FastQCFastQC Report
Wed 25 May 2016
SRR2088533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331908
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT197421.4822345086897895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT144321.0835583238481936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT143501.0774017424626925No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57050.42833288785711926No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG34190.2566994116710764No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG27700.20797232241265914No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA26650.20008889502878577No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA25770.1934818320784919No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA25750.19333167155689432No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG21630.16239860410779122No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21370.1604465173270226No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17890.1343185865690423No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC16900.1268856407499617No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT14300.1073647729422753No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14110.10593824798709821No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGG3750.023.1866651
GGTATCA43200.022.9965291
ATTGGAC3950.021.0759493
TCTATAC1251.8189894E-1120.723
ACGTTCG1550.020.29032122
CGCACTA656.903184E-519.92307729
CGTTCGC1800.019.52777923
TAAACGT951.6766171E-719.4736844
TTGGACC5650.019.3185844
CGTCTTA500.007035453318.533
GACCCTC5750.018.339137
GGACCCT5950.018.0336136
ACCCTCG5700.017.8508768
TAGGTCG650.001580112417.07692321
TGGACCC6700.016.8432855
TATTGGA5550.016.6666662
TTCGTTA1851.8189894E-1116.00000230
CGCGCAA700.002592783715.8571429
ATTATAC2250.015.6222223
CAGTTCG2150.015.48837213
Produced by FastQC (version 0.11.5)