##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4570130
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71963335835086	31.0	31.0	34.0	30.0	34.0
2	31.860621470286404	31.0	31.0	34.0	30.0	34.0
3	31.98148542820445	33.0	31.0	34.0	30.0	34.0
4	35.642225275867425	37.0	35.0	37.0	33.0	37.0
5	35.566335530936755	37.0	35.0	37.0	33.0	37.0
6	35.62618612599642	37.0	35.0	37.0	33.0	37.0
7	35.47796342773619	37.0	35.0	37.0	33.0	37.0
8	35.52383805274686	37.0	35.0	37.0	33.0	37.0
9	37.12167793913959	39.0	37.0	39.0	33.0	39.0
10	36.98162809373038	39.0	37.0	39.0	33.0	39.0
11	37.04843035099658	39.0	37.0	39.0	33.0	39.0
12	36.90376269384022	39.0	37.0	39.0	33.0	39.0
13	36.9615533912602	39.0	37.0	39.0	33.0	39.0
14	37.96561585775459	40.0	37.0	41.0	33.0	41.0
15	37.97174456744119	40.0	37.0	41.0	33.0	41.0
16	37.863562743291766	40.0	37.0	41.0	32.0	41.0
17	37.91330990584513	40.0	37.0	41.0	33.0	41.0
18	37.8935667037918	40.0	37.0	41.0	33.0	41.0
19	37.95933857461385	40.0	37.0	41.0	33.0	41.0
20	37.858407966512985	40.0	37.0	41.0	32.0	41.0
21	37.73476421896095	40.0	37.0	41.0	32.0	41.0
22	37.61863054223841	40.0	37.0	41.0	32.0	41.0
23	37.4551997426769	39.0	36.0	41.0	32.0	41.0
24	37.5946353823633	39.0	37.0	41.0	32.0	41.0
25	37.545960618188104	39.0	36.0	41.0	32.0	41.0
26	37.33627993076784	39.0	36.0	41.0	31.0	41.0
27	37.29158142109743	39.0	36.0	41.0	31.0	41.0
28	37.1363858358515	39.0	36.0	41.0	31.0	41.0
29	36.908543083019524	39.0	35.0	41.0	31.0	41.0
30	36.74355018347399	39.0	35.0	41.0	30.0	41.0
31	36.48860995201449	39.0	35.0	40.0	30.0	41.0
32	36.33451936815802	38.0	35.0	40.0	30.0	41.0
33	36.1603985006991	38.0	35.0	40.0	30.0	41.0
34	35.96715738939592	38.0	35.0	40.0	29.0	41.0
35	35.758635312343415	38.0	35.0	40.0	28.0	41.0
36	35.668493237610306	38.0	35.0	40.0	27.0	41.0
37	35.5115003293123	38.0	35.0	40.0	26.0	41.0
38	35.36044795224643	38.0	34.0	40.0	25.0	41.0
39	35.20800436749064	38.0	34.0	40.0	25.0	41.0
40	35.00562150310822	38.0	34.0	40.0	24.0	41.0
41	34.85246415309849	38.0	34.0	40.0	23.0	41.0
42	34.54595843006654	38.0	33.0	40.0	22.0	41.0
43	34.15327747788356	38.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	6.0
13	3.0
14	20.0
15	21.0
16	64.0
17	117.0
18	286.0
19	707.0
20	1552.0
21	3066.0
22	5746.0
23	9985.0
24	16343.0
25	25328.0
26	37333.0
27	52568.0
28	72346.0
29	94865.0
30	118255.0
31	145944.0
32	178208.0
33	219586.0
34	272973.0
35	338284.0
36	443862.0
37	654278.0
38	844380.0
39	1034000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.05134208436084	20.74317360775295	14.629999584256904	22.575484723629305
2	18.48743908816598	24.0144153448589	35.29549487651336	22.20265069046176
3	19.45283832188581	24.26738407878988	33.57646281396809	22.703314785356216
4	13.147131482036617	16.131729294352677	36.199954924695795	34.52118429891491
5	12.227245176833044	39.08295825282869	34.702645220157855	13.98715135018041
6	31.94701244822357	37.79794010236033	13.416620533770374	16.838426915645726
7	25.50483246647251	33.98629798277073	21.06176410736675	19.447105443390015
8	27.870126232732982	34.00485325362736	18.69544192397153	19.42957858966813
9	24.582976851861982	14.436460231984649	21.79559443604449	39.18496848010888
10	16.463995553736986	28.279961401535626	32.605155652027406	22.65088739269999
11	33.17629914247516	22.788170139580274	23.271373024399743	20.76415769354482
12	21.60706588215215	27.93038710058576	30.565629424108288	19.896917593153805
13	32.24365608855766	20.929623446160175	26.023526683048402	20.803193782233766
14	20.45828018021369	22.91781634220471	28.756709327743412	27.867194149838188
15	22.516339797773803	30.185005678175457	25.682923680507997	21.615730843542742
16	21.315214228041654	28.25088126595961	26.775233964898153	23.658670541100584
17	21.48630345307464	27.009581784325608	27.418957447599958	24.08515731499979
18	22.778936266583226	25.415841562493846	30.215114230886208	21.590107940036717
19	22.632682221293486	24.636848404749976	31.848196878425778	20.882272495530763
20	23.41029686245249	24.481754348344577	31.156553533488108	20.95139525571483
21	22.16683114047084	25.390065490478392	31.823449223545065	20.61965414550571
22	20.768008787496196	25.9390433094901	31.366044291956683	21.926903611057018
23	21.806622568723427	25.990114066777092	31.998695879548283	20.204567484951195
24	21.473459179498175	26.09871053996276	30.082426539288814	22.34540374125025
25	21.583237238328014	26.07144654528427	31.283595871452235	21.061720344935484
26	21.02666663749171	27.263031905000513	31.15342451965261	20.556876937855158
27	20.05750383468304	27.08299326277371	30.90028073599657	21.959222166546684
28	20.61764107366749	26.818777584007456	30.88706448175435	21.676516860570704
29	20.86883742913221	26.777312680383268	30.090391301779164	22.263458588705355
30	20.83463708909812	27.032907160190188	31.848043709916347	20.28441204079534
31	21.638815526035366	26.88249568393022	31.451862419668586	20.026826370365832
32	21.02049613468326	26.751295914995854	30.469680293558387	21.7585276567625
33	20.63770614840278	26.604954344843584	31.247557509304986	21.509781997448652
34	19.905604435760033	27.116405879045015	31.843076673967698	21.13491301122725
35	19.517147214630658	29.19794404097914	30.65127250209513	20.63363624229508
36	21.752116460582087	27.02583952754079	30.402504961565647	20.819539050311477
37	20.742342121558906	27.725972784144	30.436508370659038	21.09517672363806
38	20.40674991739841	26.221595446956652	31.81255237815992	21.559102257485016
39	19.913197217584617	26.021601136072714	32.51557395522666	21.549627691116008
40	18.928083008579623	26.425528376654494	32.35697890432001	22.289409710445874
41	20.083630006148624	25.50028117362088	32.48439322294989	21.931695597280605
42	19.00545498705726	27.30486878929046	31.773603814333505	21.916072409318772
43	19.078778940642827	27.113933301678507	31.270576548150707	22.536711209527958
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1193.0
1	1220.5
2	1248.0
3	3139.5
4	5031.0
5	5031.0
6	7744.0
7	10457.0
8	11166.0
9	11875.0
10	17680.5
11	23486.0
12	23486.0
13	43459.5
14	63433.0
15	109304.5
16	155176.0
17	139632.0
18	124088.0
19	124088.0
20	124222.0
21	124356.0
22	95803.0
23	67250.0
24	73590.5
25	79931.0
26	79931.0
27	89016.0
28	98101.0
29	104552.5
30	111004.0
31	120320.0
32	129636.0
33	129636.0
34	144308.5
35	158981.0
36	174775.0
37	190569.0
38	202529.5
39	214490.0
40	214490.0
41	224520.0
42	234550.0
43	249576.0
44	264602.0
45	283350.5
46	302099.0
47	302099.0
48	392629.5
49	483160.0
50	448988.5
51	414817.0
52	374411.5
53	334006.0
54	334006.0
55	297428.5
56	260851.0
57	225150.5
58	189450.0
59	169626.5
60	149803.0
61	149803.0
62	132249.5
63	114696.0
64	99039.0
65	83382.0
66	70237.0
67	57092.0
68	57092.0
69	48965.0
70	40838.0
71	34694.0
72	28550.0
73	23241.5
74	17933.0
75	17933.0
76	14360.0
77	10787.0
78	8419.0
79	6051.0
80	4786.0
81	3521.0
82	3521.0
83	2629.0
84	1737.0
85	1323.5
86	910.0
87	706.0
88	502.0
89	502.0
90	395.0
91	288.0
92	202.5
93	117.0
94	86.0
95	55.0
96	55.0
97	38.0
98	21.0
99	14.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4570130.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.273232569733835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.15914574684534	30.26901756405224
2	12.834063270826485	10.337384298413479
3	4.551048007263105	5.498562444975923
4	2.1890868988943946	3.5264642317812553
5	1.2361251858626825	2.489137854777664
6	0.747263902035369	1.805683975058233
7	0.5144728639825703	1.4503639711390968
8	0.3703297562809503	1.193150112175645
9	0.28728169438867884	1.0412786242028218
>10	1.801490871223296	13.987898413291644
>50	0.1764783591876009	4.9508074877746004
>100	0.11789520573159538	8.75571046508257
>500	0.008994694661499615	2.432884208687584
>1k	0.005396816713937238	4.717181871165678
>5k	3.8159310098546094E-4	1.0451109808761396
>10k+	5.451330014078013E-4	6.499363496545526
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	83897	1.8357683479463385	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	58685	1.2840991394117893	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	58041	1.2700076365442559	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24925	0.5453892996479313	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	14023	0.3068402868189745	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12528	0.27412786944791506	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	11504	0.2517215046399118	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11321	0.24771724217910648	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	11090	0.24266268136792607	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10027	0.219402949150243	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	9381	0.20526768385144403	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	7752	0.16962318358558728	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7451	0.16303693768010974	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6419	0.14045552314704396	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5870	0.12844273576462814	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	5377	0.11765529645765001	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5354	0.11715202849809524	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	4925	0.10776498699161731	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4650	0.101747652692593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	2.1881215632815697E-5	0.0
7	0.0	0.0	0.0	2.1881215632815697E-5	0.0
8	2.1881215632815697E-5	0.0	0.0	2.1881215632815697E-5	0.0
9	2.1881215632815697E-5	0.0	0.0	1.3128729379689418E-4	0.0
10	8.752486253126279E-5	0.0	0.0	1.9693094069534126E-4	0.0
11	1.3128729379689418E-4	0.0	0.0	3.5009945012505115E-4	0.0
12	1.3128729379689418E-4	0.0	0.0	5.03267959554761E-4	0.0
13	1.3128729379689418E-4	0.0	0.0	5.907928220860239E-4	0.0
14	1.5316850942970986E-4	0.0	0.0	7.439613315157337E-4	0.0
15	1.5316850942970986E-4	0.0	0.0	9.627734878438906E-4	0.0
16	1.7504972506252557E-4	0.0	0.0	0.0014222790161330203	0.0
17	1.7504972506252557E-4	0.0	0.0	0.002231883994547201	0.0
18	1.9693094069534126E-4	0.0	0.0	0.0027570331697347778	0.0
19	1.9693094069534126E-4	0.0	0.0	0.0036322817950474057	0.0
20	1.9693094069534126E-4	0.0	0.0	0.005973571867758685	0.0
21	1.9693094069534126E-4	0.0	0.0	0.011159419972736005	0.0
22	1.9693094069534126E-4	0.0	0.0	0.018402102347198	0.0
23	2.1881215632815697E-4	0.0	0.0	0.022603295748698616	0.0
24	2.4069337196097265E-4	0.0	0.0	0.02929894773234022	0.0
25	2.4069337196097265E-4	0.0	0.0	0.033894003015231516	0.0
26	2.4069337196097265E-4	0.0	0.0	0.042165102524435845	0.0
27	2.4069337196097265E-4	0.0	0.0	0.08938476586005212	0.0
28	2.6257458759378836E-4	0.0	0.0	0.21266353473533575	0.0
29	2.6257458759378836E-4	0.0	0.0	0.39793178749838626	0.0
30	2.6257458759378836E-4	0.0	0.0	0.646568040734071	0.0
31	2.8445580322660407E-4	0.0	0.0	0.9817882642288075	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTGG	1630	0.0	21.337423	1
GGTATCA	20975	0.0	20.330154	1
ATTGGAC	1810	0.0	19.21547	3
TTGGACC	2375	0.0	17.76	4
GTATTAG	800	0.0	16.881248	1
GGACCCT	2350	0.0	16.453192	6
TATACCG	250	0.0	16.279999	5
TGGACCC	2425	0.0	16.249483	5
ACGTATA	195	1.8189894E-12	16.128204	29
TCTAACG	165	9.767973E-10	15.696971	2
GACGTAT	210	9.094947E-12	14.97619	28
TATTGGA	2280	0.0	14.929824	2
TATACTG	510	0.0	14.872549	5
GACCCTC	2520	0.0	14.829365	7
ACGTTCG	485	0.0	14.113402	22
TAATACT	1075	0.0	13.939535	4
TAGTACT	595	0.0	13.680673	4
GATACTG	2035	0.0	13.454545	36
TCTACAC	550	0.0	13.454545	3
ATTAGAG	760	0.0	13.388158	3
>>END_MODULE