##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088538_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4263026
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.710555366070956	31.0	31.0	34.0	30.0	34.0
2	31.84747805901254	31.0	31.0	34.0	30.0	34.0
3	31.954079097805174	33.0	31.0	34.0	30.0	34.0
4	35.61969666617093	37.0	35.0	37.0	33.0	37.0
5	35.56097359012119	37.0	35.0	37.0	33.0	37.0
6	35.62997879909717	37.0	35.0	37.0	33.0	37.0
7	35.463094759450215	37.0	35.0	37.0	33.0	37.0
8	35.525685276139534	37.0	35.0	37.0	33.0	37.0
9	37.103877855776624	39.0	37.0	39.0	33.0	39.0
10	36.98719219634128	39.0	37.0	39.0	33.0	39.0
11	37.050150526879264	39.0	37.0	39.0	33.0	39.0
12	36.89191527332932	39.0	37.0	39.0	32.0	39.0
13	36.957749260736385	39.0	37.0	39.0	33.0	39.0
14	37.90294945421398	40.0	37.0	41.0	33.0	41.0
15	37.919696009360486	40.0	37.0	41.0	33.0	41.0
16	37.80669834056841	40.0	37.0	41.0	32.0	41.0
17	37.897325749362075	40.0	37.0	41.0	33.0	41.0
18	37.8902411104225	40.0	37.0	41.0	33.0	41.0
19	37.97026783322457	40.0	37.0	41.0	33.0	41.0
20	37.87862072621654	40.0	37.0	41.0	32.0	41.0
21	37.756986000085384	40.0	37.0	41.0	32.0	41.0
22	37.640383849406504	40.0	37.0	41.0	32.0	41.0
23	37.45729793813127	39.0	36.0	41.0	32.0	41.0
24	37.582151739163685	39.0	36.0	41.0	32.0	41.0
25	37.53086469564108	40.0	36.0	41.0	32.0	41.0
26	37.30236456451357	39.0	36.0	41.0	31.0	41.0
27	37.2598009019884	39.0	36.0	41.0	31.0	41.0
28	37.088257026816166	39.0	35.0	41.0	31.0	41.0
29	36.84426437933993	39.0	35.0	41.0	30.0	41.0
30	36.627395892025994	39.0	35.0	40.0	30.0	41.0
31	36.31924248174888	38.0	35.0	40.0	30.0	41.0
32	36.155251926683064	38.0	35.0	40.0	30.0	41.0
33	35.95109764753956	38.0	35.0	40.0	30.0	41.0
34	35.720993960627965	38.0	35.0	40.0	29.0	41.0
35	35.479512674799544	38.0	35.0	40.0	27.0	41.0
36	35.35234948133087	38.0	34.0	40.0	26.0	41.0
37	35.15703282128704	38.0	34.0	40.0	25.0	41.0
38	34.98616217681994	38.0	34.0	40.0	24.0	41.0
39	34.795965823337696	38.0	34.0	40.0	24.0	41.0
40	34.54371800688056	38.0	33.0	40.0	23.0	41.0
41	34.341248211950855	38.0	33.0	40.0	21.0	41.0
42	33.95927728331941	38.0	33.0	40.0	19.0	41.0
43	33.536824546695236	37.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	7.0
13	10.0
14	11.0
15	34.0
16	54.0
17	122.0
18	273.0
19	704.0
20	1450.0
21	3036.0
22	5682.0
23	10040.0
24	16445.0
25	25373.0
26	37283.0
27	52660.0
28	71765.0
29	93455.0
30	116708.0
31	140652.0
32	171614.0
33	211570.0
34	260185.0
35	321548.0
36	424753.0
37	636458.0
38	766720.0
39	894411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.35015221582041	20.790654338021866	15.339268397612399	21.51992504854533
2	18.38958992978227	24.528984810320182	35.60466204053177	21.476763219365775
3	19.78411579005148	24.011113232713104	34.50466874938131	21.700102227854114
4	12.864195526839387	15.687659423142152	35.77672291935353	35.67142213066493
5	11.882920723448555	39.36602779340309	34.38548111130451	14.36557037184385
6	31.967386546551673	37.361958383551965	12.497413808876605	18.173241261019754
7	24.92145250814797	34.73917353541827	20.22858880053746	20.110785155896306
8	28.524831891712598	33.35454674684133	18.15553083654662	19.96509052489945
9	24.07428432291992	14.377627534995094	22.33995288792515	39.208135254159835
10	16.697106703078987	28.278011909849955	31.91538123389348	23.109500153177578
11	32.64408896403634	22.588672928572333	23.609379816121226	21.1578582912701
12	22.306056777509685	28.248385067320726	30.294326142979187	19.151232012190402
13	33.62566871513334	20.639564478377565	26.23582403672884	19.498942769760262
14	20.22556278099172	23.458665276730663	29.269420360091637	27.046351582185984
15	22.125832683169186	30.303193083973685	26.270025094850464	21.300949138006665
16	20.8219701216929	28.394337730992024	27.20600343511862	23.577688712196455
17	21.419245390480846	26.60424778080171	27.329601086176815	24.64690574254063
18	23.25737633314927	24.4094453095055	31.16377896827277	21.169399389072456
19	22.419192376495005	23.656412135417423	33.2936979507045	20.63069753738307
20	23.610013169049402	23.33321448191965	32.398324570387324	20.65844777864362
21	22.08726852709789	24.456102308547965	33.57626718673543	19.880361977618715
22	20.196147056105218	25.173315855920187	32.829074934096106	21.80146215387849
23	21.8425597216625	25.187460737982832	33.60936574161171	19.36061379874296
24	21.206837584382548	25.329988604338794	31.181348647650754	22.281825163627904
25	21.24580051822344	25.612252892663566	32.443104968161116	20.698841620951878
26	20.563046061647288	27.06424966678599	32.434378772261766	19.938325499304955
27	19.192306122458554	26.775065411282974	32.173671941010916	21.858956525247557
28	20.19431736986826	26.409878804398566	31.85734733965967	21.538456486073507
29	20.324623870461966	26.25142328477471	30.87192055596189	22.55203228880143
30	20.204497931750826	26.792189397859644	33.517975259827175	19.485337410562355
31	21.66327392795634	26.50903372393225	32.7469267135598	19.080765634551607
32	20.84852402964467	26.344432335153478	31.29530525969112	21.511738375510728
33	20.277122400848597	26.260290225769207	32.31589485966072	21.146692513721472
34	19.290405453778607	27.292420923541165	32.97577823827488	20.44139538440535
35	18.48989426759302	30.20089485731497	31.41702630948064	19.892184565611377
36	21.685652398085303	27.191014082485072	31.154325589381816	19.96900793004781
37	20.497951455140083	28.53620878690395	30.81022728925416	20.155612468701808
38	20.15361857985384	26.33971737446593	32.55312071753726	20.953543328142967
39	19.650736354880312	26.012508485756364	33.38332911879965	20.953426040563674
40	18.044881734242296	26.693034478325963	33.07594183099047	22.18614195644127
41	19.98739862248084	25.549973188059372	33.27441587266885	21.188212316790935
42	18.630475160132733	27.984253438754536	32.2886372262332	21.096634174879533
43	18.686585538066154	28.11326977597603	31.303890710495313	21.8962539754625
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1251.0
1	1179.5
2	1108.0
3	3035.0
4	4962.0
5	4962.0
6	7884.5
7	10807.0
8	11706.5
9	12606.0
10	19201.5
11	25797.0
12	25797.0
13	48106.0
14	70415.0
15	124549.5
16	178684.0
17	160540.0
18	142396.0
19	142396.0
20	140619.5
21	138843.0
22	102956.5
23	67070.0
24	72088.0
25	77106.0
26	77106.0
27	83171.0
28	89236.0
29	92122.5
30	95009.0
31	100529.0
32	106049.0
33	106049.0
34	116589.0
35	127129.0
36	140382.5
37	153636.0
38	163002.5
39	172369.0
40	172369.0
41	178747.0
42	185125.0
43	198777.5
44	212430.0
45	238986.5
46	265543.0
47	265543.0
48	385719.5
49	505896.0
50	466518.0
51	427140.0
52	381389.0
53	335638.0
54	335638.0
55	293707.0
56	251776.0
57	208091.5
58	164407.0
59	145389.0
60	126371.0
61	126371.0
62	110786.5
63	95202.0
64	82828.5
65	70455.0
66	60429.0
67	50403.0
68	50403.0
69	43145.5
70	35888.0
71	30594.5
72	25301.0
73	20607.0
74	15913.0
75	15913.0
76	12740.0
77	9567.0
78	7520.5
79	5474.0
80	4261.5
81	3049.0
82	3049.0
83	2295.5
84	1542.0
85	1142.0
86	742.0
87	550.0
88	358.0
89	358.0
90	285.0
91	212.0
92	142.5
93	73.0
94	51.0
95	29.0
96	29.0
97	20.0
98	11.0
99	9.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4263026.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.93126402922119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.92041381185649	28.418259974760385
2	12.63472149931269	9.58501914252214
3	4.747511898013283	5.402373818562325
4	2.3066124688552763	3.499709062769729
5	1.279390915233296	2.426445730115055
6	0.8235943740329176	1.874398539265425
7	0.5343508804101026	1.418802303035768
8	0.4006660037091596	1.215821437938004
9	0.29464484789178175	1.00586263682096
>10	1.751103826744587	12.585630724050215
>50	0.17229456553343667	4.530934581157148
>100	0.11661762800679165	8.360101987200261
>500	0.009690913209274059	2.5238726673094773
>1k	0.006709093752769803	5.704032338729905
>5k	9.939398152251562E-4	2.507943826383798
>10k+	6.833336229672949E-4	8.940791229379359
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	99176	2.326422592777994	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	71074	1.667219482123731	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	70451	1.6526054497439144	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28530	0.6692429274416811	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	19677	0.46157353954679137	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	18507	0.4341282459924007	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15748	0.36940895973892723	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	15210	0.3567888162070792	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	15209	0.3567653586912207	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	13648	0.32014817643617466	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	12206	0.2863224385682846	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	9909	0.23244052464141668	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	9793	0.22971945280183606	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8806	0.20656688464954237	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	8041	0.18862188501782537	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	7082	0.16612612730956836	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	6273	0.1471489969800794	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	6101	0.14311430425242538	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6089	0.14283281406212395	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6069	0.14236366374495488	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5941	0.13936110171507282	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	5651	0.13255842211612126	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5574	0.13075219339502034	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	5461	0.1281014941030151	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	5350	0.12549770984272673	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	5198	0.1219321674322418	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	5096	0.11953950081467953	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	5000	0.11728757929226799	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4990	0.11705300413368344	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4929	0.11562209566631779	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4879	0.1144492198733951	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4817	0.11299485389017097	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	4708	0.11043798466159954	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA	4620	0.10837372326605561	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4489	0.10530078868859818	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	4350	0.10204019398427314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.3457515858453595E-5	2.3457515858453595E-5	0.0	0.0	0.0
3	2.3457515858453595E-5	2.3457515858453595E-5	0.0	0.0	0.0
4	2.3457515858453595E-5	2.3457515858453595E-5	0.0	0.0	0.0
5	4.691503171690719E-5	4.691503171690719E-5	0.0	0.0	0.0
6	9.383006343381438E-5	4.691503171690719E-5	0.0	0.0	0.0
7	9.383006343381438E-5	4.691503171690719E-5	0.0	0.0	0.0
8	1.1728757929226798E-4	4.691503171690719E-5	0.0	0.0	0.0
9	1.4074509515072157E-4	4.691503171690719E-5	0.0	7.037254757536078E-5	0.0
10	1.6420261100917518E-4	4.691503171690719E-5	0.0	7.037254757536078E-5	0.0
11	1.8766012686762876E-4	7.037254757536078E-5	0.0	2.3457515858453596E-4	0.0
12	1.8766012686762876E-4	7.037254757536078E-5	0.0	3.7532025373525753E-4	0.0
13	1.8766012686762876E-4	7.037254757536078E-5	0.0	3.7532025373525753E-4	0.0
14	2.3457515858453596E-4	7.037254757536078E-5	0.0	5.629803806028863E-4	0.0
15	2.3457515858453596E-4	7.037254757536078E-5	0.0	9.85215666055051E-4	0.0
16	2.3457515858453596E-4	9.383006343381438E-5	0.0	0.0016185685942332981	0.0
17	2.3457515858453596E-4	9.383006343381438E-5	0.0	0.002369209101703813	0.0
18	2.3457515858453596E-4	9.383006343381438E-5	0.0	0.002885274450589792	0.0
19	2.580326744429896E-4	9.383006343381438E-5	0.0	0.00417543782280474	0.0
20	2.580326744429896E-4	9.383006343381438E-5	0.0	0.006685392019659275	0.0
21	2.580326744429896E-4	1.1728757929226798E-4	0.0	0.012244823278112778	0.0
22	2.580326744429896E-4	1.1728757929226798E-4	0.0	0.019282078035648857	0.0
23	2.580326744429896E-4	1.1728757929226798E-4	0.0	0.02362171846946277	0.0
24	2.580326744429896E-4	1.1728757929226798E-4	0.0	0.029908332719528335	0.0
25	2.580326744429896E-4	1.1728757929226798E-4	0.0	0.03459983589121905	0.0
26	2.580326744429896E-4	1.6420261100917518E-4	0.0	0.04266922134652709	0.0
27	2.580326744429896E-4	2.8149019030144313E-4	0.0	0.08808297204849325	0.0
28	2.580326744429896E-4	2.8149019030144313E-4	0.0	0.20792742056933267	0.0
29	2.580326744429896E-4	2.8149019030144313E-4	0.0	0.40382113550327864	0.0
30	2.580326744429896E-4	2.8149019030144313E-4	0.0	0.6692194699258226	0.0
31	2.580326744429896E-4	2.8149019030144313E-4	0.0	0.986974979744435	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	1790	0.0	22.530727	1
ATTGGAC	1885	0.0	21.7878	3
GGTATCA	24760	0.0	20.569668	1
TTGGACC	2620	0.0	19.347328	4
TATTGGA	2120	0.0	19.285378	2
GGACCCT	2690	0.0	18.431229	6
TGGACCC	2770	0.0	18.03249	5
TATACCG	325	0.0	17.646154	5
GACCCTC	2855	0.0	16.65324	7
ACCGTTA	305	0.0	15.163934	8
GATACTG	2190	0.0	15.121004	36
TGATACT	2245	0.0	14.915368	35
ACTCTGT	2080	0.0	14.675481	37
GTCTATA	190	4.5656634E-10	14.605264	1
GATACCT	2205	0.0	14.51474	36
ATACTGT	2325	0.0	14.402151	37
TATACAC	2195	0.0	14.328019	37
CCCTCGC	3250	0.0	14.230769	9
GACCGTT	365	0.0	14.191782	7
ACGTTTA	235	3.6379788E-12	14.170212	26
>>END_MODULE