FastQCFastQC Report
Wed 25 May 2016
SRR2088534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520342
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT119142.289647962301717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85921.65122169649961No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT84991.6333488359578892No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32640.6272797506255501No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG26000.49967136998358774No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA21980.4224144889322792No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG20850.40069800246760784No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA20210.38839839951416566No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG17740.34092961936572486No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA17730.3407374380695773No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16880.324402027897037No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC12550.24118752666515483No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.20947761280081176No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA10450.20082945447417277No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA10220.1964092846627795No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT9320.17911296800950144No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT8840.16988826579441985No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA8090.1554746685833548No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA7900.15182322395655165No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG7630.14663432896056824No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT7480.14375160951835525No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7230.1389470771146669No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA7120.136833082857044No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG7070.13587217637630633No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT7010.13471908859942114No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT6580.1264552928650772No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT6460.12414911731130679No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG6340.12184294175753639No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC6300.12107421657294626No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.11281042083860231No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA5740.11031206398868436No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC5700.10954333880409423No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5560.10685280065802875No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGATT400.001929774823.12528
CCCTAGA400.001929774823.1252
GGTATCA28050.022.4901941
GGACCCT2750.022.26
TATACTG502.6993488E-422.25
GTATTGG1700.021.7647061
GACCCTC2900.021.0517237
ATTGGAC1750.020.0857143
GTACGGA656.892852E-519.9230773
TTGGACC3100.019.0967734
TGGACCC3200.018.55
TAGCACC500.007029834718.54
GCCCTAG500.007029834718.51
TATTAAG609.227559E-418.52
GACTTGC650.001578362817.0769238
ACCCTCG3600.016.9583348
TATTGGA2100.016.7380942
TACTGTG904.4384353E-516.4444457
GTTTAAG803.3781168E-416.18751
CGTGTCT803.3781168E-416.187535
Produced by FastQC (version 0.11.5)