##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088534_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	520342
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.71678050205442	31.0	31.0	34.0	30.0	34.0
2	31.868657536773892	31.0	31.0	34.0	30.0	34.0
3	31.9756160371448	33.0	31.0	34.0	30.0	34.0
4	35.64233138973982	37.0	35.0	37.0	33.0	37.0
5	35.587676950928426	37.0	35.0	37.0	33.0	37.0
6	35.65685068666377	37.0	35.0	37.0	33.0	37.0
7	35.48164860803087	37.0	35.0	37.0	33.0	37.0
8	35.546623182445394	37.0	35.0	37.0	33.0	37.0
9	37.125317579591886	39.0	37.0	39.0	33.0	39.0
10	37.020849748819046	39.0	37.0	39.0	33.0	39.0
11	37.08563214193742	39.0	37.0	39.0	33.0	39.0
12	36.91380861049079	39.0	37.0	39.0	32.0	39.0
13	36.957971872345496	39.0	37.0	39.0	33.0	39.0
14	37.90425720007226	40.0	37.0	41.0	32.0	41.0
15	37.91071256980985	40.0	37.0	41.0	33.0	41.0
16	37.80852593102229	40.0	37.0	41.0	32.0	41.0
17	37.919595189317796	40.0	37.0	41.0	33.0	41.0
18	37.92066371732437	40.0	37.0	41.0	33.0	41.0
19	38.01354109412655	40.0	37.0	41.0	33.0	41.0
20	37.9242440548716	40.0	37.0	41.0	32.0	41.0
21	37.772003797502414	40.0	37.0	41.0	32.0	41.0
22	37.682207086877476	40.0	37.0	41.0	32.0	41.0
23	37.46977756936784	39.0	36.0	41.0	32.0	41.0
24	37.605374926490654	40.0	36.0	41.0	32.0	41.0
25	37.5384766173017	40.0	36.0	41.0	32.0	41.0
26	37.31078213943906	39.0	36.0	41.0	31.0	41.0
27	37.267918023146315	39.0	36.0	41.0	31.0	41.0
28	37.097558913176336	39.0	35.0	41.0	31.0	41.0
29	36.856361777446374	39.0	35.0	41.0	31.0	41.0
30	36.638241771757805	39.0	35.0	40.0	30.0	41.0
31	36.31742584684688	38.0	35.0	40.0	30.0	41.0
32	36.154187053899165	38.0	35.0	40.0	30.0	41.0
33	35.95344408100826	38.0	35.0	40.0	30.0	41.0
34	35.718733064023276	38.0	35.0	40.0	29.0	41.0
35	35.49342739967175	38.0	35.0	40.0	27.0	41.0
36	35.37714618462473	38.0	35.0	40.0	26.0	41.0
37	35.17003432357949	38.0	34.0	40.0	25.0	41.0
38	35.01427522667784	38.0	34.0	40.0	24.0	41.0
39	34.83224879021874	38.0	34.0	40.0	24.0	41.0
40	34.59383059603107	38.0	33.0	40.0	23.0	41.0
41	34.41512889599532	38.0	33.0	40.0	21.0	41.0
42	34.03002640571009	38.0	33.0	40.0	18.0	41.0
43	33.59287161136329	37.0	33.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	1.0
15	1.0
16	4.0
17	11.0
18	28.0
19	74.0
20	176.0
21	346.0
22	656.0
23	1187.0
24	1946.0
25	2961.0
26	4456.0
27	6449.0
28	8570.0
29	11507.0
30	14255.0
31	16956.0
32	20624.0
33	25749.0
34	31835.0
35	39177.0
36	51774.0
37	77944.0
38	93537.0
39	110117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.59629628206065	21.226231978198953	15.956813019129726	21.220658720610675
2	18.378681713180946	24.6355281718562	36.17024956663118	20.815540548331672
3	19.162012676278295	24.399529540187032	34.85784349523967	21.580614288295006
4	12.713561465343947	16.10363952938644	36.14084582832061	35.04195317694901
5	11.208589735212609	39.74328422460612	34.80249528194918	14.245630758232085
6	30.97040023676736	38.31998954533749	12.778326562145667	17.93128365574949
7	23.908121965937788	35.44245899812047	20.725791883030777	19.92362715291097
8	27.68102517190617	34.127554569879045	18.706543004408637	19.48487725380615
9	23.83317126044025	14.582716751674859	22.76541197904455	38.81870000884034
10	16.354820483451267	28.71419181999531	32.20401197673838	22.726975719815044
11	32.328353275345826	22.845935942130367	24.029004001214584	20.796706781309215
12	21.742238758355082	28.984206541082596	30.472074135856804	18.80148056470552
13	33.13013364287334	21.529301882223614	26.510064534479245	18.8304999404238
14	19.69300959753393	24.07224479284778	29.612447198188885	26.62229841142941
15	21.30137486499264	31.080327938163748	26.917681063608168	20.70061613323545
16	20.095437231666864	29.270172309750127	27.42907549265675	23.205314965926256
17	20.874540206248966	27.394482859350195	27.375649092327738	24.3553278420731
18	23.04849502826987	25.066014275226678	31.356492460727754	20.5289982357757
19	22.370671596757514	24.463333730508012	33.22411029668949	19.941884376044985
20	23.418251842057725	23.884291485215492	32.641224425474014	20.056232247252765
21	21.598487148836725	24.98933393806381	34.030695196620684	19.38148371647878
22	19.56213413485746	25.600662641109118	33.198358003005715	21.638845221027708
23	21.284462910931655	25.630258560715834	34.21884068554912	18.86643784280339
24	20.615287637745944	25.733075554154766	31.61843556737684	22.03320124072245
25	20.60221930960791	26.10360109312721	33.074977610878996	20.219201986385876
26	19.950340353075475	27.640282737122888	32.79247110554213	19.616905804259506
27	18.4055870946416	27.426577135806834	32.47729377986017	21.690541989691393
28	19.539648923208198	26.64593671085555	32.28472812112034	21.52968624481591
29	19.69762194864147	26.48546532857236	31.220235921759148	22.596676801027016
30	20.034515760788096	26.771238915943744	33.87137690211438	19.32286842115378
31	21.20547639821502	26.619992235875635	33.225263384466366	18.949267981442976
32	20.395624416249312	26.632676201421372	31.89017992012945	21.08151946219986
33	19.77430228580434	26.108982169419342	33.01040469537343	21.10631084940289
34	18.578165898582085	27.03241329740824	34.00513508423306	20.38428571977661
35	17.981250792747847	29.85517217522322	32.20381979544223	19.959757236586707
36	21.1213009905024	26.63997909067498	31.828105361473803	20.410614557348822
37	19.872506928135728	28.138993200625745	31.707607688789295	20.280892182449236
38	19.60037821279082	25.680994422898785	33.600209093250214	21.11841827106019
39	18.89199795519101	25.65255159106895	34.46118129999116	20.99426915374888
40	17.26614418978287	26.01327588393787	34.35586594970231	22.364713976576944
41	19.077645087269527	25.33160882650257	34.27053745421281	21.3202086320151
42	18.13691764262735	27.699474576336332	33.21834485780506	20.945262923231258
43	18.009693624577682	27.949310261328126	32.15404483974002	21.886951274354175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	157.0
1	163.0
2	169.0
3	401.5
4	634.0
5	634.0
6	999.0
7	1364.0
8	1495.0
9	1626.0
10	2436.5
11	3247.0
12	3247.0
13	5995.5
14	8744.0
15	15407.5
16	22071.0
17	19914.0
18	17757.0
19	17757.0
20	17416.0
21	17075.0
22	12948.0
23	8821.0
24	9419.0
25	10017.0
26	10017.0
27	10592.0
28	11167.0
29	11655.5
30	12144.0
31	13157.5
32	14171.0
33	14171.0
34	15654.5
35	17138.0
36	18656.5
37	20175.0
38	21483.0
39	22791.0
40	22791.0
41	23592.0
42	24393.0
43	25938.0
44	27483.0
45	31060.0
46	34637.0
47	34637.0
48	50146.0
49	65655.0
50	58751.5
51	51848.0
52	45140.0
53	38432.0
54	38432.0
55	33011.5
56	27591.0
57	22404.0
58	17217.0
59	15155.0
60	13093.0
61	13093.0
62	11286.0
63	9479.0
64	8211.0
65	6943.0
66	5927.0
67	4911.0
68	4911.0
69	4146.0
70	3381.0
71	2918.0
72	2455.0
73	2014.0
74	1573.0
75	1573.0
76	1230.5
77	888.0
78	705.0
79	522.0
80	397.0
81	272.0
82	272.0
83	200.0
84	128.0
85	112.0
86	96.0
87	70.5
88	45.0
89	45.0
90	31.5
91	18.0
92	13.5
93	9.0
94	6.0
95	3.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	520342.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.87105125659414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.65929067518175	47.85874619916576
2	7.875717908206139	8.800492778637231
3	2.406021068800782	4.032807793782417
4	1.1551465217003976	2.581570020911974
5	0.6520606088640108	1.8215655850123567
6	0.4332149266177503	1.45225040221092
7	0.3287154261980204	1.285597349815995
8	0.22265509910034878	0.9951979563502108
9	0.19403367597076385	0.9756778910101133
>10	0.9511003659550623	9.513826006397352
>50	0.06274564316936823	2.4772724246553928
>100	0.047231610420151775	5.790511120038682
>500	0.006895125608781281	2.625589018603129
>1k	0.004137075365268768	4.201982572139041
>5k	6.89512560878128E-4	3.2920506138174805
>10k+	3.44756280439064E-4	2.294862267451961
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11914	2.289647962301717	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8592	1.65122169649961	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8499	1.6333488359578892	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3264	0.6272797506255501	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2600	0.49967136998358774	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2198	0.4224144889322792	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2085	0.40069800246760784	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2021	0.38839839951416566	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1774	0.34092961936572486	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1773	0.3407374380695773	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1688	0.324402027897037	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1255	0.24118752666515483	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1090	0.20947761280081176	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1045	0.20082945447417277	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1022	0.1964092846627795	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	932	0.17911296800950144	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	884	0.16988826579441985	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	809	0.1554746685833548	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	790	0.15182322395655165	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	763	0.14663432896056824	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	748	0.14375160951835525	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	723	0.1389470771146669	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	712	0.136833082857044	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	707	0.13587217637630633	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	701	0.13471908859942114	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	658	0.1264552928650772	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	646	0.12414911731130679	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	634	0.12184294175753639	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	630	0.12107421657294626	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	587	0.11281042083860231	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	574	0.11031206398868436	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	570	0.10954333880409423	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	556	0.10685280065802875	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0011530877768852023	0.0
12	0.0	0.0	0.0	0.002113994257622871	0.0
13	0.0	0.0	0.0	0.002113994257622871	0.0
14	0.0	0.0	0.0	0.002882719442213006	0.0
15	0.0	0.0	0.0	0.004227988515245742	0.0
16	0.0	0.0	0.0	0.005957620180573546	0.0
17	0.0	0.0	0.0	0.009224702215081619	0.0
18	0.0	0.0	0.0	0.01076215258426189	0.0
19	0.0	0.0	0.0	0.013068328138032295	0.0
20	0.0	0.0	0.0	0.023446118129999118	0.0
21	0.0	0.0	0.0	0.04170334126401482	0.0
22	0.0	0.0	0.0	0.06783999754007941	0.0
23	0.0	0.0	0.0	0.08110050697425923	0.0
24	0.0	0.0	0.0	0.10147172436589781	0.0
25	0.0	0.0	0.0	0.1110807891732745	0.0
26	0.0	0.0	0.0	0.1235725734228642	0.0
27	0.0	0.0	0.0	0.19314220262827142	0.0
28	0.0	0.0	0.0	0.3755222526722809	0.0
29	0.0	0.0	0.0	0.6714814487394829	0.0
30	0.0	0.0	0.0	1.094280300264057	0.0
31	0.0	0.0	0.0	1.6222023207813323	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGGATT	40	0.0019297748	23.125	28
CCCTAGA	40	0.0019297748	23.125	2
GGTATCA	2805	0.0	22.490194	1
GGACCCT	275	0.0	22.2	6
TATACTG	50	2.6993488E-4	22.2	5
GTATTGG	170	0.0	21.764706	1
GACCCTC	290	0.0	21.051723	7
ATTGGAC	175	0.0	20.085714	3
GTACGGA	65	6.892852E-5	19.923077	3
TTGGACC	310	0.0	19.096773	4
TGGACCC	320	0.0	18.5	5
TAGCACC	50	0.0070298347	18.5	4
GCCCTAG	50	0.0070298347	18.5	1
TATTAAG	60	9.227559E-4	18.5	2
GACTTGC	65	0.0015783628	17.076923	8
ACCCTCG	360	0.0	16.958334	8
TATTGGA	210	0.0	16.738094	2
TACTGTG	90	4.4384353E-5	16.444445	7
GTTTAAG	80	3.3781168E-4	16.1875	1
CGTGTCT	80	3.3781168E-4	16.1875	35
>>END_MODULE