FastQCFastQC Report
Wed 25 May 2016
SRR2088538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4263026
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT991762.326422592777994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT710741.667219482123731No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT704511.6526054497439144No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285300.6692429274416811No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA196770.46157353954679137No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG185070.4341282459924007No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG157480.36940895973892723No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA152100.3567888162070792No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG152090.3567653586912207No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA136480.32014817643617466No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA122060.2863224385682846No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA99090.23244052464141668No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC97930.22971945280183606No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT88060.20656688464954237No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA80410.18862188501782537No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT70820.16612612730956836No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT62730.1471489969800794No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC61010.14311430425242538No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60890.14283281406212395No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA60690.14236366374495488No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA59410.13936110171507282No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA56510.13255842211612126No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA55740.13075219339502034No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG54610.1281014941030151No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG53500.12549770984272673No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT51980.1219321674322418No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT50960.11953950081467953No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT50000.11728757929226799No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC49900.11705300413368344No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG49290.11562209566631779No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48790.1144492198733951No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG48170.11299485389017097No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT47080.11043798466159954No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA46200.10837372326605561No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44890.10530078868859818No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC43500.10204019398427314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG17900.022.5307271
ATTGGAC18850.021.78783
GGTATCA247600.020.5696681
TTGGACC26200.019.3473284
TATTGGA21200.019.2853782
GGACCCT26900.018.4312296
TGGACCC27700.018.032495
TATACCG3250.017.6461545
GACCCTC28550.016.653247
ACCGTTA3050.015.1639348
GATACTG21900.015.12100436
TGATACT22450.014.91536835
ACTCTGT20800.014.67548137
GTCTATA1904.5656634E-1014.6052641
GATACCT22050.014.5147436
ATACTGT23250.014.40215137
TATACAC21950.014.32801937
CCCTCGC32500.014.2307699
GACCGTT3650.014.1917827
ACGTTTA2353.6379788E-1214.17021226
Produced by FastQC (version 0.11.5)