##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088540_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4433599
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75430953498501	31.0	31.0	34.0	30.0	34.0
2	31.91555483479674	31.0	31.0	34.0	30.0	34.0
3	32.04626173905218	33.0	31.0	34.0	30.0	34.0
4	35.709339748588	37.0	35.0	37.0	35.0	37.0
5	35.61256329225986	37.0	35.0	37.0	33.0	37.0
6	35.65312447066142	37.0	35.0	37.0	33.0	37.0
7	35.528097827521165	37.0	35.0	37.0	33.0	37.0
8	35.552454788987454	37.0	35.0	37.0	33.0	37.0
9	37.17427263945161	39.0	37.0	39.0	33.0	39.0
10	37.027951783641235	39.0	37.0	39.0	33.0	39.0
11	37.08230063205987	39.0	37.0	39.0	33.0	39.0
12	36.94126194091978	39.0	37.0	39.0	33.0	39.0
13	37.00053568218506	39.0	37.0	39.0	33.0	39.0
14	38.06753520108607	40.0	37.0	41.0	33.0	41.0
15	38.06698711362936	40.0	37.0	41.0	33.0	41.0
16	37.96433461844429	40.0	37.0	41.0	33.0	41.0
17	37.96943634279961	40.0	37.0	41.0	33.0	41.0
18	37.92572287209556	40.0	37.0	41.0	33.0	41.0
19	37.9775293164763	40.0	37.0	41.0	33.0	41.0
20	37.87415506003136	40.0	37.0	41.0	32.0	41.0
21	37.73961515238523	40.0	37.0	41.0	32.0	41.0
22	37.6237560049973	39.0	37.0	41.0	32.0	41.0
23	37.476463252540434	39.0	36.0	41.0	32.0	41.0
24	37.64332543380671	39.0	37.0	41.0	32.0	41.0
25	37.599898863203464	40.0	37.0	41.0	32.0	41.0
26	37.41640775361056	39.0	36.0	41.0	32.0	41.0
27	37.37460604804359	39.0	36.0	41.0	32.0	41.0
28	37.245721139868536	39.0	36.0	41.0	31.0	41.0
29	37.03866272073771	39.0	36.0	41.0	31.0	41.0
30	36.91284011025805	39.0	36.0	41.0	31.0	41.0
31	36.70927569227619	39.0	35.0	40.0	30.0	41.0
32	36.56449782670918	39.0	35.0	40.0	30.0	41.0
33	36.41620408160504	39.0	35.0	40.0	30.0	41.0
34	36.24813430353083	38.0	35.0	40.0	30.0	41.0
35	36.05993031846137	38.0	35.0	40.0	29.0	41.0
36	36.003700379759195	38.0	35.0	40.0	29.0	41.0
37	35.88279544451359	38.0	35.0	40.0	28.0	41.0
38	35.75532563950867	38.0	35.0	40.0	27.0	41.0
39	35.63281252995591	38.0	35.0	40.0	26.0	41.0
40	35.47636446146799	38.0	35.0	40.0	26.0	41.0
41	35.36149773581237	38.0	34.0	40.0	25.0	41.0
42	35.113153219314604	38.0	34.0	40.0	24.0	41.0
43	34.750191210346266	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	2.0
13	11.0
14	16.0
15	19.0
16	53.0
17	132.0
18	288.0
19	599.0
20	1345.0
21	2758.0
22	5000.0
23	8808.0
24	14350.0
25	21859.0
26	32594.0
27	46496.0
28	63449.0
29	83606.0
30	107431.0
31	133457.0
32	164642.0
33	205442.0
34	256822.0
35	321858.0
36	423145.0
37	610170.0
38	850172.0
39	1079070.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58601172546277	20.822925122456947	14.019490711722012	23.571572440358274
2	18.319045091809162	23.380215486335143	35.53361501570169	22.767124406154007
3	18.97573506309434	24.255666784479153	32.99718806324162	23.77141008918488
4	13.388693925634682	16.333502420945152	36.5867098039313	33.691093849488865
5	12.547639062531365	38.5283152581007	35.08431863143239	13.839727047935549
6	32.078002543757336	38.08463507863476	14.095726744795819	15.741635632812079
7	26.10398459581031	33.46867860625194	21.490058076970875	18.937278720966873
8	27.16395867104806	34.576559585113586	19.175730597196544	19.083751146641813
9	24.893342857574623	14.616838374422226	21.403582056022657	39.08623671198049
10	16.150445721410527	28.11499190612412	33.37809756813821	22.35646480432714
11	33.70126617224516	22.82096328513246	23.14341012797955	20.33436041464282
12	21.308873445704045	27.34534629766923	30.83368613174083	20.5120941248859
13	31.437213875228682	21.112802488452385	25.7317136709928	21.718269965326137
14	20.707759091428883	22.519650514175954	28.12579576998281	28.646794624412358
15	22.815301970250353	29.953475720289546	24.87590781214088	22.35531449731922
16	22.014034196597393	28.37947229778787	26.12584042896076	23.48065307665398
17	21.783499139186922	27.268884714201715	27.110187457187717	23.83742868942365
18	22.82051218434504	25.997637585176285	29.23205278600974	21.94979744446893
19	22.821076060329318	25.341150609245446	30.471948410309547	21.36582492011569
20	23.43601665373887	25.288281596959944	29.80650708374844	21.46919466555275
21	22.51926707850665	25.839053103359145	30.308943140775703	21.332736677358508
22	21.630778967606226	26.235232369909863	29.919056730209476	22.214931932274435
23	22.345142174562923	26.24208910187863	30.27741119573511	21.13535752782333
24	22.033837521165083	26.293086045896345	28.983315811826916	22.689760621111652
25	22.15423632132721	26.296379081644506	29.885742937058584	21.6636416599697
26	21.657664574536398	27.15419233900044	29.792477849259708	21.395665237203453
27	20.842164571040367	27.14079464561409	29.585445142873766	22.431595640471773
28	21.385898905155834	26.905635805132576	29.58706910570848	22.12139618400311
29	21.532326220752037	26.816092298829915	29.06938584206646	22.582195638351596
30	21.542633873744556	27.16472554238667	30.163282696518113	21.12935788735066
31	22.21937527503051	26.835602407885784	29.95221263808477	20.992809678998935
32	21.5950066751639	26.854097540169963	29.394246074126233	22.156649710539902
33	21.368396194603978	26.62780283016123	29.97079799052643	22.03300298470836
34	20.888154296317733	26.89158401560448	30.473369377789915	21.746892310287873
35	20.49783934000346	28.40832019314331	29.701400600279822	21.392439866573408
36	22.154822752350857	26.882449224659243	29.386780356094448	21.575947666895452
37	21.32283501507466	27.212429450656227	29.62640509437141	21.8383304398977
38	21.015522603645483	26.228172642586756	30.690935287562095	22.065369466205674
39	20.669032088828963	26.021884252500055	31.2991544792391	22.00992917943188
40	19.838645759348104	26.223120313767662	31.36546629498969	22.57276763189454
41	20.5874054013455	25.419416595862636	31.554545190036354	22.438632812755507
42	19.754695902809434	26.801408968199425	30.939942922217366	22.503952206773775
43	19.717412422729254	26.521343044330354	30.71660292236623	23.044641610574164
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1322.0
1	1289.0
2	1256.0
3	2738.0
4	4220.0
5	4220.0
6	6164.5
7	8109.0
8	8611.5
9	9114.0
10	13488.0
11	17862.0
12	17862.0
13	32103.0
14	46344.0
15	78010.5
16	109677.0
17	101241.0
18	92805.0
19	92805.0
20	94920.5
21	97036.0
22	79808.0
23	62580.0
24	70692.0
25	78804.0
26	78804.0
27	88960.0
28	99116.0
29	108447.0
30	117778.0
31	129663.0
32	141548.0
33	141548.0
34	156933.0
35	172318.0
36	188377.0
37	204436.0
38	216409.5
39	228383.0
40	228383.0
41	238969.5
42	249556.0
43	260978.0
44	272400.0
45	285575.0
46	298750.0
47	298750.0
48	361134.0
49	423518.0
50	398927.5
51	374337.0
52	342603.5
53	310870.0
54	310870.0
55	283137.5
56	255405.0
57	225961.5
58	196518.0
59	177750.5
60	158983.0
61	158983.0
62	141284.0
63	123585.0
64	107285.5
65	90986.0
66	77343.0
67	63700.0
68	63700.0
69	54524.0
70	45348.0
71	38378.0
72	31408.0
73	25427.0
74	19446.0
75	19446.0
76	15583.0
77	11720.0
78	9146.0
79	6572.0
80	5159.0
81	3746.0
82	3746.0
83	2856.0
84	1966.0
85	1501.5
86	1037.0
87	790.0
88	543.0
89	543.0
90	419.5
91	296.0
92	202.5
93	109.0
94	86.5
95	64.0
96	64.0
97	43.5
98	23.0
99	14.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4433599.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.01692686247508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.69858322082104	34.83416167665505
2	12.793243761909286	11.774115250511905
3	4.567123862471992	6.30495014253715
4	2.104077733532706	3.8729276470774745
5	1.1757510143950105	2.705222421894804
6	0.7367137167230348	2.034078073261565
7	0.4966139461313016	1.5996853346606472
8	0.36275262275271275	1.335420872830608
9	0.27587374002006526	1.1425375546002727
>10	1.56128051999363	13.535629762470377
>50	0.1406934500784312	4.486537215181185
>100	0.07828733720939345	6.660851077850154
>500	0.004970013607075024	1.5278383089331822
>1k	0.003395355664115001	3.1037271377777467
>5k	4.4287247792804043E-4	1.491834235266753
>10k+	1.968322124124624E-4	3.590483288491043
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	58127	1.311056773515151	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	42158	0.9508753498004668	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	40879	0.922027454445023	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17398	0.39241257497577026	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9827	0.22164837189831554	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9091	0.20504786292129712	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7990	0.1802147645738823	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7791	0.17572631173906345	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7623	0.17193706512474402	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6810	0.15359981811616252	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6420	0.14480335276149242	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5320	0.11999280945344853	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5010	0.11300074724845437	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4685	0.10567035945289595	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.2555039370948975E-5	0.0	0.0	0.0	0.0
7	4.511007874189795E-5	0.0	0.0	0.0	0.0
8	4.511007874189795E-5	0.0	0.0	0.0	0.0
9	4.511007874189795E-5	0.0	0.0	4.511007874189795E-5	0.0
10	6.766511811284692E-5	0.0	0.0	4.511007874189795E-5	0.0
11	6.766511811284692E-5	0.0	0.0	1.3533023622569384E-4	0.0
12	6.766511811284692E-5	0.0	0.0	2.0299535433854077E-4	0.0
13	6.766511811284692E-5	0.0	0.0	2.2555039370948974E-4	0.0
14	9.02201574837959E-5	0.0	0.0	2.932155118223367E-4	0.0
15	9.02201574837959E-5	0.0	0.0	4.0599070867708154E-4	0.0
16	9.02201574837959E-5	0.0	0.0	6.992062204994182E-4	0.0
17	9.02201574837959E-5	0.0	0.0	9.92421732321755E-4	0.0
18	9.02201574837959E-5	0.0	0.0	0.0011277519685474486	0.0
19	1.1277519685474487E-4	0.0	0.0	0.00175929307093402	0.0
20	1.1277519685474487E-4	0.0	0.0	0.0030223752757071627	0.0
21	1.1277519685474487E-4	0.0	0.0	0.005368099370285856	0.0
22	1.3533023622569384E-4	0.0	0.0	0.009179901023976233	0.0
23	1.3533023622569384E-4	0.0	0.0	0.011593290236667773	0.0
24	1.3533023622569384E-4	0.0	0.0	0.015450201969100047	0.0
25	1.3533023622569384E-4	0.0	0.0	0.017863591181791587	0.0
26	1.3533023622569384E-4	0.0	0.0	0.02287080992214226	0.0
27	1.3533023622569384E-4	0.0	0.0	0.06405631181349508	0.0
28	1.3533023622569384E-4	0.0	0.0	0.17015521701443906	0.0
29	1.3533023622569384E-4	0.0	0.0	0.3286043685953556	0.0
30	1.3533023622569384E-4	0.0	0.0	0.5373963680522302	0.0
31	1.3533023622569384E-4	0.0	0.0	0.8457237562531027	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14325	0.0	21.877138	1
GTATTGG	1280	0.0	19.367188	1
ATTGGAC	1350	0.0	17.677778	3
CGTTCGC	300	0.0	16.65	23
GGACCCT	1760	0.0	16.397728	6
TTGGACC	1965	0.0	15.816794	4
GACCCTC	1835	0.0	15.626702	7
TATACCG	220	1.8189894E-12	15.136364	5
TGGACCC	2015	0.0	14.96526	5
CGAACTA	310	0.0	14.919355	24
ACGTTCG	300	0.0	14.8	22
TATTGGA	1605	0.0	14.17757	2
GTATTAG	825	0.0	14.127274	1
GTATCAA	22450	0.0	13.967707	2
GTATAGA	560	0.0	13.875	1
TAATACT	960	0.0	13.682292	4
TATACAC	2250	0.0	13.566667	37
CCCTCGC	2015	0.0	13.496277	9
CTAATAC	980	0.0	13.214285	3
ACCCTCG	2135	0.0	12.997658	8
>>END_MODULE