.016.6523
GGACCCT17600.016.3977286
TTGGACC19650.015.8167944
GACCCTC18350.015.6267027
TATACCG2201.8189894E-1215.1363645
TGGACCC20150.014.965265
CGAACTA3100.014.91935524
ACGTTCG3000.014.822
TATTGGA16050.014.177572
GTATTAG8250.014.1272741
GTATCAA224500.013.9677072
GTATAGA5600.013.8751
TAATACT9600.013.6822924
TATACAC22500.013.56666737
CCCTCGC20150.013.4962779
CTAATAC9800.013.2142853
ACCCTCG21350.012.9976588
Produced by FastQC (version 0.11.5)