FastQCFastQC Report
Wed 25 May 2016
SRR2088535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033887
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT176941.711405598484167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT123551.195004869971283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT120911.169470164534422No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53510.5175613969418321No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG29970.28987694012982074No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA26160.2530257175107144No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA24930.24112886611399506No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG23810.2302959607771449No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG22680.2193663330712157No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21980.21259576723568438No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA19110.18483644731000584No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.15543284710998398No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC14840.1435359957132646No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA12330.11925868107443077No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA11930.11538978631126999No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11610.11229467050074139No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT10730.10378310202178767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG652.6824127E-622.769235
TTAGTAC450.003825551120.5555573
GGTATCA42850.020.4212381
ACGTTTA656.901271E-519.92307726
GTATTGG4350.019.563221
TCGGCTC500.007034414418.529
CGTTTAT701.2190465E-418.527
CTCTAAT1501.2732926E-1118.51
TACACCG500.007034414418.55
ATTGGAC4750.017.5263163
CCGGTCT953.6056754E-617.5263169
TAATACT2450.017.3673464
GGCTAGC752.0670125E-417.2666661
TACTCCG1107.805738E-716.8181825
GGACCCT5400.016.7870376
TCTAATA2450.016.6122442
TATTGGA5250.016.5619052
TAGTACC904.4452598E-516.4444454
TGTACTG2500.016.2799995
GTCTATA803.3821532E-416.18751
Produced by FastQC (version 0.11.5)