FastQCFastQC Report
Wed 25 May 2016
SRR2088537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5299778
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT931131.7569226484581053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT664821.25442990253554No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT655041.2359762993846157No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266490.5028323827903735No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA150870.284672301368095No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG147880.2790305556194995No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA121830.2298775533616691No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG118470.22353766516257847No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG116180.21921672945546022No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA103720.19570631071716588No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA102560.19351753979128938No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT81740.15423287541478153No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC81540.1538555011172166No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA73600.1388737415038894No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA60310.11379721943070067No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT59230.11175939822385013No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57130.10779696809941851No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT53420.10079667487958931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA230700.020.921761
GTATTGG17450.019.7191981
ATTGGAC18400.018.2989143
TTGGACC26100.016.4444454
GGACCCT25050.016.395216
TATACCG3750.015.7866685
GACCCTC26150.015.2810727
TGGACCC28400.015.1778165
TATACTG6450.014.6279065
CGTTCGC5450.014.5963323
TATTGGA23350.014.5781582
TAGATGT9900.014.2020214
CCGAGTT5200.013.87513
TACACTG9450.013.7037045
ATACTGG10050.013.437816
GTATCAA359600.013.4325642
TATACAC24900.013.37349437
TACGTTA1805.1706593E-813.36111119
CTCTAAT8350.013.2934131
GGACCGT6450.013.1937986
Produced by FastQC (version 0.11.5)