FastQCFastQC Report
Wed 25 May 2016
SRR2088536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4570130
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT838971.8357683479463385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT586851.2840991394117893No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT580411.2700076365442559No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249250.5453892996479313No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG140230.3068402868189745No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA125280.27412786944791506No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA115040.2517215046399118No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG113210.24771724217910648No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG110900.24266268136792607No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA100270.219402949150243No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA93810.20526768385144403No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC77520.16962318358558728No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT74510.16303693768010974No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA64190.14045552314704396No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA58700.12844273576462814No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT53770.11765529645765001No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53540.11715202849809524No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT49250.10776498699161731No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA46500.101747652692593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG16300.021.3374231
GGTATCA209750.020.3301541
ATTGGAC18100.019.215473
TTGGACC23750.017.764
GTATTAG8000.016.8812481
GGACCCT23500.016.4531926
TATACCG2500.016.2799995
TGGACCC24250.016.2494835
ACGTATA1951.8189894E-1216.12820429
TCTAACG1659.767973E-1015.6969712
GACGTAT2109.094947E-1214.9761928
TATTGGA22800.014.9298242
TATACTG5100.014.8725495
GACCCTC25200.014.8293657
ACGTTCG4850.014.11340222
TAATACT10750.013.9395354
TAGTACT5950.013.6806734
GATACTG20350.013.45454536
TCTACAC5500.013.4545453
ATTAGAG7600.013.3881583
Produced by FastQC (version 0.11.5)