##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088535_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1033887
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.703389248534897	31.0	31.0	34.0	30.0	34.0
2	31.858743750525928	31.0	31.0	34.0	30.0	34.0
3	31.978977393080676	33.0	31.0	34.0	30.0	34.0
4	35.65306846879785	37.0	35.0	37.0	33.0	37.0
5	35.56764520687464	37.0	35.0	37.0	33.0	37.0
6	35.61832579382467	37.0	35.0	37.0	33.0	37.0
7	35.47293272862508	37.0	35.0	37.0	33.0	37.0
8	35.51656032042187	37.0	35.0	37.0	33.0	37.0
9	37.119681357827304	39.0	37.0	39.0	33.0	39.0
10	36.977934725942006	39.0	37.0	39.0	33.0	39.0
11	37.035225319594886	39.0	37.0	39.0	33.0	39.0
12	36.894185728227555	39.0	37.0	39.0	33.0	39.0
13	36.94965407244699	39.0	37.0	39.0	33.0	39.0
14	37.96226086603275	40.0	37.0	41.0	33.0	41.0
15	37.97167098532044	40.0	37.0	41.0	33.0	41.0
16	37.858337516575794	40.0	37.0	41.0	32.0	41.0
17	37.89836800346653	40.0	37.0	41.0	33.0	41.0
18	37.87799150197265	40.0	37.0	41.0	33.0	41.0
19	37.947432359629246	40.0	37.0	41.0	33.0	41.0
20	37.84593093829403	40.0	37.0	41.0	32.0	41.0
21	37.71387395334306	40.0	37.0	41.0	32.0	41.0
22	37.58898506316454	39.0	37.0	41.0	32.0	41.0
23	37.425857951594324	39.0	36.0	41.0	32.0	41.0
24	37.575955592825906	39.0	36.0	41.0	32.0	41.0
25	37.52907812942807	39.0	36.0	41.0	32.0	41.0
26	37.32553170704342	39.0	36.0	41.0	31.0	41.0
27	37.2716157568477	39.0	36.0	41.0	31.0	41.0
28	37.12574488314487	39.0	36.0	41.0	31.0	41.0
29	36.90060132296856	39.0	35.0	41.0	30.0	41.0
30	36.733921598782075	39.0	35.0	41.0	30.0	41.0
31	36.49600875143995	39.0	35.0	40.0	30.0	41.0
32	36.34454635758066	38.0	35.0	40.0	30.0	41.0
33	36.16947983677133	38.0	35.0	40.0	30.0	41.0
34	35.97829356593129	38.0	35.0	40.0	29.0	41.0
35	35.77937821057814	38.0	35.0	40.0	28.0	41.0
36	35.69749208569215	38.0	35.0	40.0	27.0	41.0
37	35.550136523623955	38.0	35.0	40.0	27.0	41.0
38	35.411401826311774	38.0	35.0	40.0	26.0	41.0
39	35.26170751735925	38.0	34.0	40.0	25.0	41.0
40	35.072775844942434	38.0	34.0	40.0	24.0	41.0
41	34.92559631758596	38.0	34.0	40.0	24.0	41.0
42	34.63729595207213	38.0	33.0	40.0	22.0	41.0
43	34.2397109161833	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	4.0
15	4.0
16	16.0
17	26.0
18	51.0
19	179.0
20	316.0
21	677.0
22	1245.0
23	2204.0
24	3645.0
25	5593.0
26	8309.0
27	11634.0
28	16086.0
29	21230.0
30	26780.0
31	32661.0
32	40338.0
33	49996.0
34	62499.0
35	77118.0
36	100687.0
37	147698.0
38	194001.0
39	230888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.240193560805	21.21266637456511	14.851139437868936	22.696000626760952
2	18.245417535958957	23.89951706521119	35.741043266817364	22.114022132012494
3	19.212544504380073	24.504322039062295	33.47541849351041	22.807714963047218
4	13.259089242828278	16.427036997273397	36.42641797411129	33.88745578578703
5	12.168641253831415	39.074966606602075	34.88882247286212	13.867569666704387
6	31.659262569313668	38.2063030099034	13.691438232611494	16.442996188171435
7	25.252082674412197	33.93455957952852	21.48484312115347	19.328514624905814
8	27.363338546669024	34.56151397589872	19.03689668213257	19.03825079529968
9	24.520377952329415	14.629355045570744	21.907906763505103	38.942360238594745
10	16.40430724053983	28.29419462668551	32.91181724888696	22.389680883887696
11	33.14569193732004	22.914883347986773	23.36986537213448	20.569559342558712
12	21.224079614116437	28.029175335408997	30.7684495500959	19.978295500378668
13	31.800670672907195	21.128517913466364	26.157113881884577	20.913697531741864
14	20.235867169236098	23.091111504448744	28.77625891417534	27.896762412139818
15	22.40244823660613	30.263945672979737	25.60463570970522	21.72897038070892
16	21.128517913466364	28.550895794221226	26.921704209454223	23.398882082858186
17	21.396535598184325	27.21312870748931	27.57100147308168	23.81933422124468
18	22.648606665912233	25.687043168160546	30.157937956469134	21.50641220945809
19	22.527123370348985	24.988997830517263	31.703948303828174	20.779930495305578
20	23.227296600112005	24.857068519093477	31.01112597411516	20.904508906679357
21	22.0480574763006	25.74749464883493	31.597263530734015	20.607184344130452
22	20.811171820518105	26.22472281787081	31.18870824374424	21.775397117866845
23	21.68505842514704	26.239327895601743	31.765560452931506	20.310053226319706
24	21.47023804342254	26.283530018270856	30.037034995120354	22.209196943186246
25	21.545681491304176	26.241165620614243	31.151663576386973	21.061489311694608
26	21.0108067902972	27.396804486370367	30.932587410422997	20.659801312909437
27	20.115737986839953	27.249786485370258	30.654123709844498	21.980351817945287
28	20.640843728569948	26.962230882098332	30.69426349301229	21.702661896319423
29	20.904895796155675	26.834073743068632	30.020495470007845	22.24053499076785
30	20.894256335556978	27.080232172374735	31.584689622753743	20.440821869314536
31	21.60932481015817	26.88195131576275	31.349654265891726	20.159069608187355
32	20.988463923039944	26.749538392493573	30.526643627398354	21.735354057068133
33	20.60437939542716	26.607840121792808	31.194898475365296	21.592882007414737
34	20.081885157662295	26.959425933395043	31.789160710986792	21.16952819795587
35	19.59469458461128	28.78573770634508	30.739336117003113	20.880231592040523
36	21.632925068213453	26.820145721921257	30.485923510016082	21.061005699849208
37	20.701198486875256	27.3187495345236	30.556917728920084	21.42313424968106
38	20.39826402691977	26.113588815799016	31.77726385959007	21.71088329769114
39	19.900917605115453	25.915211236817946	32.52608844099984	21.65778271706676
40	18.958164673702253	26.28913991567744	32.407603538878035	22.34509187174227
41	19.987000513595778	25.4387568467347	32.515448980401146	22.05879365926837
42	19.11775658268263	27.184305441503763	31.69640395904001	22.001534016773594
43	19.169889939616226	26.935535508232523	31.30883742614038	22.58573712601087
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	302.0
1	305.0
2	308.0
3	712.0
4	1116.0
5	1116.0
6	1662.0
7	2208.0
8	2386.0
9	2564.0
10	3876.5
11	5189.0
12	5189.0
13	9443.5
14	13698.0
15	23309.0
16	32920.0
17	29692.5
18	26465.0
19	26465.0
20	26710.0
21	26955.0
22	21074.5
23	15194.0
24	16960.5
25	18727.0
26	18727.0
27	20727.0
28	22727.0
29	24488.0
30	26249.0
31	28617.0
32	30985.0
33	30985.0
34	34642.0
35	38299.0
36	42319.0
37	46339.0
38	49191.0
39	52043.0
40	52043.0
41	53990.0
42	55937.0
43	58935.0
44	61933.0
45	66132.0
46	70331.0
47	70331.0
48	88632.5
49	106934.0
50	98854.0
51	90774.0
52	82082.5
53	73391.0
54	73391.0
55	65597.5
56	57804.0
57	49606.5
58	41409.0
59	37063.0
60	32717.0
61	32717.0
62	28845.5
63	24974.0
64	21780.5
65	18587.0
66	15718.5
67	12850.0
68	12850.0
69	10902.5
70	8955.0
71	7557.5
72	6160.0
73	4980.5
74	3801.0
75	3801.0
76	3029.5
77	2258.0
78	1784.0
79	1310.0
80	1024.5
81	739.0
82	739.0
83	549.5
84	360.0
85	272.0
86	184.0
87	143.5
88	103.0
89	103.0
90	77.5
91	52.0
92	36.5
93	21.0
94	15.5
95	10.0
96	10.0
97	7.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1033887.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.30888960096898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.93980554574918	47.26557243602394
2	9.185703247004916	10.344735000857243
3	2.588800042495143	4.373173673755285
4	1.1972144274543126	2.696552600968487
5	0.6897065588824289	1.9418305240587446
6	0.4353277019178598	1.4707691704521777
7	0.31318246362395513	1.2344469738412542
8	0.23797599190492394	1.0720131084684361
9	0.17578494185260207	0.890842939586183
>10	1.102455238085458	11.600783009921148
>50	0.0777096242825776	3.0079105799929096
>100	0.0480649743251105	4.907274920764982
>500	0.005340552397252952	2.199334478782019
>1k	0.002239586489170593	2.387818374877355
>5k	1.722758837823533E-4	0.5190825157005373
>10k+	5.168276513470598E-4	4.087859691949289
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	17694	1.711405598484167	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12355	1.195004869971283	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12091	1.169470164534422	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5351	0.5175613969418321	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2997	0.28987694012982074	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2616	0.2530257175107144	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2493	0.24112886611399506	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2381	0.2302959607771449	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2268	0.2193663330712157	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2198	0.21259576723568438	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1911	0.18483644731000584	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1607	0.15543284710998398	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1484	0.1435359957132646	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1233	0.11925868107443077	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1193	0.11538978631126999	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1161	0.11229467050074139	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1073	0.10378310202178767	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.672236907901927E-5	0.0
10	0.0	0.0	0.0	9.672236907901927E-5	0.0
11	0.0	0.0	0.0	5.803342144741156E-4	0.0
12	0.0	0.0	0.0	0.001450835536185289	0.0
13	0.0	0.0	0.0	0.001450835536185289	0.0
14	0.0	0.0	0.0	0.0020311697506594046	0.0
15	0.0	0.0	0.0	0.002418059226975482	0.0
16	0.0	0.0	0.0	0.003578727655923713	0.0
17	0.0	0.0	0.0	0.003965617132239791	0.0
18	0.0	0.0	0.0	0.004739396084871944	0.0
19	0.0	0.0	0.0	0.006577121097373311	0.0
20	0.0	0.0	0.0	0.011800129027640351	0.0
21	0.0	0.0	0.0	0.0210854764592262	0.0
22	0.0	0.0	0.0	0.03269216074870852	0.0
23	0.0	0.0	0.0	0.041010284489504174	0.0
24	0.0	0.0	0.0	0.05223007930267041	0.0
25	0.0	0.0	0.0	0.059484256983596856	0.0
26	0.0	0.0	0.0	0.06847943730794565	0.0
27	0.0	0.0	0.0	0.11664717710929724	0.0
28	0.0	0.0	0.0	0.251091270129134	0.0
29	0.0	0.0	0.0	0.4646542610556086	0.0
30	0.0	0.0	0.0	0.7467934116591078	0.0
31	0.0	0.0	0.0	1.1473207420153266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	65	2.6824127E-6	22.76923	5
TTAGTAC	45	0.0038255511	20.555557	3
GGTATCA	4285	0.0	20.421238	1
ACGTTTA	65	6.901271E-5	19.923077	26
GTATTGG	435	0.0	19.56322	1
TCGGCTC	50	0.0070344144	18.5	29
CGTTTAT	70	1.2190465E-4	18.5	27
CTCTAAT	150	1.2732926E-11	18.5	1
TACACCG	50	0.0070344144	18.5	5
ATTGGAC	475	0.0	17.526316	3
CCGGTCT	95	3.6056754E-6	17.526316	9
TAATACT	245	0.0	17.367346	4
GGCTAGC	75	2.0670125E-4	17.266666	1
TACTCCG	110	7.805738E-7	16.818182	5
GGACCCT	540	0.0	16.787037	6
TCTAATA	245	0.0	16.612244	2
TATTGGA	525	0.0	16.561905	2
TAGTACC	90	4.4452598E-5	16.444445	4
TGTACTG	250	0.0	16.279999	5
GTCTATA	80	3.3821532E-4	16.1875	1
>>END_MODULE