FastQCFastQC Report
Wed 25 May 2016
SRR2088539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1885721
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT366001.9409021801210253No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT254281.348449744156214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT242511.2860332997299175No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107960.5725131130214914No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG57140.30301407260140817No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA53190.2820671774880802No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA46030.24409761571303495No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG42810.22702191893710683No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA42060.22304466037128506No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG39760.21084773410276494No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA36270.19234022424314096No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34340.18210541220042625No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC28830.15288581927018896No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA23900.12674197296418718No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT23630.12531015988049135No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.11846927514727787No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT21400.11348444441144792No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA21160.11221172167038497No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA20280.10754507161982074No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19890.10547689716559343No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG18960.10054509654397442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88750.020.9076061
GTATTGG6200.018.7983881
ATTGGAC6350.018.354333
CGAACTA1750.017.97142824
TTGGACC8850.017.7683624
ACGTTTA1258.587449E-917.76000226
TGTTACG752.0681512E-417.26666616
ACGTTCG1950.017.07692322
GTTCGGA1107.814124E-716.8181821
TTAACGG1107.814124E-716.81818235
GGACCCT9050.015.7403326
CTACGAC957.063509E-515.5789483
GTATTAG3350.015.4626871
ACGAACT855.366415E-415.23529313
TGGACCC9750.015.1794875
ATACCAT2109.094947E-1214.976196
ATAAGCG750.004105702514.83
TTCGTTA2006.184564E-1114.79999930
AGGCTCG1406.0002094E-714.53571424
TATACAC8150.014.52760737
Produced by FastQC (version 0.11.5)