##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088537_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5299778
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.745078378754734	31.0	31.0	34.0	30.0	34.0
2	31.89308212532676	31.0	31.0	34.0	30.0	34.0
3	32.013445280160795	33.0	31.0	34.0	30.0	34.0
4	35.68053190152493	37.0	35.0	37.0	35.0	37.0
5	35.59968379807607	37.0	35.0	37.0	33.0	37.0
6	35.65128916720663	37.0	35.0	37.0	33.0	37.0
7	35.51333697373739	37.0	35.0	37.0	33.0	37.0
8	35.553091469114364	37.0	35.0	37.0	33.0	37.0
9	37.160634652998674	39.0	37.0	39.0	33.0	39.0
10	37.019355905096404	39.0	37.0	39.0	33.0	39.0
11	37.08402672715725	39.0	37.0	39.0	33.0	39.0
12	36.938790077622116	39.0	37.0	39.0	33.0	39.0
13	36.99928506439326	39.0	37.0	39.0	33.0	39.0
14	38.01566895820919	40.0	37.0	41.0	33.0	41.0
15	38.02193091861584	40.0	37.0	41.0	33.0	41.0
16	37.91699935355783	40.0	37.0	41.0	33.0	41.0
17	37.95742727336881	40.0	37.0	41.0	33.0	41.0
18	37.9292543951841	40.0	37.0	41.0	33.0	41.0
19	37.998035955468325	40.0	37.0	41.0	33.0	41.0
20	37.89622093604675	40.0	37.0	41.0	32.0	41.0
21	37.769996214935794	40.0	37.0	41.0	32.0	41.0
22	37.65118897433062	40.0	37.0	41.0	32.0	41.0
23	37.49095471546167	39.0	36.0	41.0	32.0	41.0
24	37.6358432372073	39.0	37.0	41.0	32.0	41.0
25	37.58827426356349	40.0	37.0	41.0	32.0	41.0
26	37.38057178998818	39.0	36.0	41.0	32.0	41.0
27	37.33781075358251	39.0	36.0	41.0	31.0	41.0
28	37.1898662547752	39.0	36.0	41.0	31.0	41.0
29	36.96190444203512	39.0	36.0	41.0	31.0	41.0
30	36.79841419772677	39.0	35.0	41.0	30.0	41.0
31	36.5536284727398	39.0	35.0	40.0	30.0	41.0
32	36.39994392217938	38.0	35.0	40.0	30.0	41.0
33	36.23241879188147	38.0	35.0	40.0	30.0	41.0
34	36.03230191906152	38.0	35.0	40.0	30.0	41.0
35	35.8266227377826	38.0	35.0	40.0	29.0	41.0
36	35.7492521384858	38.0	35.0	40.0	27.0	41.0
37	35.59746427114494	38.0	35.0	40.0	27.0	41.0
38	35.449223156139745	38.0	35.0	40.0	26.0	41.0
39	35.29459233952819	38.0	34.0	40.0	25.0	41.0
40	35.10148538297265	38.0	34.0	40.0	24.0	41.0
41	34.95345163514396	38.0	34.0	40.0	24.0	41.0
42	34.647564860263955	38.0	33.0	40.0	23.0	41.0
43	34.25811081143399	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	8.0
13	7.0
14	21.0
15	39.0
16	68.0
17	148.0
18	360.0
19	808.0
20	1772.0
21	3388.0
22	6522.0
23	11050.0
24	18396.0
25	28025.0
26	40762.0
27	58175.0
28	80224.0
29	104969.0
30	133321.0
31	165201.0
32	202896.0
33	251744.0
34	315368.0
35	393034.0
36	518155.0
37	761228.0
38	992869.0
39	1211218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99953281061962	20.721584941859827	14.475681811577768	22.803200435942788
2	18.438677997455745	23.76173115175768	35.35861313436148	22.440977716425103
3	19.249277988625185	24.20893856308698	33.656409759050284	22.885373689237547
4	13.116606016327475	16.17218683499573	36.44237551082328	34.26883163785351
5	12.325855913209951	38.88300604289462	34.80791082192499	13.983227221970429
6	31.93703585319989	37.92609803655927	13.519830453275592	16.617035656965253
7	25.634243547559915	33.94345951849304	21.07599601341792	19.346300920529124
8	27.846222992736674	33.96774355454134	18.684990201476364	19.501043251245616
9	24.76809405978892	14.415037761959084	21.690059470415555	39.12680870783644
10	16.56127860450004	28.22016318419375	32.604573248162474	22.61398496314374
11	33.383134161468654	22.825371175924726	23.221350026359595	20.57014463624703
12	21.714343506463855	27.78099384540258	30.39687700126307	20.10778564687049
13	32.178008210909965	20.87527062454314	25.93629393533088	21.010427229216017
14	20.573522136210233	22.86635402464028	28.514043418422432	28.046080420727055
15	22.65810001852908	30.098675831327277	25.382138648071674	21.861085502071973
16	21.566186357239868	28.18878451135123	26.683532781939167	23.56149634946973
17	21.635642096706693	27.021565808982945	27.274746225219243	24.06804586909112
18	22.841107684133185	25.359420715358265	30.06020252169053	21.73926907881802
19	22.663722895562795	24.66429725924369	31.641476303347044	21.03050354184647
20	23.381243516237852	24.58436938301944	30.920880082146834	21.11350701859587
21	22.305085231871978	25.35777913716386	31.536924754206687	20.80021087675748
22	21.080222605550645	25.972389786892965	31.021922050319844	21.92546555723655
23	22.111209186498	25.86353617076036	31.59826694627586	20.426987696465776
24	21.61590542094405	26.081186796880928	29.84819364131856	22.454714140856467
25	21.783421871633113	26.00984418592628	31.047923139422068	21.15881080301854
26	21.170849043110863	27.033868211083558	30.94890012374103	20.846382622064546
27	20.236923131497207	26.97879043235396	30.70660695598948	22.077679480159357
28	20.947160428229257	26.70372230685889	30.60041382865471	21.74870343625714
29	21.067750385016126	26.768064624593706	29.807437217181548	22.356747773208614
30	21.00078531591323	27.12604188326379	31.492904042395736	20.38026875842724
31	21.908729007139545	26.849785028731393	31.060697259394637	20.180788704734425
32	21.18528360999272	26.78685786461244	30.16913161268264	21.858726912712193
33	20.863836183326924	26.567754347446254	30.91178536157552	21.6566241076513
34	20.247791511267078	27.12172094755667	31.45827995059416	21.172207590582097
35	19.729449044846785	29.117785688381666	30.433840813709555	20.718924453061994
36	21.923522079604087	27.12798536089625	30.030352214753147	20.918140344746515
37	20.906687034815423	27.805787336752598	30.04620193525087	21.241323693181112
38	20.613599286611628	26.39782270125277	31.38701281449902	21.601565197636578
39	20.238772265555273	26.212097940706197	32.02456782152007	21.524561972218457
40	19.150519134952447	26.539413537699126	31.98411329682111	22.325954030527317
41	20.27109437414171	25.58590190004185	32.20744340612003	21.93556031969641
42	19.282845432393582	27.3789581374918	31.371597074443496	21.966599355671125
43	19.308016298041164	27.04856693997371	31.051847832116742	22.591568929868384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1323.0
1	1366.5
2	1410.0
3	3365.5
4	5321.0
5	5321.0
6	8212.5
7	11104.0
8	11855.0
9	12606.0
10	18985.5
11	25365.0
12	25365.0
13	46675.5
14	67986.0
15	119142.5
16	170299.0
17	154109.5
18	137920.0
19	137920.0
20	138907.5
21	139895.0
22	108583.5
23	77272.0
24	85770.0
25	94268.0
26	94268.0
27	103821.5
28	113375.0
29	121307.5
30	129240.0
31	140559.0
32	151878.0
33	151878.0
34	170114.0
35	188350.0
36	207748.0
37	227146.0
38	240159.5
39	253173.0
40	253173.0
41	264583.0
42	275993.0
43	293553.0
44	311113.0
45	330937.5
46	350762.0
47	350762.0
48	447300.0
49	543838.0
50	510052.0
51	476266.0
52	430777.5
53	385289.0
54	385289.0
55	344464.0
56	303639.0
57	263560.0
58	223481.0
59	201263.0
60	179045.0
61	179045.0
62	157819.0
63	136593.0
64	118176.5
65	99760.0
66	84698.5
67	69637.0
68	69637.0
69	59610.5
70	49584.0
71	42045.0
72	34506.0
73	28428.5
74	22351.0
75	22351.0
76	17845.0
77	13339.0
78	10462.5
79	7586.0
80	5992.0
81	4398.0
82	4398.0
83	3302.0
84	2206.0
85	1710.0
86	1214.0
87	935.0
88	656.0
89	656.0
90	503.0
91	350.0
92	248.5
93	147.0
94	100.5
95	54.0
96	54.0
97	40.5
98	27.0
99	20.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	5299778.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.944977726145055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.32296830012417	28.555613672631765
2	13.312300535834687	10.368944957036618
3	5.127827652825352	5.991094011683821
4	2.4790802831433827	3.8619090563337766
5	1.4167391381794945	2.758743709007918
6	0.857399915393693	2.003485236444362
7	0.5925286796106992	1.6153211360678574
8	0.4199671370850251	1.3084488639591367
9	0.31826904076964446	1.1155482633325822
>10	1.8271209369425803	13.530526854381442
>50	0.1815263026037602	4.919353804894843
>100	0.12740736086592827	9.280491393480121
>500	0.010303516464207085	2.7007755909935214
>1k	0.0056377719870904655	4.613433035158199
>5k	3.888118611786166E-4	0.980613358136462
>10k+	5.346163091205977E-4	6.395697056457542
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	93113	1.7569226484581053	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	66482	1.25442990253554	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	65504	1.2359762993846157	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26649	0.5028323827903735	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	15087	0.284672301368095	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	14788	0.2790305556194995	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	12183	0.2298775533616691	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	11847	0.22353766516257847	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11618	0.21921672945546022	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	10372	0.19570631071716588	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10256	0.19351753979128938	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8174	0.15423287541478153	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	8154	0.1538555011172166	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7360	0.1388737415038894	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6031	0.11379721943070067	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	5923	0.11175939822385013	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5713	0.10779696809941851	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	5342	0.10079667487958931	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8868714878245843E-5
2	0.0	0.0	0.0	0.0	1.8868714878245843E-5
3	0.0	0.0	0.0	0.0	1.8868714878245843E-5
4	0.0	0.0	0.0	1.8868714878245843E-5	1.8868714878245843E-5
5	0.0	0.0	0.0	1.8868714878245843E-5	3.7737429756491686E-5
6	3.7737429756491686E-5	0.0	0.0	1.8868714878245843E-5	3.7737429756491686E-5
7	5.660614463473753E-5	0.0	0.0	1.8868714878245843E-5	3.7737429756491686E-5
8	9.434357439122922E-5	0.0	0.0	1.8868714878245843E-5	3.7737429756491686E-5
9	1.1321228926947506E-4	0.0	0.0	5.660614463473753E-5	3.7737429756491686E-5
10	1.1321228926947506E-4	0.0	0.0	1.3208100414772092E-4	9.434357439122922E-5
11	1.1321228926947506E-4	0.0	0.0	2.2642457853895012E-4	9.434357439122922E-5
12	1.1321228926947506E-4	0.0	0.0	2.45293293417196E-4	9.434357439122922E-5
13	1.1321228926947506E-4	0.0	0.0	2.6416200829544183E-4	9.434357439122922E-5
14	1.5094971902596674E-4	0.0	0.0	3.018994380519335E-4	9.434357439122922E-5
15	1.5094971902596674E-4	0.0	0.0	4.339804421996544E-4	1.5094971902596674E-4
16	1.5094971902596674E-4	0.0	0.0	8.113547397645712E-4	1.5094971902596674E-4
17	1.5094971902596674E-4	0.0	0.0	0.0011698603224512424	1.5094971902596674E-4
18	1.5094971902596674E-4	0.0	0.0	0.0014906284753814216	1.5094971902596674E-4
19	1.698184339042126E-4	0.0	0.0	0.0021321647812417802	1.5094971902596674E-4
20	1.698184339042126E-4	0.0	0.0	0.003245418959058285	1.5094971902596674E-4
21	1.698184339042126E-4	0.0	0.0	0.005773826752743228	1.5094971902596674E-4
22	1.698184339042126E-4	0.0	0.0	0.009547569728392397	1.8868714878245843E-4
23	1.698184339042126E-4	0.0	0.0	0.011830684228660144	1.8868714878245843E-4
24	1.698184339042126E-4	0.0	0.0	0.015510083629918084	1.8868714878245843E-4
25	1.698184339042126E-4	0.0	0.0	0.01811396628311601	1.8868714878245843E-4
26	1.698184339042126E-4	0.0	0.0	0.023887793035859237	1.8868714878245843E-4
27	1.698184339042126E-4	0.0	0.0	0.06088934291209934	1.8868714878245843E-4
28	1.698184339042126E-4	0.0	0.0	0.1592519535723949	1.8868714878245843E-4
29	1.698184339042126E-4	0.0	0.0	0.3199567981904148	2.075558636607043E-4
30	1.698184339042126E-4	0.0	0.0	0.5313241422565247	2.075558636607043E-4
31	1.698184339042126E-4	0.0	0.0	0.8244684966049521	2.075558636607043E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23070	0.0	20.92176	1
GTATTGG	1745	0.0	19.719198	1
ATTGGAC	1840	0.0	18.298914	3
TTGGACC	2610	0.0	16.444445	4
GGACCCT	2505	0.0	16.39521	6
TATACCG	375	0.0	15.786668	5
GACCCTC	2615	0.0	15.281072	7
TGGACCC	2840	0.0	15.177816	5
TATACTG	645	0.0	14.627906	5
CGTTCGC	545	0.0	14.59633	23
TATTGGA	2335	0.0	14.578158	2
TAGATGT	990	0.0	14.202021	4
CCGAGTT	520	0.0	13.875	13
TACACTG	945	0.0	13.703704	5
ATACTGG	1005	0.0	13.43781	6
GTATCAA	35960	0.0	13.432564	2
TATACAC	2490	0.0	13.373494	37
TACGTTA	180	5.1706593E-8	13.361111	19
CTCTAAT	835	0.0	13.293413	1
GGACCGT	645	0.0	13.193798	6
>>END_MODULE